Gea Guerriero
Jean-Francois Hausman
Inés Ezcurra2*- 1Environmental Research and Innovation, Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg
- 2School of Biotechnology, Division of Industrial Biotechnology, KTH Royal Institute of Technology, AlbaNova University Center, Stockholm, Sweden
The metabolic complexity of living organisms relies on supramolecular protein structures which ensure vital processes, such as signal transduction, transcription, translation and cell wall synthesis. In eukaryotes WD40-repeat (WDR) proteins often function as molecular “hubs” mediating supramolecular interactions. WDR proteins may display a variety of interacting partners and participate in the assembly of complexes involved in distinct cellular functions. In plants, the formation of lignocellulosic biomass involves extensive synthesis of cell wall polysaccharides, a process that requires the assembly of large transmembrane enzyme complexes, intensive vesicle trafficking, interactions with the cytoskeleton, and coordinated gene expression. Because of their function as supramolecular hubs, WDR proteins could participate in each or any of these steps, although to date only few WDR proteins have been linked to the cell wall by experimental evidence. Nevertheless, several potential cell wall-related WDR proteins were recently identified using in silico approaches, such as analyses of co-expression, interactome and conserved gene neighborhood. Notably, some WDR genes are frequently genomic neighbors of genes coding for GT2-family polysaccharide synthases in eukaryotes, and this WDR-GT2 collinear microsynteny is detected in diverse taxa. In angiosperms, two WDR genes are collinear to cellulose synthase genes, CesAs, whereas in ascomycetous fungi several WDR genes are adjacent to chitin synthase genes, chs. In this Perspective we summarize and discuss experimental and in silico studies on the possible involvement of WDR proteins in plant cell wall formation. The prospects of biotechnological engineering for enhanced biomass production are discussed.
Plant Cell Wall Biosynthesis: A Multiplayer Matter
Plant lignocellulosic biomass is one of the most abundant renewable resources on Earth, an important feedstock for the supply of polymers and chemicals and an alternative to the use of petroleum (Guerriero et al., 2014a, 2015a). It is mainly composed of secondary cell walls, heterogeneous composites of polysaccharides (cellulose, hemicelluloses) and proteins impregnated with the aromatic polymer lignin, which confers mechanical support and recalcitrance to degradation (Guerriero et al., 2015b). Cell walls are dynamic structures which are continuously synthesized and remodeled during plant development to accommodate the needs of the growing plant cells and the broad variety of cell shapes (Guerriero et al., 2014b). The biosynthesis of plant cell walls is a complex and highly regulated process and several aspects have been thoroughly investigated. This has been possible thanks to the large amount of data generated via transcriptomics, proteomics, metabolomics on both woody and herbaceous species (Lin et al., 2013; Printz et al., 2015) and to functional studies in model plants, i.e., Arabidopsis thaliana (Anderson et al., 2015). The increasing number of sequenced plant genomes and genome-wide analyses of cellulose and lignin-related genes (Peng et al., 2013; Myburg et al., 2014) has further contributed to our understanding of plant cell wall biosynthesis. Moreover, the availability of plant cell culture systems differentiating tracheary elements (Oda et al., 2005) has provided a valuable tool to study the sequential steps of secondary cell wall biosynthesis using high-resolution imaging techniques (Lacayo et al., 2010).
Plant cell wall biosynthesis is regulated during different stages of gene expression, namely at the transcriptional and post-translational level (Hijazi et al., 2014; Zhong and Ye, 2014). A transcriptional wiring composed of master and downstream regulators determines the regulation of cell wall structural genes, typically encoding cellulose synthases and other carbohydrate-active enzymes, or enzymes in lignin biosynthesis. This hierarchical organization is conserved across different species, from woody to herbaceous, from monocots to dicots (Winzell et al., 2010; Zhao and Bartley, 2014).
At the post-translational level, plant wall biosynthesis requires the assembly of large protein complexes at the membranes, a process which relies upon intensive intracellular trafficking (Wightman and Turner, 2010) and cytoskeleton interactions (Gutierrez et al., 2009). A key example entails the cellulose synthase complex (CSC, a.k.a the “rosette terminal complex”) which is pre-assembled in the Golgi and then delivered in vesicles to the plasma membrane (Wightman and Turner, 2010). The complex was recently shown to be composed of six particles, each containing a trimer of cellulose synthase catalytic subunits (CESA4, CESA7, CESA8) in equimolar stoichiometry (1:1:1), in A. thaliana secondary cell walls (Hill et al., 2014). CSC establishes interactions with other proteins during its vesicle trafficking to and from the membrane (Gutierrez et al., 2009) and at the plasma membrane (Vain et al., 2014). Protein–protein interactions are likewise crucial for the biosynthesis of non-cellulosic polysaccharides in the Golgi (reviewed by Oikawa et al., 2013) and for the organization of multienzyme complexes, or “metabolons” (Jørgensen et al., 2005), which maximize shunting of metabolites into branches of the plant secondary metabolism that are relevant to the cell wall, such as the phenylpropanoid pathway (Chen et al., 2014).
