AUTHOR=Cheng Pengfei , Wang Yuanzhu , Liu Tianzhong , Liu Defu 

TITLE=Biofilm Attached Cultivation of Chlorella pyrenoidosa Is a Developed System for Swine Wastewater Treatment and Lipid Production

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.01594

DOI=10.3389/fpls.2017.01594

ISSN=1664-462X

ABSTRACT=<p>This study showed the new potential of using soluble contents and heavy metals in swine wastewater as nutrient supplements for the algae <italic>Chlorella pyrenoidosa</italic> with biofilm attached method. Algae with biofilm attached cultivation grew well in unpasteurized wastewater reaching a biomass productivity of 5.03 g m<sup>−2</sup> d<sup>−1</sup>, lipid content of 35.9% and lipid productivity of 1.80 g m<sup>−2</sup> d<sup>−1</sup>. <italic>Chlorella</italic> grew in BG11 medium delivered lower values for each of the aforementioned parameters. The FAMEs compositions in the algae paste were mainly consisted of C16:0, C18:2, and C18:3. Algae removed NH<sub>4</sub><sup>+</sup>–N, total phosphorus (TP), and COD by 75.9, 68.4, and 74.8%, respectively. Notably, Zn<sup>2+</sup>, Cu<sup>+</sup>, and Fe<sup>2+</sup> were removed from wastewater with a ratio of 65.71, 53.64, and 58.89%, respectively. Biofilm attached cultivation of <italic>C. pyrenoidosa</italic> in swine wastewater containing heavy metals could accumulate considerable biomass and lipid, and the removal ratio of NH<sub>4</sub><sup>+</sup>–N, TP, COD, and as well as heavy metal were high. Treatment of wastewater with biofilm attached cultivation showed an increasingly popular for the concentration of microalgae and environmental sustainability.</p>