AUTHOR=Yu Chun-Wei , Chang Kao-Yuan , Wu Keqiang 

TITLE=Genome-Wide Analysis of Gene Regulatory Networks of the FVE-HDA6-FLD Complex in Arabidopsis

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00555

DOI=10.3389/fpls.2016.00555

ISSN=1664-462X

ABSTRACT=<p>FVE/MSI4 is a homolog of the mammalian RbAp48 protein. We found that FVE regulates flowering time by repressing <italic>FLC</italic> through decreasing histone H3K4 trimethylation and H3 acetylation. Furthermore, FVE interacts with the histone deacetylase HDA6 and the histone demethylase FLD, suggesting that these proteins may form a protein complex to regulate flowering time. To further investigate the function of the FVE-FLD-HDA6 complex, we compared the gene expression profiles of <italic>fve</italic>, <italic>fld</italic>, and <italic>hda6</italic> mutant plants by using RNA-seq analysis. Among the mis-regulated genes found in <italic>fve</italic> plants, 51.8 and 36.5% of them were also mis-regulated in <italic>fld</italic> and <italic>hda6</italic> plants, respectively, suggesting that FVE, HDA6, and FLD may regulate the gene expression in the same developmental processes in <italic>Arabidopsis</italic>. Gene ontology analysis revealed that among 383 genes co-regulated by FVE, HDA6, and FLD, 15.6% of them are involved in transcription, 8.2% in RNA metabolic process, 7.7% in response to abiotic stress, and 6.3% in hormone stimulus. Taken together, these results indicate that HDA6, FVE, and FLD co-regulate the gene expression in multiple development processes and pathways.</p>