AUTHOR=López-Millán Ana-Flor , Grusak Michael A., Abadia Anunciación , Abadía Javier 

TITLE=Iron deficiency in plants: an insight from proteomic approaches

JOURNAL=Frontiers in Plant Science

VOLUME=4

YEAR=2013

URL=https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2013.00254

DOI=10.3389/fpls.2013.00254

ISSN=1664-462X

ABSTRACT=<p>Iron (Fe) deficiency chlorosis is a major nutritional disorder for crops growing in calcareous soils, and causes decreases in vegetative growth as well as marked yield and quality losses. With the advances in mass spectrometry techniques, a substantial body of knowledge has arisen on the changes in the protein profiles of different plant parts and compartments as a result of Fe deficiency. Changes in the protein profile of thylakoids from several species have been investigated using gel-based two-dimensional electrophoresis approaches, and the same techniques have been used to investigate changes in the root proteome profiles of tomato (<italic>Solanum lycopersicum</italic>), sugar beet (<italic>Beta vulgaris</italic>), cucumber (<italic>Cucumis sativus</italic>), <italic>Medicago truncatula</italic> and a <italic>Prunus</italic> rootstock. High throughput proteomic studies have also been published using Fe-deficient <italic>Arabidopsis thaliana</italic> roots and thylakoids. This review summarizes the major conclusions derived from these “-omic” approaches with respect to metabolic changes occurring with Fe deficiency, and highlights future research directions in this field. A better understanding of the mechanisms involved in root Fe homeostasis from a holistic point of view may strengthen our ability to enhance Fe-deficiency tolerance responses in plants of agronomic interest.</p>