It is therefore clear that the formation of supramolecular protein complexes is crucial for cell wall biosynthesis in plants, and in this perspective we aim at understanding more about the scaffolding components mediating protein–protein interaction, to inspire additional biotechnological strategies to tailor plant cell wall biosynthesis. We focus on a widely distributed family of scaffolding proteins, the WD40-repeat proteins (WDRs), and discuss their role in cell wall biosynthesis by presenting evidence linking these proteins to cell wall-related processes. We also present potential biotechnological uses of plant WDRs to modulate lignocellulose synthesis.
Plant WDR Proteins Have Pleiotropic Functions
Plants are among the most complicated organisms to study from a systems biology perspective. Their metabolic redundancy, a consequence of their sessile lifestyle (Mishra et al., 2012), determines intricate interactome maps (Morsy et al., 2008). Protein–protein interactions coordinate the formation of supramolecular complexes which ensure the correct execution of sequential steps in a specific metabolic pathway. The association of proteins, either temporal or stable, requires usually the presence of ancillary scaffolding proteins. In eukaryotes a family of scaffolding proteins, the WDR proteins, participates in assembling protein complexes (Stirnimann et al., 2010). The structure of these proteins is a β-propeller, where several repeating units, composed of ca 40–60 amino acids (among which conserved GH and WD residues), fold into four-stranded anti-parallel β-sheets (Stirnimann et al., 2010; Mishra et al., 2012). The β-sheets arrange circularly around a central axis, thereby creating a multi-bladed toroidal structure which can establish molecular interactions via the top, bottom, and circumference (Figures 1A,B). WDR proteins are rigid platforms that can interact with many partners, a phenomenon known as “moonlighting.” This promiscuity explains the wide range of cellular processes in which they are implicated (Stirnimann et al., 2010; Mishra et al., 2012), which span from (a)biotic stress response to intracellular trafficking and transcriptional regulation, just to name a few.
FIGURE 1. Cell wall-related WDRs: predicted 3D structure, conserved genomic neighbors of CesAs, and model of their possible roles. (A) Predicted 3D structure of AtWDR83 (AT5G64730), view from the top of the propeller, showing the seven blades; (B) view from the side, showing the toroidal structure with top, bottom and circumferential sites of interactions; (C) View of predicted 3D structure of TWD40-2 (AT5G24710), showing the two seven-bladed propellers; The models were obtained with Phyre2 (Kelley et al., 2015; available at http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index) and visualized with Swiss-PdbViewer (Guex and Peitsch, 1997; available at http://www.expasy.org/spdbv/). Templates for the AtWDR83 model were multiple WDR proteins, including Gbeta5/RGS9, ribosomal Rsa4, and pre-mRNA-splicing factor prp5, whereas the TWD40-2 template was the coatomer subunit alpha, alpha-COP; (D) Conserved microcolinearity of WDR and CesA genes in angiosperms. Unch, Uncharacterized; EamA, EamA-like transporter; LRR, Leucine-rich repeat; (E) Hypothetical model showing the possible functions of WDR53/WDR83-like proteins in cellulose biosynthesis. 1: WDR53/WDR83 represent a “platform” for the binding of unknown cell wall-related proteins; 2: WDR53/WDR83 interact with the spliceosome and splices CesA transcripts; 3: WDR53/WDR83 interact with the cytoskeleton; 4: WDR53/WDR83 bind ubiquitin; 5: WDR53/WDR83 mediate trafficking of Golgi vesicles (v); 6: WDR53/WDR83 interact with CESA complexes at the plasma membrane.
