AUTHOR=Clevenger Josh , Chu Ye , Scheffler Brian , Ozias-Akins Peggy
TITLE=A Developmental Transcriptome Map for Allotetraploid Arachis hypogaea
JOURNAL=Frontiers in Plant Science
VOLUME=7
YEAR=2016
URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.01446
DOI=10.3389/fpls.2016.01446
ISSN=1664-462X
ABSTRACT=
The advent of the genome sequences of Arachis duranensis and Arachis ipaensis has ushered in a new era for peanut genomics. With the goal of producing a gene atlas for cultivated peanut (Arachis hypogaea), 22 different tissue types and ontogenies that represent the full development of peanut were sequenced, including a complete reproductive series from flower to peg elongation and peg tip immersion in the soil to fully mature seed. Using a genome-guided assembly pipeline, a homeolog-specific transcriptome assembly for Arachis hypogaea was assembled and its accuracy was validated. The assembly was used to annotate 21 developmental co-expression networks as tools for gene discovery. Using a set of 8816 putative homeologous gene pairs, homeolog expression bias was documented, and although bias was mostly balanced, there were striking differences in expression bias in a tissue-specific context. Over 9000 alterative splicing events and over 6000 non-coding RNAs were further identified and profiled in a developmental context. Together, this work represents a major new resource for cultivated peanut and will be integrated into peanutbase.org as an available resource for all peanut researchers.