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ORIGINAL RESEARCH article

Front. Physiol.
Sec. Striated Muscle Physiology
Volume 15 - 2024 | doi: 10.3389/fphys.2024.1483594

Entacapone Alleviates Muscle Atrophy by Modulating Oxidative Stress, Proteolysis, and Lipid Aggregation in Multiple Mice Models

Provisionally accepted
Rong Zeng Rong Zeng Hanbing Xu Hanbing Xu Xianlong Zhou Xianlong Zhou Jiangtao Yu Jiangtao Yu HAOLI MA HAOLI MA *Yan Zhao Yan Zhao *
  • Zhongnan Hospital, Wuhan University, Wuhan, China

The final, formatted version of the article will be published soon.

    Background: Skeletal muscle atrophy significantly affects quality of life and has socio-economic and health implications. This study evaluates the effects of entacapone (ENT) on skeletal muscle atrophy linked with oxidative stress and proteolysis. Methods: C2C12 cells were treated with dexamethasone (Dex) to simulate muscle atrophy. Four murine models were employed: diaphragm atrophy from mechanical ventilation, Dex-induced atrophy, lipopolysaccharide (LPS)-induced atrophy, and hyperlipidemia-induced atrophy. Each model utilized entacapone (10 mg/kg), with sample sizes: Control (9), MV (11), MV+ENT (5) for diaphragm atrophy; Control (4), Dex (4), Dex+ ENT (5) for Dex model; Control (4), LPS (4), LPS+ENT (5) for LPS model; and similar for hyperlipidemia. Measurements included muscle strength, myofiber cross-sectional area (CSA), proteolysis, oxidative stress markers (uperoxide dismutase 1 (SOD1), uperoxide dismutase 2 (SOD2), 4-hydroxynonenal (4-HNE)), and lipid levels. Results: Our findings confirm Dex-induced muscle atrophy, evidenced by increased expression of muscle atrophy-associated proteins, including Atrogin-1 and Murf-1,

    Keywords: entacapone, muscle atrophy, Oxidative Stress, Proteolysis, Lipid 76 aggregation 77

    Received: 22 Aug 2024; Accepted: 13 Nov 2024.

    Copyright: © 2024 Zeng, Xu, Zhou, Yu, MA and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    HAOLI MA, Zhongnan Hospital, Wuhan University, Wuhan, China
    Yan Zhao, Zhongnan Hospital, Wuhan University, Wuhan, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.