Plant WDR Proteins with Roles in Cell Wall-Related Processes
Given their functional versatility and scaffolding properties, WDR proteins are plausible candidates for roles in cell wall biosynthesis, although to date only three WDR proteins have been associated to the cell wall through experimental evidence, namely FRAGILE FIBER3 (FRA3), LEUNIG Homolog (LUH) and TWD40-2. In Arabidopsis, FRA3 is a type II inositol polyphosphate 5-phosphatases containing an N-terminal WDR domain (Zhong et al., 2004). Mutations in the FRA3 gene cause phenotypes related to a reduced secondary cell wall thickness in fibers and xylem vessels, with consequent decrease in stem strength (Zhong et al., 2004). Analysis of cell wall composition showed that the mutants have a 37% reduction in glucose, thereby suggesting that cellulose is mainly affected by the mutations (Zhong et al., 2004). Moreover, the actin organization in fiber cells is compromised with consequences on trafficking of vesicles containing CESA complexes and/or cell wall polysaccharides. The second Arabidopsis WDR protein, LUH, is a transcriptional co-repressor belonging to the Gro/TLE family (Bui et al., 2011). Arabidopsis luh-1 mutants have defects associated with mucilage extrusion, which are reminiscent of the mum2 mutants (mucilage modified 2, a β-galactosidase involved in mucilage maturation). LUH and (possibly) the closely related WDR protein LEUNIG (LUG) regulate the expression of cell wall-related genes associated with mucilage maturation (Bui et al., 2011). The penetrance of the luh-1 phenotype indicates that this WDR protein is a key regulator of cell wall modifications associated with a critical phase of seed coat maturation (Bui et al., 2011). The third Arabidopsis WDR protein, TWD40-2, was recently linked to clathrin-mediated endocytosis of cellulose synthases during cellulose biosynthesis (Bashline et al., 2015). Mutation of the TWD40-2 gene caused impaired endocytosis, unregulated overaccumulation of CESAs at the plasma membrane, decreased cellulose content and stunted plant growth. This highlights the importance of controlled CESA endocytosis in regulating cellulose biosynthesis, and the TWD40-2 WDR protein is key in this process. TWD40-2 is unique to plants and some protozoan species, and is similar to the alpha and beta coatomer WDR subunits, alpha- and beta-COP (Zhang et al., 2015). Although TWD40-2 is structurally related to alpha- and beta-COP, with its two N-terminal propellers and a C-terminal alpha-helical region (Figure 1C), it is unclear whether it coats endocytic vesicles as alpha- and beta-COP do (Dodonova et al., 2015).
WDR Proteins Linked to the Cell Wall by In Silico Predictions
Although only three WDR proteins have been linked to the cell wall by biochemical and/or genetic evidence, several potential cell wall-related WDR proteins were recently identified using in silico approaches, such as analyses of co-expression, interactome and conserved gene neighborhood. A WDR gene (At5g53500) was found to be highly co-regulated with the primary cell wall CesA1, CesA3, and CesA6 by regression analysis of public microarray data sets (Persson et al., 2005). More recently, a computational framework was developed for the prediction of proteins related to cell wall synthesis, based on known cell wall synthesis-related proteins and their predicted protein-protein interactions in the Arabidopsis interactome, which is inferred from multiple indirect lines of evidence, including coexpression, colocalization, coevolution, annotation similarity, domain interaction, and homologous interactions in other species (Geisler-Lee et al., 2007; Zhou et al., 2010). The study identified three WDR proteins, AT1G04510, AT2G43770, and AT4G34460, among the 42 most likely cell wall-related candidate proteins. Other studies show that AT1G04510 codes for MAC3A, a WDR protein with roles in plant immunity and activation of the spliceosome (Monaghan et al., 2009). The computational analysis predicts that MAC3A/AT1G04510 interacts with a rhamnose synthase, RHM1 (AT1G78570), involved in accumulation of the pectic polysaccharide rhamnogalacturonan in the cell wall. Both functions of MAC3A, spliceosome activation and plant defense, are consistent with a possible role in the cell wall, as both processes may entail the cell wall (Huang et al., 2013; Bellincampi et al., 2014). Further, cell wall pectin is an important factor in plant defense (Bellincampi et al., 2014), and thus the predicted interaction of MAC3A/AT1G04510 with RHM1 is in agreement with its function. Then, MAC3A/AT1G04510 could potentially regulate plant defense through splicing of the RHM1/AT1G78570 transcript, in analogy to regulation by splicing of Callose Synthase5 (Huang et al., 2013), although this remains to be shown experimentally. The second predicted cell wall-related WDR protein, AT2G43770, is the plant homolog of SNRNP40, also a spliceosome component, and is predicted to interact with a UDP-D-xylose synthase, UXS6 (AT2G28760). Finally, the third predicted cell wall WDR protein, AT4G34460, is AGB1, a G-Protein β subunit, and the study predicts its interaction with a GDP-D-mannose 3′,5′-epimerase (AT5G28840). The role of AGB1 in the cell wall was experimentally confirmed in later studies showing its involvement in cell wall defense (Delgado-Cerezo et al., 2012), suggesting a fair level of accurate predictions of cell wall-involved WDRs by the computational framework.
Conserved Collinear Microsynteny between WDR and Glycan Synthase Genes in Diverse Eukaryote Taxa
The clustering of functionally related genes is prevalent in prokaryotes but was conventionally assumed to be less common in eukaryotes. However, this notion is shifting as clusters of functionally related genes are increasingly detected in eukaryotes (Hurst et al., 2004; Yi et al., 2007) including, recently, plants (Nützmann and Osbourn, 2014). Although gene clusters in plant and fungi concern secondary metabolism, a recent bioinformatic study identified a cell wall gene cluster in fungi (Pacheco-Arjona and Ramirez-Prado, 2014), suggesting that conserved genomic neighborhood may provide a means to identifying genes involved in processes other than secondary metabolism. The function of physically clustering functionally related genes in eukaryotes is unclear, but could involve coordinated gene expression or keeping genes together to avoid toxic effects of their individual loss.
