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BRIEF RESEARCH REPORT article

Front. Plant Physiol.
Sec. Plant Morphogenesis and Evolution
Volume 2 - 2024 | doi: 10.3389/fphgy.2024.1441158

Fine-tuning and remodeling of pectins play a key role in the maintenance of cell adhesion

Provisionally accepted
Cyril Grandjean Cyril Grandjean 1Aline Voxeur Aline Voxeur 1Salem Chabout Salem Chabout 1Francois Jobert Francois Jobert 2Laurent Gutierrez Laurent Gutierrez 2Jérôme Pelloux Jérôme Pelloux 3Gregory Mouille Gregory Mouille 1Sophie Bouton Sophie Bouton 3*
  • 1 Institute Jean-Pierre Bourgin, Université Paris-Saclay, Versailles, Île-de-France, France
  • 2 Centre de Ressources Régionales en Biologie Moléculaire (CRRBM), Université de Picardie Jules Verne, Amiens, France
  • 3 University of Picardie Jules Verne, Amiens, Picardy, France

The final, formatted version of the article will be published soon.

    Plant cell adhesion is essential for development and stress response and is mediated by the deposition of pectin-rich middle lamella between cell walls. However, the precise control mechanism of cell adhesion remains unclear. The qua2-1 and esmd1-1 mutants provide a better understanding of this process and suggest a signaling pathway triggering the loss and restoration of adhesion via cell wall modifications. This study aims at a better characterization of the production of endogenous oligogalacturonides (OGs) and cell wall structural modifications in the control of cell adhesion in Arabidopsis. From dark-grown hypocotyls of wild type, qua2-1, esmd1-1, and qua2-1/esmd1-1 mutants, we identified seven distinct endogenous OGs, that varied in their degree of polymerization and extent of substitution and in their abundance. The structure of homogalacturonans were further analyzed by enzymatic fingerprint, indicating a change in esterification patterns. Expression analysis of genes encoding pectin-modifying enzymes, including PME, PMEI, and PAE showed significant variations depending on genotypes. Gene expression levels correlated with changes in the structure of homogalacturonans and cell adhesion phenotypes. This study suggests the involvement of a feedback loop between endogenous OGs, the fine-tuning of homogalacturonans structure, and the gene expression of pectin remodeling enzymes in controlling cell adhesion.

    Keywords: Arabidopsis, Cell Wall, Cell Adhesion, pectin, oligogalacturonides (OGs)

    Received: 30 May 2024; Accepted: 26 Jul 2024.

    Copyright: © 2024 Grandjean, Voxeur, Chabout, Jobert, Gutierrez, Pelloux, Mouille and Bouton. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Sophie Bouton, University of Picardie Jules Verne, Amiens, 80025 CEDEX, Picardy, France

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