AUTHOR=Ding Wona , Tong Huishan , Zheng Wenjuan , Ye Jing , Pan Zhichong , Zhang Botao , Zhu Shihua 

TITLE=Isolation, Characterization and Transcriptome Analysis of a Cytokinin Receptor Mutant Osckt1 in Rice

JOURNAL=Frontiers in Plant Science

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00088

DOI=10.3389/fpls.2017.00088

ISSN=1664-462X

ABSTRACT=<p>Cytokinins play important roles in regulating plant development, including shoot and root meristems, leaf longevity, and grain yield. However, the <italic>in planta</italic> functions of rice cytokinin receptors have not been genetically characterized yet. Here we isolated a rice mutant, <italic>Osckt1</italic>, with enhanced tolerance to cytokinin treatment. Further analysis showed that <italic>Osckt1</italic> was insensitive to aromatic cytokinins but responded normally to isoprenoid and phenylurea-type cytokinins. Map-based cloning revealed that the mutation occurred in a putative cytokinin receptor gene, histidine kinase 6 (<italic>OsHK6</italic>). <italic>OsCKT1</italic> was found to be expressed in various tissues throughout the plant and the protein was located in the endoplasmic reticulum. In addition, whole-genome gene expression profiling analysis showed that <italic>OsCKT1</italic> was involved in cytokinin regulation of a number of biological processes, including secondary metabolism, sucrose and starch metabolism, chlorophyll synthesis, and photosynthesis. Our results demonstrate that <italic>OsCKT1</italic> plays important roles in cytokinin perception and control of root development in rice.</p>