We previously identified two cases of conserved genomic collinearity between a WDR gene and a CesA in the genomes of several dicots (Guerriero et al., 2012). Specifically, a plant WDR53 gene (represented by At5g45760 in Arabidopsis) is collinear with the secondary cell wall CesA8, while a WDR83 (At5g64730) is adjacent to the primary cell wall CesA6 (schematized in Figure 1D). We showed that WDR53 and CesA8 are partially co-expressed in apple and Populus (Guerriero et al., 2012). Further, we identified a bicistronic WDR53-CesA8 transcript in phytoplasma-infected apple tissues, where the CesA8 cistron is alternatively spliced and lacks a portion corresponding the RING-type zinc finger domain. We speculate on whether this alternatively spliced CesA8 cistron is translated in order to alter cellulose biosynthesis in response to phytoplasma infection.
We have also showed that the genomic association between a WDR gene and a gene encoding a glycosyltransferase from family 2 (GT2) is a conserved feature in eukaryotes, since, besides dicots, it is reported in metazoans (hyaluronan synthase-WDR microsynteny) and in fungi (chitin synthase-WDR microsynteny; Guerriero et al., 2012). In fungi, the genomic collinearity between a chitin synthase (chs) and a WDR gene is conserved among several species (Guerriero et al., in revision). This WDR gene (AN10216 in Aspergillus nidulans) occurs mainly in fungi and plants, and is therefore named Fungal-Plant WDR (FPWD). FPWD is localized within a recently identified cell wall metabolism gene cluster involving among others, besides chsD, a Chs activator, a myosin V, a GH17 cell wall glucanase, scw11 (Pacheco-Arjona and Ramirez-Prado, 2014). Deletion of FPWD and a tightly linked neighboring beta-flanking gene causes cell wall and chitin-related phenotypes in A. nidulans, confirming FPWD’s role in the cell wall (Guerriero et al., in revision). These results suggest that identifying conserved gene neighbors may be a useful bioinformatic approach to mining for gene targets, in addition to more traditional approaches based on gene expression, proteomics or interactome inference.
In light of the conserved genomic microsynteny WDR53-CesA8 and WDR83-CesA6, it is then plausible to assume a cellulose biosynthesis-related role for these WD40 proteins. Given the moonlighting properties of many plant WDR proteins, they could be involved in one or several processes involving the delivery of the CSC to membrane microdomains, e.g., via regulating transcript splicing, vesicle trafficking, the formation of platforms mediating protein-protein interactions and/or protein turnover via ubiquitination (schematized in Figure 1E).
In flowering plants, the FPWD gene occurs as a small gene family of around five genes of unknown function, represented in Arabidopsis by At1g78070, At1g36070, At1g55680, At3g13340, and At5g56190. We noticed that the plant FPWDs are conserved genomic neighbors of cell wall or carbohydrate-related genes, coding for trehalose-6-phosphate phosphatase, GH3 and actin ACT7 (in higher plants), as well as nucleotide sugar transporter and GT57 glucosyltransferase (in green algae). Examination of their expression using publicly available microarray databases in Arabidopsis and Populus suggests that they may be either largely xylem-specific, or pollen-specific (data not shown), consistent with a possible role in the cell wall. Clearly, the precise functions of these potentially wall-involved WDRs, and of their gene neighbors, remain to be further elucidated.
Phylogenetic Relationships of Cell Wall Candidate WDR Proteins
We here identify a number of plant WDR proteins with confirmed or potential roles in the lignocellulosic cell wall. A recent phylogenetic analysis of all Arabidopsis (comprising 237 gene ids) and rice (comprising 200 gene ids) WDRs established that the plant WDR phylogenetic tree consists of five major branches, numbered I–V (Ouyang et al., 2012). To better understand the function and evolution of the cell wall candidate WDRs, we analyzed their cluster location within the phylogenetic tree (Table 1). We observe that cell wall-related WDR proteins belong to distinct phylogenetic clusters: WDR83, MAC3A, AT2G43770 and AGB1 belong to cluster I, together with WDR5, involved in histone methylation (Jiang et al., 2011). WDR53 is in cluster III with FRA3, involved in secondary cell wall formation, ROOT INITIATION DEFECTIVE 3, RID3, with roles in pre-rRNA processing (Shinohara et al., 2014), and SPIRRIG, a BEACH domain WDR which regulates mRNA stability (Steffens et al., 2015). Further, the FPWDs branch in cluster II, together with the TOPLESS-related, TPR, protein cluster, which are involved in chromatin-mediated repression by histone deacetylation (Pi et al., 2015), and RBBP5 LIKE, RBL, involved in histone methylation (Jiang et al., 2011). Finally, TWD40-2, involved in clathrin mediated endocytosis of CESA complexes, also branches in cluster II. In summary, the cell wall candidate WDR proteins do not cluster together but are rather widely distributed across the plant WDR superfamily.
