AUTHOR=Bao Fei , Du Dongliang , An Yang , Yang Weiru , Wang Jia , Cheng Tangren , Zhang Qixiang 

TITLE=Overexpression of Prunus mume Dehydrin Genes in Tobacco Enhances Tolerance to Cold and Drought

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00151

DOI=10.3389/fpls.2017.00151

ISSN=1664-462X

ABSTRACT=<p>Dehydrins, known as group 2 or D-11 family late-embryogenesis-abundant (LEA) proteins, play important roles in plant growth and stress tolerance. Six dehydrin genes were previously identified from the genome of <italic>Prunus mume</italic>. In this study, five of them (<italic>PmLEA8, PmLEA10, PmLEA19, PmLEA20</italic>, and <italic>PmLEA29</italic>) were cloned from cold-resistant <italic>P. mume</italic> ‘Beijingyudie’. Real-time RT-PCR analysis indicated that all these genes could be up-regulated by one or several treatments (ABA, SA, low temperature, high temperature, PEG, and NaCl treatments). The results of spot assay demonstrated that the expression of all these dehydrins, except PmLEA8, conferred improved osmotic and freezing-resistance to the recombinant <italic>Escherichia coli</italic>. So four dehydrin genes, <italic>PmLEA10, PmLEA19, PmLEA20</italic> and <italic>PmLEA29</italic> were chosen for individual over-expression in tobacco plants. The transgenic tobacco plants showed lower relative content of malondialdehyde, relative electrolyte leakage and higher relative content of water than control plants when exposed to cold and drought stress. These results demonstrated that <italic>PmLEAs</italic> were involved in plant responses to cold and drought.</p>