Peter J. Hanley- IMM Institute for Molecular Medicine, HMU Health and Medical University Potsdam, Potsdam, Germany
Prostatic acid phosphatase (PAP) exists as two splice variants, secreted PAP and transmembrane PAP, the latter of which is implicated in antinociceptive signaling in dorsal root ganglia. However, PAP is predominantly expressed in the prostate gland and the physiological role of seminal PAP, first identified in 1938, is largely unknown. Here, the author proposes that PAP, following ejaculation, functions to hydrolyze phosphocholine (PC) in seminal fluid and generate choline, which is imported by sperm via a choline transporter and converted to acetylcholine (ACh) by choline acetyltransferase. Auto- and paracrine cholinergic signaling, or choline directly, may subsequently stimulate sperm motility via α7 nicotinic ACh receptors (nAChRs) and contractility of the female reproductive tract through muscarinic ACh receptors (mAChRs). Consistent with a role of PAP in cholinergic signaling, 1) seminal vesicles secrete PC, 2) the prostate gland secretes PAP, 3) PAP specifically catalyzes the hydrolysis of PC into inorganic phosphate and choline, 4) seminal choline levels increase post-ejaculation, 5) pharmacological inhibition of choline acetyltransferase inhibits sperm motility, 6) inhibition or genetic deletion of α7 nAChRs impairs sperm motility, and 7) mAChRs are expressed in the uterus and oviduct (fallopian tube). Notably, PAP does not degrade glycerophosphocholine (GPC), the predominant choline source in the semen of rats and other mammals. Instead, uterine GPC phosphodiesterases may liberate choline from seminal GPC. In summary, the author deduces that PAP in humans, and uterine GPC phosphodiesterases in other mammals, function to generate choline for sperm cholinergic signaling, which promotes sperm motility and possibly contractility of the female reproductive tract.
1 Introduction
Sperm maturation, including the capacity for motility and fertilization, increases along the length of the epididymis, between caput epididymis and cauda epididymis (Bedford, 1963; Yeung et al., 1993). Following ejaculation, sperm combine with secretions from the accessory sex glands, the prostate and seminal vesicles. These secretions not only promote sperm motility (Yanagimachi, 2022), but also temporarily ensnare the sperm. Zn2+-binding semenogelin secreted by the seminal vesicles forms a gel-like matrix (Lilja and Laurell, 1984; Lilja and Laurell, 1985; Lilja et al., 1987; Anamthathmakula and Winuthayanon, 2020), seminal coagulum (copulatory plug), which traps sperm, accounting for only 2%–5% of the seminal fluid volume, and prevents premature capacitation (Juyena and Stelletta, 2012), the ability of sperm to fertilize an oocyte (Chang, 1951; Austin, 1952; Puga Molina et al., 2018; Yanagimachi, 2022). The gel-like clot formed by semenogelin not only helps to retain sperm within the female reproductive tract, but it also provides protection from the hostile acidic environment of the vagina. The prostate secretes Zn2+-inhibited prostate-specific antigen (PSA), a serine protease, which becomes activated following Zn2+ sequestration by semenogelin (Lilja et al., 1987; Malm et al., 2007; Anamthathmakula and Winuthayanon, 2020). Activated PSA cleaves semenogelin, thereby liquefying the semen within 15–20 min (Malm et al., 2007). Semenogelin, also known as seminal vesicle secretion 2, is encoded by a single gene, Semg1, in mice and by two genes, SEMG1 and SEMG2, in humans. Kawano et al. (2014) showed that homozygous Semg1 knockout male mice produce smaller litter sizes and copulatory plugs are not formed in female mice mated with male homozygous mutants. However, the percentages of motile sperm and hyperactivated sperm, a state of highly vigorous motility (acquired in the oviduct) first described by Yanagimachi (2022), are not decreased in homozygous Semg1 knockout mice. The authors also showed that uterine fluid, but not fluid from the ampulla of the oviduct, is cytotoxic to sperm, and deduced that semenogelin provides protection from the spermicidal environment of the uterine cavity. Thus, the mixing of fluids from the prostate (Zn2+-inhibited PSA) and the seminal vesicles (Zn2+-binding semenogelin) enable the formation of a gel and its subsequent liquefaction, which releases sperm with a protective coat. In addition to semenogelin, a thick glyocalyx, acquired during maturation in the epididymis, help sperm to cross the uterus and survive uterine immunity (Tecle and Gagneux, 2015; Lan et al., 2020).
In addition to PSA, the prostate secretes prostatic acid phosphatase (PAP), encoded by ACPP in humans and Acp3 (acid phosphatase 3) in mouse. The physiological function of this enzyme in seminal plasma is unclear. In this mini-review, the author distills and analyzes the literature, providing an evidence-based framework for understanding the physiological role of seminal PAP, while also shedding new light on the functions of seminal choline and cholinergic signaling in sperm.