TABLE 1. Phylogenetic cluster location of cell wall-related WDR proteins (upper section), listed together with representative members of each cluster (lower section).
Biotechnological Prospects: WDR Proteins to Boost Plant Biomass Production
The depletion of fossil fuel pushes toward the identification and use of renewable energy sources to promote the development of a circular economy. In this perspective plant biomass is very important, since it constitutes one of the most abundant sustainable resources for humanity and it can provide biopolymers, fibers, biofuel, thereby promoting economic growth, while minimizing environmental impacts. Besides woody species like Populus and Eucalyptus, fast growing herbaceous species, like the fiber-crops flax and hemp, have also considerable importance for the bio-economy, because of their shorter growth cycles, their high biomass yield (e.g., 24 tons/hectare for hemp; Kolaříková et al., 2013) and the presence of cellulosic fibers in their stems (the so-called bast fibers; Guerriero et al., 2013). Because of these advantageous characteristics and the properties of their fibers (length, strength, renewability), these crops are widely used in industry to provide fibers for the reinforcement of composites. For the bio-economy high biomass-production is a sought-after character and biotechnological strategies aimed at boosting biomass formation are attracting research efforts (Nieminen et al., 2012; Dubouzet et al., 2013). Here we identify a list of WDR proteins with confirmed or potential roles in the lignocellulosic cell wall, which are interesting targets for engineering plants with enhanced biomass production. Such effort would involve the use of promoters to drive altered expression in the cambium or xylem (Ji et al., 2010; Ratke et al., 2015), to influence xylem proliferation and/or lignocellulose biosynthesis. As one possible example, seedlings of twd40-2 mutants display reduced endocytosis, cellulose content, and plant growth. The TWD40-2 gene is expressed in most tissues, but its expression is highest in stem or xylem in Arabidopsis and Populus according to the BAR eFT gene expression browser (http://bar.utoronto.ca/; data not shown). Then, enhancing TWD40-2 expression in the xylem could increase cellulose content. A further approach to enhance the function of target WDR proteins could potentially involve mutation of selected surface residues involved in protein interaction, a.k.a. “interaction hotspots,” in order to decrease the target’s eventual pleiotropy. This would require identification of interaction hotspots for example using tools for in silico prediction, such as modeling their 3D structure, combined with experimental confirmation. The ultimate goal of such mutagenesis, which could be generated through genome editing, would be to reduce pleiotropy or moonlighting of the target WDR, and obtain more specialized enzymes, to avoid undesired side effects.
Author Contributions
GG and IE conceived the idea of writing the Perspective paper. GG and IE obtained the bioinformatic data. GG, J-FH, and IE interpreted the data and wrote the manuscript.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
GG gratefully acknowledges the Austrian Science Fund (FWF; http://www.fwf.ac.at/en/) Grant No. M1315. GG and JFH wish to thank the support by the Fonds National de la Recherche, Luxembourg, (Project CANCAN C13/SR/5774202). IE gratefully acknowledges the Swedish Governmental Agency for Innovation Systems, VINNOVA and the Swedish Energy Agency, Energimyndigheten for financial support.
References
Anderson, N., Bonawitz, N. D., Nyffeler, K. E., and Chapple, C. (2015). Loss of ferulate 5-hydroxylase leads to Mediator-dependent inhibition of soluble phenylpropanoid biosynthesis in Arabidopsis. Plant Physiol. 169, 1557–1567. doi: 10.1104/pp.15.00294
Bashline, L., Li, S., Zhu, X., and Gu, Y. (2015). The TWD40-2 protein and the AP2 complex cooperate in the clathrin-mediated endocytosis of cellulose synthase to regulate cellulose biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 112, 12870–12875. doi: 10.1073/pnas.1509292112
Bellincampi, D., Cervone, F., and Lionetti, V. (2014). Plant cell wall dynamics and wall-related susceptibility in plant–pathogen interactions. Front. Plant Sci. 5:228. doi: 10.3389/fpls.2014.00228
Bui, M., Lim, N., Sijacic, P., and Liu, Z. (2011). LEUNIG_HOMOLOG and LEUNIG regulate seed mucilage extrusion in Arabidopsis. J. Integr. Plant Biol. 53, 399–408. doi: 10.1111/j.1744-7909.2011.01036.x
Chen, H. C., Song, J., Williams, C. M., Shuford, C. M., Liu, J., Wang, J. P., et al. (2014). Systems biology of lignin biosynthesis in Populus trichocarpa: heteromeric 4-coumaric acid:coenzyme A ligase protein complex formation, regulation, and numerical modeling. Plant Cell 26, 876–893. doi: 10.1105/tpc.113.119685
