AUTHOR=Fina Julieta P. , Masotti Fiorella , Rius Sebastián P. , Crevacuore Franco , Casati Paula 

TITLE=HAC1 and HAF1 Histone Acetyltransferases Have Different Roles in UV-B Responses in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.01179

DOI=10.3389/fpls.2017.01179

ISSN=1664-462X

ABSTRACT=<p>Arabidopsis has 12 histone acetyltransferases grouped in four families: the GNAT/HAG, the MYST/HAM, the p300/CBP/HAC and the TAFII250/HAF families. We previously showed that <italic>ham1</italic> and <italic>ham2</italic> mutants accumulated higher damaged DNA after UV-B exposure than WT plants. In contrast, <italic>hag3</italic> RNA interference transgenic plants showed less DNA damage and lower inhibition of plant growth by UV-B, and increased levels of UV-B-absorbing compounds. These results demonstrated that HAM1, HAM2, and HAG3 participate in UV-B-induced DNA damage repair and signaling. In this work, to further explore the role of histone acetylation in UV-B responses, a putative function of other acetyltransferases of the HAC and the HAF families was analyzed. Neither HAC nor HAF acetyltrasferases participate in DNA damage and repair after UV-B radiation in Arabidopsis. Despite this, <italic>haf1</italic> mutants presented lower inhibition of leaf and root growth by UV-B, with altered expression of <italic>E2F</italic> transcription factors. On the other hand, <italic>hac1</italic> plants showed a delay in flowering time after UV-B exposure and changes in <italic>FLC</italic> and <italic>SOC1</italic> expression patterns. Our data indicate that HAC1 and HAF1 have crucial roles for in UV-B signaling, confirming that, directly or indirectly, both enzymes also have a role in UV-B responses.</p>