AUTHOR=Wang Yongli , Wang Hui , Ma Yujie , Du Haiping , Yang Qing , Yu Deyue 

TITLE=Identification of transcriptional regulatory nodes in soybean defense networks using transient co-transactivation assays

JOURNAL=Frontiers in Plant Science

VOLUME=6

YEAR=2015

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2015.00915

DOI=10.3389/fpls.2015.00915

ISSN=1664-462X

ABSTRACT=<p>Plant responses to major environmental stressors, such as insect feeding, not only occur via the functions of defense genes but also involve a series of regulatory factors. Our previous transcriptome studies proposed that, in addition to two defense-related genes, <italic>GmVSP</italic>β and <italic>GmN:IFR</italic>, a high proportion of transcription factors (TFs) participate in the incompatible soybean-common cutworm interaction networks. However, the regulatory mechanisms and effects of these TFs on those induced defense-related genes remain unknown. In the present work, we isolated and identified 12 genes encoding MYB, WRKY, NAC, bZIP, and DREB TFs from a common cutworm-induced cDNA library of a resistant soybean line. Sequence analysis of the promoters of three co-expressed genes, including <italic>GmVSP</italic>α, <italic>GmVSP</italic>β, and <italic>GmN:IFR</italic>, revealed the enrichment of various TF-binding sites for defense and stress responses. To further identify the regulatory nodes composed of these TFs and defense gene promoters, we performed extensive transient co-transactivation assays to directly test the transcriptional activity of the 12 TFs binding at different levels to the three co-expressed gene promoters. The results showed that all 12 TFs were able to transactivate the <italic>GmVSP</italic>β and <italic>GmN:IFR</italic> promoters. <italic>GmbZIP110</italic> and <italic>GmMYB75</italic> functioned as distinct regulators of <italic>GmVSP</italic>α/β and <italic>GmN:IFR</italic> expression, respectively, while <italic>GmWRKY39</italic> acted as a common central regulator of <italic>GmVSP</italic>α/β and <italic>GmN:IFR</italic> expression. These corresponding TFs play crucial roles in coordinated plant defense regulation, which provides valuable information for understanding the molecular mechanisms involved in insect-induced transcriptional regulation in soybean. More importantly, the identified TFs and suitable promoters can be used to engineer insect-resistant plants in molecular breeding studies.</p>