Delgado-Cerezo, M., Sánchez-Rodríguez, C., Escudero, V., Miedes, E., Fernández, P. V., Jordá, L., et al. (2012). Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi. Mol. Plant 5, 98–114. doi: 10.1093/mp/ssr082
Dodonova, S. O., Diestelkoetter-Bachert, P., von Appen, A., Hagen, W. J., Beck, R., Beck, M., et al. (2015). VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science 349, 195–198. doi: 10.1126/science.aab1121
Dubouzet, J. G., Strabala, T. J., and Wagner, A. (2013). Potential transgenic routes to increase tree biomass. Plant Sci. 212, 72–101. doi: 10.1016/j.plantsci.2013.08.006
Geisler-Lee, J., O’Toole, N., Ammar, R., Provart, N. J., Millar, A. H., and Geisler, M. (2007). A predicted interactome for Arabidopsis. Plant Physiol. 145, 317–329. doi: 10.1104/pp.107.103465
Guerriero, G., Hausman, J. F., Strauss, J., Ertan, H., and Siddiqui, K. S. (2015a). Lignocellulosic biomass: biosynthesis, degradation and industrial utilization. Eng. Life Sci. doi: 10.1002/elsc.201400196
Guerriero, G., Hausman, J. F., Strauss, J., Ertan, H., and Siddiqui, K. S. (2015b). Destructuring plant biomass: focus on fungal and extremophilic cell wall hydrolases. Plant Sci. 234, 180–193. doi: 10.1016/j.plantsci.2015.02.010
Guerriero, G., Sergeant, K., and Hausman, J. F. (2013). Integrated -omics: a powerful approach to understanding the heterogeneous lignification of fibre crops. Int. J. Mol. Sci. 14, 10958–10978. doi: 10.3390/ijms140610958
Guerriero, G., Sergeant, K., and Hausman, J. F. (2014a). Wood biosynthesis and typologies: a molecular rhapsody. Tree Physiol. 34, 839–855. doi: 10.1093/treephys/tpu031
Guerriero, G., Hausman, J. F., and Cai, G. (2014b). No stress! Relax! Mechanisms governing growth and shape in plant cells. Int. J. Mol. Sci. 15, 5094–5114. doi: 10.3390/ijms15035094
Guerriero, G., Spadiut, O., Kerschbamer, C., Giorno, F., Baric, S., and Ezcurra, I. (2012). Analysis of cellulose synthase genes from domesticated apple identifies collinear genes WDR53 and CesA8A: partial co-expression, bicistronic mRNA, and alternative splicing of CESA8A. J. Exp. Bot. 63, 6045–6056. doi: 10.1093/jxb/ers255
Guex, N., and Peitsch, M. C. (1997). SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis 18, 2714–2723. doi: 10.1002/elps.1150181505
Gutierrez, R., Lindeboom, J. J., Paredez, A. R., Emons, A. M., and Ehrhardt, D. W. (2009). Arabidopsis cortical microtubules position cellulose synthase delivery to the plasma membrane and interact with cellulose synthase trafficking compartments. Nat. Cell Biol. 11, 797–806. doi: 10.1038/ncb1886
Hijazi, M., Velasquez, S. M., Jamet, E., Estevez, J. M., and Albenne, C. (2014). An update on post-translational modifications of hydroxyproline-rich glycoproteins: toward a model highlighting their contribution to plant cell wall architecture. Front. Plant Sci. 5:395. doi: 10.3389/fpls.2014.00395
Hill, J. L. Jr., Hammudi, M. B., and Tien, M. (2014). The Arabidopsis cellulose synthase complex: a proposed hexamer of CESA trimers in an equimolar stoichiometry. Plant Cell 26, 4834–4842. doi: 10.1105/tpc.114.131193
Huang, X. Y., Niu, J., Sun, M. X., Zhu, J., Gao, J. F., Yang, J., et al. (2013). CYCLIN-DEPENDENT KINASE G1 is associated with the spliceosome to regulate CALLOSE SYNTHASE5 splicing and pollen wall formation in Arabidopsis. Plant Cell 25, 637–648. doi: 10.1105/tpc.112.107896
Hurst, L. D., Pál, C., and Lercher, M. J. (2004). The evolutionary dynamics of eukaryotic gene order. Nat. Rev. Genet. 5, 5299–5310. doi: 10.1038/nrg1319
Ji, J., Strable, J., Shimizu, R., Koenig, D., Sinha, N., and Scanlon, M. J. (2010). WOX4 promotes procambial development. Plant Physiol. 152, 1346–1356. doi: 10.1104/pp.109.149641
Jiang, D., Kong, N. C., Gu, X., Li, Z., and He, Y. (2011). Arabidopsis COMPASS-like complexes mediate histone H3 lysine-4 trimethylation to control floral transition and plant development. PLoS Genet. 7:e1001330. doi: 10.1371/journal.pgen.1001330
Jørgensen, K., Rasmussen, A. V., Morant, M., Nielsen, A. H., Bjarnholt, N., Zagrobelny, M., et al. (2005). Metabolon formation and metabolic channeling in the biosynthesis of plant natural products. Curr. Opin. Plant Biol. 8, 280–291. doi: 10.1016/j.pbi.2005.03.014
Kelley, L. A., Mezulis, S., Yates, C. M., Wass, M. N., and Sternberg, M. J. (2015). The Phyre2 web portal for protein modeling, prediction and analysis. Nat. Protoc. 10, 845–858. doi: 10.1038/nprot.2015.053
Kolaříková, M., Ivanova, T., and Havrland, B. (2013). “Energy balance of briquettes made of hemp (Cannabis sativa L.) cultivars (Ferimon, Bialobrzeskie) from autumn harvest to produce heat for household use,” in Proceedings of the Engineering for Rural Development (Jelgava: Latvia University of Agriculture), 504–508.
