AUTHOR=Song Aiping , Gao Tianwei , Li Peiling , Chen Sumei , Guan Zhiyong , Wu Dan , Xin Jingjing , Fan Qingqing , Zhao Kunkun , Chen Fadi 

TITLE=Transcriptome-Wide Identification and Expression Profiling of the DOF Transcription Factor Gene Family in Chrysanthemum morifolium

JOURNAL=Frontiers in Plant Science

VOLUME=7

YEAR=2016

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2016.00199

DOI=10.3389/fpls.2016.00199

ISSN=1664-462X

ABSTRACT=<p>The family of DNA binding with one finger (DOF) transcription factors is plant specific, and these proteins contain a highly conserved domain (DOF domain) of 50-52 amino acids that includes a C2C2-type zinc finger motif at the N-terminus that is known to function in a number of plant processes. Here, we characterized 20 <italic>DOF</italic> genes in the important ornamental species chrysanthemum (<italic>Chrysanthemum morifolium</italic>) based on transcriptomic sequences. Phylogenetic analysis identified one pair of putative orthologous proteins in <italic>Arabidopsis</italic> and chrysanthemum and six pairs of paralogous proteins in chrysanthemum. Conserved motifs in the DOF proteins shared by <italic>Arabidopsis</italic> and chrysanthemum were analyzed using MEME. Bioinformatics analysis revealed that 13 <italic>CmDOFs</italic> could be targeted by 16 miRNA families. Moreover, we used 5' RLM-RACE to map the cleavage sites in <italic>CmDOF3, 15</italic>, and <italic>21</italic>. The expression of these 20 genes in response to phytohormone treatments and abiotic stresses was characterized, and the expression patterns of six pairs of paralogous <italic>CmDOF</italic> genes were found to completely differ from one another, except for <italic>CmDOF6</italic> and <italic>CmDOF7</italic>. This work will promote our research of the various functions of <italic>DOF</italic> gene family members in plant hormone and stress responses.</p>