AUTHOR=Moyle Richard L. , Carvalhais Lilia C. , Pretorius Lara-Simone , Nowak Ekaterina , Subramaniam Gayathery , Dalton-Morgan Jessica , Schenk Peer M. TITLE=An Optimized Transient Dual Luciferase Assay for Quantifying MicroRNA Directed Repression of Targeted Sequences JOURNAL=Frontiers in Plant Science VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.01631 DOI=10.3389/fpls.2017.01631 ISSN=1664-462X ABSTRACT=
Studies investigating the action of small RNAs on computationally predicted target genes require some form of experimental validation. Classical molecular methods of validating microRNA action on target genes are laborious, while approaches that tag predicted target sequences to qualitative reporter genes encounter technical limitations. The aim of this study was to address the challenge of experimentally validating large numbers of computationally predicted microRNA-target transcript interactions using an optimized, quantitative, cost-effective, and scalable approach. The presented method combines transient expression via agroinfiltration of