AUTHOR=Barilli Eleonora , Cobos María J. , Carrillo Estefanía , Kilian Andrzej , Carling Jason , Rubiales Diego 

TITLE=A High-Density Integrated DArTseq SNP-Based Genetic Map of Pisum fulvum and Identification of QTLs Controlling Rust Resistance

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00167

DOI=10.3389/fpls.2018.00167

ISSN=1664-462X

ABSTRACT=<p><italic>Pisum fulvum</italic>, a wild relative of pea is an important source of allelic diversity to improve the genetic resistance of cultivated species against fungal diseases of economic importance like the pea rust caused by <italic>Uromyces pisi</italic>. To unravel the genetic control underlying resistance to this fungal disease, a recombinant inbred line (RIL) population was generated from a cross between two <italic>P. fulvum</italic> accessions, <ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="IFPI3260" xmlns:xlink="http://www.w3.org/1999/xlink">IFPI3260</ext-link> and <ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="IFPI3251" xmlns:xlink="http://www.w3.org/1999/xlink">IFPI3251</ext-link>, and genotyped using Diversity Arrays Technology. A total of 9,569 high-quality DArT-Seq and 8,514 SNPs markers were generated. Finally, a total of 12,058 markers were assembled into seven linkage groups, equivalent to the number of haploid chromosomes of <italic>P. fulvum</italic> and <italic>P. sativum</italic>. The newly constructed integrated genetic linkage map of <italic>P. fulvum</italic> covered an accumulated distance of 1,877.45 cM, an average density of 1.19 markers cM<sup>−1</sup> and an average distance between adjacent markers of 1.85 cM. The composite interval mapping revealed three QTLs distributed over two linkage groups that were associated with the percentage of rust disease severity (DS%). QTLs <italic>UpDSII</italic> and <italic>UpDSIV</italic> were located in the LGs II and IV respectively and were consistently identified both in adult plants over 3 years at the field (Córdoba, Spain) and in seedling plants under controlled conditions. Whenever they were detected, their contribution to the total phenotypic variance varied between 19.8 and 29.2. A third QTL (<italic>UpDSIV.2</italic>) was also located in the LGIVand was environmentally specific as was only detected for DS % in seedlings under controlled conditions. It accounted more than 14% of the phenotypic variation studied. Taking together the data obtained in the study, it could be concluded that the expression of resistance to fungal diseases in <italic>P. fulvum</italic> originates from the resistant parent IFPI3260.</p>