AUTHOR=Jin Yazhong , Zhang Chong , Liu Wei , Tang Yufan , Qi Hongyan , Chen Hao , Cao Songxiao 

TITLE=The Alcohol Dehydrogenase Gene Family in Melon (Cucumis melo L.): Bioinformatic Analysis and Expression Patterns

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00670

DOI=10.3389/fpls.2016.00670

ISSN=1664-462X

ABSTRACT=<p>Alcohol dehydrogenases (ADH), encoded by multigene family in plants, play a critical role in plant growth, development, adaptation, fruit ripening and aroma production. Thirteen ADH genes were identified in melon genome, including 12 ADHs and one formaldehyde dehydrogenease (FDH), designated <italic>CmADH1-12</italic> and <italic>CmFDH1</italic>, in which <italic>CmADH1</italic> and <italic>CmADH2</italic> have been isolated in Cantaloupe. ADH genes shared a lower identity with each other at the protein level and had different intron-exon structure at nucleotide level. No typical signal peptides were found in all <italic>CmADHs</italic>, and <italic>CmADH</italic> proteins might locate in the cytoplasm. The phylogenetic tree revealed that 13 ADH genes were divided into three groups respectively, namely long-, medium-, and short-chain ADH subfamily, and <italic>CmADH1,3-11</italic>, which belongs to the medium-chain ADH subfamily, fell into six medium-chain ADH subgroups. <italic>CmADH12</italic> may belong to the long-chain ADH subfamily, while <italic>CmFDH1</italic> may be a Class III ADH and serve as an ancestral ADH in melon. Expression profiling revealed that <italic>CmADH1, CmADH2, CmADH10</italic> and <italic>CmFDH1</italic> were moderately or strongly expressed in different vegetative tissues and fruit at medium and late developmental stages, while <italic>CmADH8</italic> and <italic>CmADH12</italic> were highly expressed in fruit after 20 days. <italic>CmADH3</italic> showed preferential expression in young tissues. <italic>CmADH4</italic> only had slight expression in root. Promoter analysis revealed several motifs of <italic>CmADH</italic> genes involved in the gene expression modulated by various hormones, and the response pattern of <italic>CmADH</italic> genes to ABA, IAA and ethylene were different. These <italic>CmADHs</italic> were divided into ethylene-sensitive and –insensitive groups, and the functions of <italic>CmADHs</italic> were discussed.</p>