AUTHOR=Liu Dongming , Dong Xin , Liu Zezhou , Tang Jun , Zhuang Mu , Zhang Yangyong , Lv Honghao , Liu Yumei , Li Zhansheng , Fang Zhiyuan , Yang Limei 

TITLE=Fine Mapping and Candidate Gene Identification for Wax Biosynthesis Locus, BoWax1 in Brassica oleracea L. var. capitata

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00309

DOI=10.3389/fpls.2018.00309

ISSN=1664-462X

ABSTRACT=<p>Cuticular waxes play important roles in plant protection against various biotic and abiotic environmental stresses. The cuticular wax covering gives normal cabbage a glaucous appearance, but the appearance of waxless mutant is glossy green. Based on the present study, inheritance of the glossy green character of mutant HUAYOU2 follows a simple recessive pattern. Genetic analysis of an F<sub>2</sub> population comprising 808 recessive individuals derived from HUAYOU2 (P<sub>1</sub>, maternal parent) and M36 (P<sub>2</sub>, paternal parent) revealed that a single recessive locus, <italic>BoWax1</italic> (<italic>Brassica oleracea Wax 1</italic>), controls glossy green trait in <italic>B</italic>. <italic>oleracea</italic>. This locus was mapped to a region of 158.5 kb on chromosome C01. Based on nucleotide sequence analysis, <italic>Bol013612</italic> was identified as the candidate gene for <italic>BoWax1</italic>. Sequencing results demonstrated that there is a deletion mutation of two nucleotides in the cDNA of <italic>Bol013612</italic> of HUAYOU2, which may account for its glossy green trait. These results lay the foundation for functional analysis of <italic>BoWax1</italic> and may accelerate research on wax metabolism in cabbage.</p>