Georg Langen ^1,2* Frank Warschun ³ Olaf Ueberschär ^3,4 Michael Behringer ¹

• ¹ Department of Sports Medicine and Performance, Goethe University Frankfurt, Frankfurt, Hesse, Germany
• ² Department of Strength, Power and Technical Sports, Institute for Applied Training Science, Leipzig, Germany
• ³ Department of Biomechanics and Sport Technology, Institute for Applied Training Science, Leipzig, Germany
• ⁴ Department of Engineering and Industrial Design, Magdeburg Stendal University of Applied Sciences, Magdeburg, Saxony-Anhalt, Germany

Introduction: Tensiomyography (TMG) assesses skeletal muscle contractile properties based on the electrically stimulated radial muscle displacement. As the relationship between twitch displacement and associated torque is poorly understood, it is unclear how it is affected by post-activation potentiation and muscle fatigue. This study investigated how the interaction of potentiation and fatigue affects the rectus femoris (RF) twitch displacement and associated torque. Materials and Methods: Sixteen resistance-trained men (n = 8) and women (n = 8) performed two sets of five and five sets of ten seated maximum voluntary isometric knee extensions to induce potentiation and fatigue. Twitch displacement and torque were measured at baseline before the first set, after each set, and every two minutes for 15 minutes after the last set. Results: The exercise effectively induced potentiation and fatigue as peak twitch torque increased by 44.1 % after the first set, decreased by 32.9 % after the last set and remained decreased by 26.4 % after 15 minutes. Twitch displacement was considerably less affected by the exercise. Consequently, TMG parameters could not accurately detect potentiated or fatigued participants as indicated by the peak twitch torque. Discussion: The TMG parameters' insufficient diagnostic accuracy likely resulted from a reduced signal-to-noise ratio at 90° knee flexion and the associated longer muscle length of the RF, compared to more extended knee angles commonly employed in TMG studies. These results highlight an important methodological consideration as the joint angle, i.e. muscle length, appears to influence the TMG parameters' ability to detect exercise-induced changes in contractile properties.