AUTHOR=Peng Lisha , Zhou Lili , Li Qinfei , Wei Dayong , Ren Xuesong , Song Hongyuan , Mei Jiaqin , Si Jun , Qian Wei 

TITLE=Identification of Quantitative Trait Loci for Clubroot Resistance in Brassica oleracea With the Use of Brassica SNP Microarray

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00822

DOI=10.3389/fpls.2018.00822

ISSN=1664-462X

ABSTRACT=<p>Increasing clubroot resistance (CR) of <italic>Brassica oleracea</italic> by ascertaining the molecular mechanisms has been the key focus in modern <italic>B. oleracea</italic> breeding. In order to identify the quantitative trait loci (QTLs) associated with CR in <italic>B. oleracea</italic>, 94 F2 vegetative lines which were developed by tissue culture of selfed seeds from the F1 generation between a clubroot-resistant <italic>B. oleracea</italic> inbred line and a susceptible line, were identified for disease incidence and six CR-associated traits under a lab inoculation by <italic>Plasmodiophora brassicae</italic> and were genotyped with the 60K <italic>Brassica</italic> SNP array. Significant correlations were detected for numbers of fibrous roots and <italic>P. brassicae</italic> content in roots with disease incidence. Nine linkage groups were constructed from 565 bins which covered around 3,000 SNPs, spanning 1,028 cM of the <italic>B. oleracea</italic> genome with an average distance of 1.82 cM between adjacent bins. A total of 23 QTLs were identified for disease incidence and the other two correlated traits, individually explaining 6.1–17.8% of the phenotypic variation. Several overlaps were detected among traits, including one three-traits-overlapped locus on linkage group C08 and two important overlapped regions between the two CR-associated traits on C06. The QTLs were compared with known CR loci/genes and the novelty of our QTLs was discussed.</p>