Lacayo, C. I., Malkin, A. J., Holman, H. Y., Chen, L., Ding, S. Y., Hwang, M. S., et al. (2010). Imaging cell wall architecture in single Zinnia elegans tracheary elements. Plant Physiol. 154, 121–133. doi: 10.1104/pp.110.155242
Lin, Y. C., Li, W., Sun, Y. H., Kumari, S., Wei, H., Li, Q., et al. (2013). SND1 transcription factor-directed quantitative functional hierarchical genetic regulatory network in wood formation in Populus trichocarpa. Plant Cell 25, 4324–4341. doi: 10.1105/tpc.113.117697
Mishra, A. K., Puranik, S., and Prasad, M. (2012). Structure and regulatory networks of WD40 protein in plants. J. Plant Biochem. Biotechnol. 21, S32–S39. doi: 10.1111/j.1744-7909.2012.01159.x
Monaghan, J., Xu, F., Gao, M., Zhao, Q., Palma, K., Long, C., et al. (2009). Two Prp19-like U-box proteins in the MOS4-associated complex play redundant roles in plant innate immunity. PLoS Pathog. 5:e1000526. doi: 10.1371/journal.ppat.1000526
Morsy, M., Gouthu, S., Orchard, S., Thorneycroft, D., Harper, J. F., Mittler, R., et al. (2008). Charting plant interactomes: possibilities and challenges. Trends Plant Sci. 13, 183–191. doi: 10.1016/j.tplants.2008.01.006
Myburg, A. A., Grattapaglia, D., Tuskan, G. A., Hellsten, U., Hayes, R. D., Grimwood, J., et al. (2014). The genome of Eucalyptus grandis. Nature 510, 356–362. doi: 10.1038/nature13308
Nieminen, K., Robischon, M., Immanen, J., and Helariutta, Y. (2012). Towards optimizing wood development in bioenergy trees. New Phytol. 194, 46–53. doi: 10.1111/j.1469-8137.2011.04011.x
Nützmann, H. W., and Osbourn, A. (2014). Gene clustering in plant specialized metabolism. Curr. Opin. Biotechnol. 26, 91–99. doi: 10.1016/j.copbio.2013.10.009
Oda, Y., Mimura, T., and Hasezawa, S. (2005). Regulation of secondary cell wall development by cortical microtubules during tracheary element differentiation in Arabidopsis cell suspensions. Plant Physiol. 137, 1027–1036. doi: 10.1104/pp.104.052613
Oikawa, A., Lund, C. H., Sakuragi, Y., and Scheller, H. V. (2013). Golgi-localized enzyme complexes for plant cell wall biosynthesis. Trends Plant Sci. 18, 49–58. doi: 10.1016/j.tplants.2012.07.002
Ouyang, Y., Huang, X., Lu, Z., and Yao, J. (2012). Genomic survey, expression profile and co-expression network analysis of OsWD40 family in rice. BMC Genomics 20:100. doi: 10.1186/1471-2164-13-100
Pacheco-Arjona, J. R., and Ramirez-Prado, J. H. (2014). Large-scale phylogenetic classification of fungal chitin synthases and identification of a putative cell-wall metabolism gene cluster in Aspergillus genomes. PLoS ONE 9:e104920. doi: 10.1371/journal.pone.0104920
Peng, Z., Lu, Y., Li, L., Zhao, Q., Feng, Q., Gao, Z., et al. (2013). The draft genome of the fast-growing non-timber forest species moso bamboo (Phyllostachys heterocycla). Nat. Genet. 45, 456–461. doi: 10.1038/ng.2569
Persson, S., Wei, H., Milne, J., Page, G. P., and Somerville, C. R. (2005). Identification of genes required for cellulose synthesis by regression analysis of public microarray data sets. Proc. Natl. Acad. Sci. U.S.A. 102, 8633–8638. doi: 10.1073/pnas.0503392102
Pi, L., Aichinger, E., van der Graaff, E., Llavata-Peris, C. I., Weijers, D., Hennig, L., et al. (2015). Organizer-derived WOX5 signal maintains root columella stem cells through chromatin-mediated repression of CDF4 expression. Dev. Cell. 33, 576–588. doi: 10.1016/j.devcel.2015.04.024
Printz, B., Guerriero, G., Sergeant, K., Renaut, J., Lutts, S., and Hausman, J. F. (2015). Ups and downs in alfalfa: proteomic and metabolic changes occurring in the growing stem. Plant Sci. 238, 13–25. doi: 10.1016/j.plantsci.2015.05.014
