AUTHOR=Astudillo Carolina , Fernandez Andrea , Blair Matthew W., Cichy Karen A.

TITLE=The Phaseolus vulgaris ZIP gene family: identification, characterization, mapping, and gene expression

JOURNAL=Frontiers in Plant Science

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2013.00286

DOI=10.3389/fpls.2013.00286

ISSN=1664-462X

ABSTRACT=<p>Zinc is an essential mineral for humans and plants and is involved in many physiological and biochemical processes. In humans, Zn deficiency has been associated with retarded growth and reduction of immune response. In plants, Zn is an essential component of more than 300 enzymes including RNA polymerase, alkaline phosphatase, alcohol dehydrogenase, Cu/Zn superoxidase dismutase, and carbonic anhydrase. The accumulation of Zn in plants involves many genes and characterization of the role of these genes will be useful in biofortification. Here we report the identification and phlyogenetic and sequence characterization of the 23 members of the ZIP (ZRT, IRT like protein) family of metal transporters and three transcription factors of the bZIP family in <italic>Phaseolus vulgaris</italic> L. Expression patterns of seven of these genes were characterized in two bean genotypes (G19833 and DOR364) under two Zn treatments. Tissue analyzed included roots and leaves at vegetative and flowering stages, and pods at 20 days after flowering. Four of the genes, <italic>PvZIP12, PvZIP13, PvZIP16</italic>, and <italic>Pv bZIP1</italic>, showed differential expression based on tissue, Zn treatment, and/or genotype. <italic>PvZIP12</italic> and <italic>PvZIP13</italic> were both more highly expressed in G19833 than DOR364. <italic>PvZIP12</italic> was most highly expressed in vegetative leaves under the Zn (−) treatment. <italic>PvZIP16</italic> was highly expressed in leaf tissue, especially leaf tissue at flowering stage grown in the Zn (−) treatment. <italic>Pv bZIP1</italic> was most highly expressed in leaf and pod tissue. The 23 <italic>Pv</italic>ZIP genes and three bZIP genes were mapped on the DOR364 × G19833 linkage map. <italic>PvZIP12, PvZIP13</italic>, and <italic>PvZIP18, Pv bZIP2</italic>, and <italic>Pv bZIP3</italic> were located near QTLs for Zn accumulation in the seed. Based on the expression and mapping results, <italic>PvZIP12</italic> is a good candidate gene for increasing seed Zn concentration and increase understanding of the role of ZIP genes in metal uptake, distribution, and accumulation of zinc in <italic>P. vulgaris</italic>.</p>