AUTHOR=Perry Gregory E., DiNatale Claudia , Xie Weilong , Shi Chun , Navabi Alireza , Reinprecht Yarmilla , Crosby William C., Yu Kangfu , Pauls K. Peter 

TITLE=A comparison of the molecular organization of genomic regions associated with resistance to common bacterial blight in two Phaseolus vulgaris genotypes

JOURNAL=Frontiers in Plant Science

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2013.00318

DOI=10.3389/fpls.2013.00318

ISSN=1664-462X

ABSTRACT=<p>Resistance to common bacterial blight, <italic>caused by Xanthomonas axonopodis</italic> pv. phaseoli, in <italic>Phaseolus vulgaris</italic> is conditioned by several loci on different chromosomes. Previous studies with OAC-Rex, a CBB-resistant, white bean variety of Mesoamerican origin, identified two resistance loci associated with the molecular markers <italic>Pv</italic>-CTT001 and SU91, on chromosome 4 and 8, respectively. Resistance to CBB is assumed to be derived from an interspecific cross with <italic>Phaseolus acutifolius</italic> in the pedigree of OAC-Rex. Our current whole genome sequencing effort with OAC-Rex provided the opportunity to compare its genome in the regions associated with CBB resistance with the v1.0 release of the <italic>P. vulgaris</italic> line G19833, which is a large seeded bean of Andean origin, and (assumed to be) CBB susceptible. In addition, the genomic regions containing SAP6, a marker associated with <italic>P. vulgaris</italic>-derived CBB-resistance on chromosome 10, were compared. These analyses indicated that gene content was highly conserved between G19833 and OAC-Rex across the regions examined (&gt;80%). However, fifty-nine genes unique to OAC Rex were identified, with resistance gene homologues making up the largest category (10 genes identified). Two unique genes in OAC-Rex located within the SU91 resistance QTL have homology to <italic>P. acutifolius</italic> ESTs and may be potential sources of CBB resistance. As the genomic sequence assembly of OAC-Rex is completed, we expect that further comparisons between it and the G19833 genome will lead to a greater understanding of CBB resistance in bean.</p>