AUTHOR=Wang Hongjin , Zhang Hongjun , Li Bin , Yu Zhihui , Li Guangrong , Zhang Jie , Yang Zujun 

TITLE=Molecular Cytogenetic Characterization of New Wheat—Dasypyrum breviaristatum Introgression Lines for Improving Grain Quality of Wheat

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00365

DOI=10.3389/fpls.2018.00365

ISSN=1664-462X

ABSTRACT=<p>As an important relative of wheat (<italic>Triticum aestivum</italic> L), <italic>Dasypyrum breviaristatum</italic> contains novel high molecular weight glutenin subunits (HMW-GSs) encoded by <italic>Glu-1Vb</italic> genes. We identified new wheat—<italic>D. breviaristatum</italic> chromosome introgression lines including chromosomes 1V<sup>b</sup> and 1V<sup>b</sup>L.5V<sup>b</sup>L by fluorescence <italic>in situ</italic> hybridization (FISH) combined with molecular markers. We found that chromosome changes occurred in the wheat—<italic>D. breviaristatum</italic> introgression lines and particularly induced the deletion of 5BS terminal repeats and formation of a new type of 5B-7B reciprocal translocation. The results imply that the <italic>D. breviaristatum</italic> chromosome 1V<sup>b</sup> may contain genes which induce chromosomal recombination in wheat background. Ten putative high molecular weight glutenin subunit (HMW-GS) genes from <italic>D. breviaristatum</italic> and wheat—<italic>D. breviaristatum</italic> introgression lines were isolated. The lengths of the HMW-GS genes in <italic>Dasypyrum</italic> were significantly shorter than typical HMW-GS of common wheat. A new y-type HMW-GS gene, named <italic>Glu-Vb1y</italic>, was characterized in wheat—<italic>D. breviaristatum</italic> 1V<sup>b</sup> introgression lines. The new wheat—<italic>D. breviaristatum</italic> germplasm displayed reduced plant height, increased tillers and superior grain protein and gluten contents, improved gluten performance index. The results showed considerable potential for utilization of <italic>D. breviaristatum</italic> chromosome 1V<sup>b</sup> segments in future wheat improvement.</p>