AUTHOR=Wang Jingxue , Singh Sanjay K. , Du Chunfang , Li Chen , Fan Jianchun , Pattanaik Sitakanta , Yuan Ling 

TITLE=Comparative Transcriptomic Analysis of Two Brassica napus Near-Isogenic Lines Reveals a Network of Genes That Influences Seed Oil Accumulation

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01498

DOI=10.3389/fpls.2016.01498

ISSN=1664-462X

ABSTRACT=<p>Rapeseed (<italic>Brassica napus</italic>) is an important oil seed crop, providing more than 13% of the world’s supply of edible oils. An in-depth knowledge of the gene network involved in biosynthesis and accumulation of seed oil is critical for the improvement of <italic>B. napus</italic>. Using available genomic and transcriptomic resources, we identified 1,750 acyl-lipid metabolism (ALM) genes that are distributed over 19 chromosomes in the <italic>B</italic>. <italic>napus</italic> genome. <italic>B. rapa</italic> and <italic>B. oleracea</italic>, two diploid progenitors of <italic>B. napus</italic>, contributed almost equally to the ALM genes. Genome collinearity analysis demonstrated that the majority of the ALM genes have arisen due to genome duplication or segmental duplication events. In addition, we profiled the expression patterns of the ALM genes in four different developmental stages. Furthermore, we developed two <italic>B. napus</italic> near isogenic lines (NILs). The high oil NIL, YC13-559, accumulates significantly higher (∼10%) seed oil compared to the other, YC13-554. Comparative gene expression analysis revealed upregulation of lipid biosynthesis-related regulatory genes in YC13-559, including <italic>SHOOTMERISTEMLESS, LEAFY COTYLEDON 1 (LEC1), LEC2, FUSCA3, ABSCISIC ACID INSENSITIVE 3 (ABI3), ABI4, ABI5</italic>, and <italic>WRINKLED1</italic>, as well as structural genes, such as <italic>ACETYL-CoA CARBOXYLASE, ACYL-CoA DIACYLGLYCEROL ACYLTRANSFERASE</italic>, and <italic>LONG</italic>-<italic>CHAIN ACYL-CoA SYNTHETASES</italic>. We observed that several genes related to the phytohormones, gibberellins, jasmonate, and indole acetic acid, were differentially expressed in the NILs. Our findings provide a broad account of the numbers, distribution, and expression profiles of acyl-lipid metabolism genes, as well as gene networks that potentially control oil accumulation in <italic>B</italic>. <italic>napus</italic> seeds. The upregulation of key regulatory and structural genes related to lipid biosynthesis likely plays a major role for the increased seed oil in YC13-559.</p>