AUTHOR=Agarwal Parinita , Dabi Mitali , Sapara Komal K. , Joshi Priyanka S. , Agarwal Pradeep K. 

TITLE=Ectopic Expression of JcWRKY Transcription Factor Confers Salinity Tolerance via Salicylic Acid Signaling

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01541

DOI=10.3389/fpls.2016.01541

ISSN=1664-462X

ABSTRACT=<p>Plants, being sessile, have developed intricate signaling network to specifically respond to the diverse environmental stress. The plant-specific WRKY TFs form one of the largest TF family and are involved in diverse plant processes, involving growth, development and stress signaling through auto and cross regulation with different genes and TFs. Here, we report the functional characterization of a salicylic acid -inducible JcWRKY TF. The JcWRKY overexpression confers salinity tolerance in transgenic tobacco, as was evident by increased chlorophyll content and seed germination potential. The transgenic plants showed increased soluble sugar, membrane stability, reduced electrolyte leakage and generation of reactive oxygen species (H<sub>2</sub>O<sub>2</sub> and <inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mi mathvariant="normal" mathcolor="black">O</mml:mi><mml:mi mathvariant="normal" mathcolor="black">2</mml:mi><mml:mn mathvariant="normal" mathcolor="black">•-</mml:mn></mml:msubsup></mml:math></inline-formula>) as compared to the wild type. Furthermore, the low SA treatment along with salinity improved the tolerance potential of the transgenics by maintaining ROS homeostasis and high K<sup>+</sup>/Na<sup>+</sup> ratio. The transcript expression of SA biosynthetic gene <italic>ICS1</italic> and antioxidative enzymes (<italic>CAT</italic> and <italic>SOD</italic>) showed upregulation during stress. Thus, the present study reflects that JcWRKY is working in co-ordination with SA signaling to orchestrate the different biochemical and molecular pathways to maneuvre salt stress tolerance of the transgenic plants.</p>