Ratke, C., Pawar, P. M., Balasubramanian, V. K., Naumann, M., Duncranz, M. L., Derba-Maceluch, M., et al. (2015). Populus GT43 family members group into distinct sets required for primary and secondary wall xylan biosynthesis and include useful promoters for wood modification. Plant Biotechnol. J. 13, 26–37. doi: 10.1111/pbi.12232
Shinohara, N., Ohbayashi, I., and Sugiyama, M. (2014). Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration. Front. Plant Sci. 5:159. doi: 10.3389/fpls.2014.00159
Steffens, A., Bräutigam, A., Jakoby, M., and Hülskamp, M. (2015). The BEACH domain protein SPIRRIG is essential for Arabidopsis salt stress tolerance and functions as a regulator of transcript stabilization and localization. PLoS Biol. 13:e1002188. doi: 10.1371/journal.pbio.1002188
Stirnimann, C. U., Petsalaki, E., Russell, R. B., and Müller, C. W. (2010). WD40 proteins propel cellular networks. Trends Biochem. Sci. 35, 565–574. doi: 10.1016/j.tibs.2010.04.003
Vain, T., Crowell, E. F., Timpano, H., Biot, E., Desprez, T., Mansoori, N., et al. (2014). The cellulase KORRIGAN is part of the cellulose synthase complex. Plant Physiol. 165, 1521–1532. doi: 10.1104/pp.114.241216
Wightman, R., and Turner, S. (2010). Trafficking of the plant cellulose synthase complex. Plant Physiol. 153, 427–432. doi: 10.1093/aob/mcu040
Winzell, A., Aspeborg, H., Wang, Y., and Ezcurra, I. (2010). Conserved CA-rich motifs in gene promoters of Pt x tMYB021-responsive secondary cell wall carbohydrate-active enzymes in Populus. Biochem. Biophys. Res. Commun. 394, 848–853. doi: 10.1016/j.bbrc.2010.03.10
Yi, G., Sze, S. H., and Thon, M. R. (2007). Identifying clusters of functionally related genes in genomes. Bioinformatics 23, 1053–1060. doi: 10.1093/bioinformatics/btl673
Zhang, Y., Persson, S., Hirst, J., Robinson, M. S., van Damme, D., and Sánchez-Rodríguez, C. (2015). Change your TPLATE, change your fate: plant CME and beyond. Trends Plant Sci. 20, 41–48. doi: 10.1016/j.tplants.2014.09.002
Zhao, K., and Bartley, L. E. (2014). Comparative genomic analysis of the R2R3 MYB secondary cell wall regulators of Arabidopsis, poplar, rice, maize, and switchgrass. BMC Plant Biol. 14:135. doi: 10.1186/1471-2229-14-135
Zhong, R., Burk, D. H., Morrison, W. H. III, and Ye, Z. H. (2004). FRAGILE FIBER3, an Arabidopsis gene encoding a type II inositol polyphosphate 5-phosphatase, is required for secondary wall synthesis and actin organization in fiber cells. Plant Cell 16, 3242–3259. doi: 10.1105/tpc.104.027466
Zhong, R., and Ye, Z. H. (2014). Complexity of the transcriptional network controlling secondary wall biosynthesis. Plant Sci. 229, 193–207. doi: 10.1016/j.plantsci.2014.09.009
Keywords: WDR proteins, protein–protein interaction, plant cell wall, genomic collinearity, lignocellulose
Citation: Guerriero G, Hausman J-F and Ezcurra I (2015) WD40-Repeat Proteins in Plant Cell Wall Formation: Current Evidence and Research Prospects. Front. Plant Sci. 6:1112. doi: 10.3389/fpls.2015.01112
Received: 29 September 2015; Accepted: 24 November 2015;
Published: 22 December 2015.
Edited by:
Hemayet Ullah, Howard University, USAReviewed by:
Ian S. Wallace, The University of Nevada, Reno, USABiswapriya Biswavas Misra, University of Florida, USA
Copyright © 2015 Guerriero, Hausman and Ezcurra. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Inés Ezcurra, aW5lc0BiaW90ZWNoLmt0aC5zZQ==