AUTHOR=Li Bingjuan , Xiao Guohui , Luo Kaisheng , Wang Zhengyi , Mao Bizeng , Lin Xinchun , Guo Xiaoqin 

TITLE=Overexpression of PvGF14c from Phyllostachys violascens Delays Flowering Time in Transgenic Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2018.00105

DOI=10.3389/fpls.2018.00105

ISSN=1664-462X

ABSTRACT=<p>14-3-3 Proteins are a family of highly conserved regulatory molecules expressed in all eukaryotic cells and regulate a diverse set of biological responses in plants. However, their functions in flowering of <italic>Phyllostachys violascens</italic> are poorly understood. In this study, four non-𝜀 <italic>Pv14-3-3</italic> genes from <italic>P. violascens</italic> were identified and named <italic>PvGF14b</italic>, <italic>PvGF14c</italic>, <italic>PvGF14e</italic>, and <italic>PvGF14f</italic>. qRT-PCR analyses revealed that <italic>PvGF14b</italic> and <italic>PvGF14e</italic> exhibited widely expressed in all tested bamboo tissues. <italic>PvGF14b</italic> was highest expression in root and lowest in immature leaf. Whereas <italic>PvGF14c</italic> and <italic>PvGF14f</italic> showed tissue-specific expression. <italic>PvGF14c</italic> was mainly expressed in immature and mature leaves. <italic>PvGF14f</italic> was highest expression in mature leaves. These four genes were not significantly differentially expressed in mature leaf before bamboo flowering and during flower development. <italic>PvGF14b</italic> and <italic>PvGF14c</italic> were not induced by circadian rhythm. PvGF14c displayed subcellular localization in the cytoplasm and PvFT in nucleus and cytoplasm. Yeast two-hybrid screening and bimolecular fluorescence complementation confirmed the interaction between PvGF14c and PvFT. The overexpression of <italic>PvGF14b</italic>, <italic>PvGF14c</italic>, and <italic>PvGF14e</italic> significantly delayed flowering time in transgenic <italic>Arabidopsis</italic> under long-day condition. These findings suggested that at least three <italic>PvGF14</italic> genes are involved in flowering and may act as a negative regulator of flowering by interacting with PvFT in bamboo.</p>