Kyle G.P.J.M. Boyle ¹ Andrea A. Beglinger ¹ Heinrich Häusler ¹ Anna Stahel ¹ Esther I. Schwarz ^2,3 Christina M. Spengler ^1,4*

• ¹ Exercise Physiology Lab, Institute of Human Movement Sciences and Sport, Department of Health Sciences and Technology, ETH Zurich, Zurich, Zürich, Switzerland
• ² Faculty of Medicine, University of Zurich, Zurich, Zürich, Switzerland
• ³ Department of Pulmonology, University Hospital Zurich, University of Zurich, Zurich, Switzerland, Zurich, Switzerland
• ⁴ Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland, Zurich, Switzerland

Mechanical ventilation can lead to lung injury and diaphragmatic dysfunction. Rapid bilateral anterolateral magnetic phrenic nerve stimulation (rBAMPS) may attenuate both issues by inducing diaphragm activation. However, in order for rBAMPS to become part of standard of care, the reliability of inspiratory responses to rBAMPS needs to be established. 18 healthy participants (9F) underwent five blocks of 1-s rBAMPS at 25Hz starting at 20% of maximal stimulator output with increases in 10% increments. Three blocks were completed on the same day to test within-day reliability, and two additional blocks were each completed on subsequent days to test between-day reliability. Mean transdiaphragmatic pressure (Pdi,mean), tidal volume (VT), discomfort, pain, and paresthesia were recorded for each rBAMPS. Relative and absolute reliability of both Pdi,mean and VT were quantified by calculating intraclass correlation coefficients (ICC) and standard error of measurements (SEM), respectively. An ordinal regression was used to determine changes of sensory ratings within and between days. At all stimulator outputs, within-day Pdi,mean displayed "good" reliability (ICC range 0.78 -0.89). Between days, Pdi,mean reliability was also "good" (ICC range 0.79 -0.87) at stimulator outputs of 20 to 50% of maximum, but "moderate" (ICC range 0.56 -0.72) at stimulator outputs of 60 to 100%. SEM for Pdi,mean within day ranged from 0.9 -3.4 across tested stimulator outputs, and increased on average by 1.4 ± 0.9 between days. VT reliability was "good" to "excellent" within (ICC range 0.82 -0.94) and between (ICC range 0.81 -0.96) days at all stimulator outputs. SEM for VT within day ranged from 0.08 -0.36 and from 0.11 -0.30 between days, and tended to be larger at stimulator outputs greater than 50% of maximum. Subsequent blocks within day was associated with decreased discomfort and pain (P £ 0.043), while subsequent days was associated with decreased discomfort and paresthesia (P < 0.001). rBAMPS appears to induce reliable diaphragmatic contractions, while select sensory responses become blunted over repeated stimulations. However, as reliability is slightly lower between days compared to within day, stimulation parameters may need to be adjusted to achieve similar responses on different days.