URINARY AMINO ACID METABOLOMIC PROFILING AND ITS ASSOCIATION WITH CHILDHOOD OBESITY IN PREPUBESCENT INDIVIDUALS

Mariana Doce Passadore ¹ Nayara Azinheira Nobrega Cruz ¹ Mariana Zuccherato Bocato ² Leonardo de Abreu Ferreira ³ Marcelo Yudi Icimoto ¹ Maria del Carmen Bisi Molina ⁴ JOSE GERALDO MILL ⁴ Fernando Barbosa Junior ² Dulce Elena Casarini ¹ Lilian Caroline Gonçalves Oliveira ^1*

• ¹ Federal University of São Paulo, São Paulo, Brazil
• ² School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
• ³ Mackenzie Presbyterian University, São Paulo, São Paulo, Brazil
• ⁴ Federal University of Espirito Santo, Vitória, Espirito Santo, Brazil

Amino acids are fundamental in several metabolic processes, and their levels can reflect metabolism impairments that contribute to obesity and related diseases. Our objective was to identify a urinary amino acid fingerprint in obese and overweight children in prepuberty and to correlate this profile with cardiometabolic alterations.The study included 110 children, boys and girls aged 9 to 10 years, they were classified according to their BMI-for-age (Body Mass Index for age) into three groups: normal weight (NW) (n=45), overweight (OW) (n=21), and obese (OB) (n=44). The 12-hour urine samples were analyzed by LC-MS/MS to quantify 47 amino acids using the Amino Acids Analysis Kit (Zivak®, Turkey), values were corrected by creatinine concentration. Anthropometric measurements, cardiovascular parameters, and biochemical profiles were assessed following standard protocols. When compared to NW, anthropometric measures, systolic and diastolic blood pressure, and serum uric acid levels were progressively elevated in the OW and OB groups. The OB group was characterized by elevated alpha-aminoadipic acid, asparagine, cystathionine, 1-methyl-histidine, serine, tryptophan, phenylalanine, and tyrosine. In contrast, the OW group presented the most expressive levels of glutamine, alpha-diaminopimelic, and sarcosine. Our findings indicate that obese and overweight children exhibit a particular urinary amino acid fingerprint which is similar to that reported in studies with plasma. The altered amino acids, particularly tyrosine, are frequently associated with impairments in glucose homeostasis, insulin resistance, and diabetes mellitus type 2. Potential mechanisms for increasing the levels of these amino acids in excess of weight may include enhanced protein degradation and impaired oxidative metabolism.