AUTHOR=Baudisch Bianca , Pfort Ingrid , Sorge Eberhard , Conrad Udo TITLE=Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants JOURNAL=Frontiers in Plant Science VOLUME=9 YEAR=2018 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00130 DOI=10.3389/fpls.2018.00130 ISSN=1664-462X ABSTRACT=

Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation via the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest in planta using specific, high-affinity binding proteins.