AUTHOR=Choi Nae Young , Lee Eunhye , Lee Sang Gu , Choi Chang Hyun , Park Sang Ryeol , Ahn Ilpyung , Bae Shin Chul , Hwang Cheol Ho , Hwang Duk-Ju 

TITLE=Genome-Wide Expression Profiling of OsWRKY Superfamily Genes during Infection with Xanthomonas oryzae pv. oryzae Using Real-Time PCR

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.01628

DOI=10.3389/fpls.2017.01628

ISSN=1664-462X

ABSTRACT=<p>WRKY transcription factors (TFs) are involved in regulating a range of biological processes such as growth, development, and the responses to biotic and abiotic stresses. Genome-wide expression profiling of <italic>OsWRKY</italic> TF superfamily genes in rice after infection with <italic>Xanthomonas oryzae</italic> pv. <italic>oryzae</italic> (<italic>Xoo</italic>) was performed to elucidate the function of OsWRKY TFs in the interaction between rice and <italic>Xoo</italic>. Of the 111 <italic>OsWRKY</italic> TF genes tested, the transcription of 94 genes changed after <italic>Xoo</italic> infection. The <italic>OsWRKY</italic> TF genes were classified into eight types according to their expression profiles. Eighty-two genes in Groups I, II, III, IV, VII were up-regulated after exposure to a compatible or an incompatible race of <italic>Xoo</italic>. Examination of salicylic acid (SA)-deficient rice lines revealed that SA was involved in Xa1-mediated resistance to <italic>Xoo</italic> infection. <italic>OsWRKY</italic> TF genes involved in Xa1-mediated resistance were classified according to their SA-dependent or -independent expression. In SA-deficient rice, the expression of 12 of 57 OsWRKY TF genes involved in Xa1-mediated resistance was compromised. Of these six OsWRKY TF genes were induced by SA. <italic>OsWRKY88</italic>, an example of a gene possibly involved in SA-dependent Xa1-mediated resistance, activated defense related genes and increased resistance to <italic>Xoo</italic>. Thus, expression profiling of <italic>OsWRKY</italic> TF genes may help predict the functions of <italic>OsWRKY</italic> TF genes involved in Xa1-mediated resistance.</p>