AUTHOR=He Fang , Xing Piyi , Bao Yinguang , Ren Mingjian , Liu Shubing , Wang Yuhai , Li Xingfeng , Wang Honggang 

TITLE=Chromosome Pairing in Hybrid Progeny between Triticum aestivum and Elytrigia elongata

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.02161

DOI=10.3389/fpls.2017.02161

ISSN=1664-462X

ABSTRACT=<p>In this study, the intergeneric hybrids F<sub>1</sub>, F<sub>2</sub>, BC<sub>1</sub>F<sub>1</sub>, BC<sub>1</sub>F<sub>2</sub>, and BC<sub>2</sub>F<sub>1</sub> from <italic>Elytrigia elongata</italic> and <italic>Triticum aestivum</italic> crosses were produced to study their chromosome pairing behavior. The average <italic>E. elongata</italic> chromosome configuration of the two F<sub>1</sub> hybrids agreed with the theoretical chromosome configuration of 21I+7II, indicating that the genomic constitution of this F<sub>1</sub> hybrid was ABDStStE<sup>e</sup>E<sup>b</sup>E<sup>x</sup>. Compared with the BC<sub>1</sub>F<sub>1</sub> generation, the BC<sub>2</sub>F<sub>1</sub> generation showed a rapid decrease in the number of <italic>E. elongata</italic> chromosomes and the BC<sub>1</sub>F<sub>2</sub> generation showed a more extensive distribution of <italic>E. elongata</italic> chromosomes. In addition, pairing between wheat and <italic>E. elongata</italic> chromosomes was detected in each of the wheat<italic>-E. elongata</italic> hybrid progenies, albeit rarely. Our results demonstrated that genomic <italic>in situ</italic> hybridization (GISH) using an <italic>E. elongata</italic> genomic DNA probe offers a reliable approach for characterizing chromosome pairing in wheat and <italic>E. elongata</italic> hybrid progenies.</p>