2 Antinociceptive function of PAP in dorsal root ganglia
PAP exists in two widely expressed isoforms in both human and mouse, generated by alternative splicing that either includes or excludes a C-terminal transmembrane domain: transmembrane PAP, a type-I transmembrane protein, and secreted PAP (Quintero et al., 2007). Both isoforms exhibit 5′-ectonucleotidase activity and accordingly degrade (extracellular) adenosine monophosphate to adenosine (Zylka et al., 2008; Street et al., 2013; Araujo et al., 2014). Transmembrane PAP is the predominant isoform expressed in dorsal root ganglia, where it has been deduced to generate adenosine and exert an antinociceptive effect through stimulation of adenosine A1-receptors (Zylka et al., 2008). Nevertheless, in human, PAP is predominantly expressed in the prostate and from hereon the abbreviation PAP refers to the secreted form.
3 PAP liberates choline from seminal gland-secreted phosphocholine
Lundquist reported in 1946 (Lundquist, 1946) that the levels of inorganic phosphate are low immediately following ejaculation, but rapidly increase within minutes. The author deduced that inorganic phosphate and choline are produced when an acid phosphatase secreted by the prostate, previously described by Kutscher and Wolbergs (1935), hydrolyzes phosphocholine (PC), secreted by the seminal glands. Thus, the mixing of PC from seminal vesicles and PAP from the prostate produces choline in the ejaculate, as schematically shown in Figure 1. Consistent with this model, PC has been shown to be a highly specific substrate for PAP (Seligman et al., 1975; Serrano et al., 1977), a tartrate-sensitive member of the histidine phosphatase superfamily (McTigue and Van Etten, 1978; Jakob et al., 2000; Rigden, 2008; Ara et al., 2013). Indeed, PC is hydrolyzed by human PAP (Seligman et al., 1975; Serrano et al., 1977), but not human liver acid phospatase (Saini and Van Etten, 1978), and this substrate specificity has been exploited in the past to quantify serum phosphatase activity originating from the prostate (Saini and Van Etten, 1981). PAP activity per milligram of prostate varies widely among species and is notably 1,000 times or more higher in humans than rodents (Seligman et al., 1975).
FIGURE 1. Schematic diagram depicting seminal choline production in humans through prostatic acid phosphatase (PAP) and in other mammals via uterine diesterases. (A) in humans, PAP serves to hydrolzye phosphocholine (PC) and produce choline. (B) in mice and other mammals, choline is primarily produced through uterine diesterases acting on glycerophosphocholine (GPC), which is primarily secreted by the epididymis.
In further work, Lundquist (1953) observed that the mixing of saline extract from the prostate of the rat with extracts from the seminal vesicles produced much more free choline than could be accounted for by hydrolysis of available PC. This suggested the presence of an alternative source of choline, which was identified as glycerophosphocholine (GPC). High levels of GPC, relative to PC and normalized to unit moist tissue weight, were found in rat, guinea-pig, and rabbit in the order: rat > guinea-pig > rabbit. Dawson et al. (1957) confirmed that PC is the predominant source of choline in human seminal plasma. Human semen also contains some GPC, but unlike PC, it does not degrade during semen incubation (Dawson et al., 1957). In contrast to humans, high levels of GPC, but only trace levels or no PC, were detected in the semen of ram, bull, goat, boar, rat, and rabbit. The source of GPC has been localized to the epididymis (Scott et al., 1963; Wallace et al., 1966; Arrata et al., 1978; Hinton and Setchell, 1980; Hoffmann and Killian, 1981) (Figure 1). Assuming that choline plays a role in fertility, the lack of infertility or subinfertility observed in mice lacking Acp3 (Quintero et al., 2013) could be explained by the availability of an alternative substrate, GPC, for generation of choline. However, Acp3 knockout mice develop prostate adenocarcinoma (Quintero et al., 2013) and exhibit susceptibility to chronic pain (Zylka et al., 2008). White and Wallace (1961) showed that secretions from the female reproductive tract could hydrolyze GPC. Preheating of uterine secretions to 100 °C for 5 min prevented GPC hydrolysis, suggesting that an enzyme, presumably a diesterase, is responsible. A Ca2+-dependent diesterase, with exclusive specificity for GPC, has been purified from rat uterine secretions (Mitra and Chowdhury, 1994). This enzyme catalyzes the hydrolysis of GPC to choline and glycerol 3-phosphate. A likely candidate for this enzyme is ectonucleotide pyrophosphatase/phosphodiesterase (ENPP) family member 6 (ENPP6), which has substrate specificity for GPC (Borza et al., 2022). ENPP6 exists in two forms: a glycosylphosphatidylinositol (GDI)-anchored enzyme and, after cleavage of the GDI-anchor, a soluble enzyme. However, ENPP6 is predominantly expressed in the brain and kidney (Greiner-Tollersrud, 2014), although it has been detected in bovine uterine fluids (Piibor et al., 2023). Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) is widely expressed in humans, including in the uterus, and can also generate choline from GPC (Gallazzini et al., 2008; Lang et al., 2008). Deletion of Gdpd5, previously denoted Gde2, in mice and interbreeding produced homozygotes in the expected Mendelian ratios, which exhibited normal fertility (Sabharwal et al., 2011), although abnormal cortical neuron development was observed (Sabharwal et al., 2011; Rodriguez et al., 2012). Alternatively, choline can also be generated in the uterus by the secreted enzyme ENPP2 (Ahn et al., 2011), which converts lysophosphatidylcholine (LPC) to lysophosphatidic acid (LPA) (Goding et al., 2003; Seo et al., 2012). Mice lacking ENPP2 (encoded by Enpp2), also known as autotaxin or lysophospholipase D, die at midgestation and exhibit abnormal vascular development (van Meeteren et al., 2006), whereas homozygous Enpp6 knockout mice are viable and fertile (Morita et al., 2016). Interestingly, human semen has been shown to exhibit ENPP2 activity and PAP was deduced to degrade the product LPA (Tanaka et al., 2004), thereby driving the ENPP2-catalyzed reaction through mass action to produce more choline. Thus, whereas choline can be produced from PC by PAP in humans, choline can be derived from GPC, or possibly LPC, in other mammals via various uterine phosphodiesterases.
Choline production within the female reproductive tract after sexual intercourse seems to be a shared phenomenon across various species, suggesting that this quaternary ammonium cation plays a specific role in sperm function and fertility. On one hand, choline serves as a precursor for the synthesis of specific phospholipids, including phosphatidylcholine and sphingomyelin. On the other hand, choline is essential for the production of the neurotransmitter acetylcholine (ACh). While the notion of sperm synthesizing ACh may appear counterintuitive, it should be noted that non-neuronal cholinergic (ACh-mediated) signaling has been extensively implicated in the reproductive system (Wessler and Kirkpatrick, 2017).
4 Hypothesis: choline derived from PC, GPC, or LPC drives sperm motility, as well as uterine contractility, via auto- and paracrine cholinergic signaling
The author speculates that choline is taken up by sperm via a choline transporter and converted via choline acetyltransferase (ChAT) to ACh, which is subsequently released via a presumably non-vesicular pathway to stimulate sperm motility and/or uterine contractile activity via auto- and paracrine activity, respectively, as schematically illustrated in Figure 2A. Pharmacological inhibition of ChAT, which catalyzes the synthesis of ACh by transferring an acetyl group from acetyl-CoA to choline, has been shown to inhibit the motility of human sperm (Sastry et al., 1981). Consistent with these observations, sperm have been shown to express Chat mRNA and immunofluorescence imaging revealed intense localization of ChAT to the post-acrosomal region in the head of mature sperm (Ibáñez et al., 1991). Genetic deletion of Chat in a mouse model could help to elucidate putative roles of cholinergic signaling in sperm motility and/or fertility. However, ChAT is critical for neuronal cholinergic signaling and deletion of Chat in mice is lethal (Misgeld et al., 2002). Homozygous Chat knockout mice exhibit flaccid paralysis and die shortly after birth. This lethal phenotype could be explained by loss of presynaptic ACh synthesis since ChAT activity could not be detected in brain lysates from homozygous mutants and stimulation of motor neurons failed to evoke excitatory postsynaptic potentials in myotubes from homozygous mutant embryos. In principle, lethality could be circumvented by crossing floxed Chat mice, such as B6; 129-Chattm1Jrs/J mice, in which loxP (Cre recombinase-recognition) sites flank exons 3 and 4 of the Chat gene, with a sperm-restricted Cre recombinase mice, such as Stra8-Cre mice (Sadate-Ngatchou et al., 2008), in which Cre recombinase expression is driven by the gene Stra8 (stimulated by retinoic acid gene 8), a gene specifically activated in spermatocytes as they enter meiosis.
FIGURE 2. Schematic diagram of sperm motility induced by the generation of choline via prostatic acid phosphatase (PAP) and driven by cholinergic signaling. (A) choline liberated from either phosphocholine (PC) in humans or glycerophosphocholine (GPC) in other mammals is imported into sperm via a choline transporter and converted to acetylcholine (ACh) through choline acetyltransferase (ChAT). Sperm are unable to store ACh, which is released to the extracellular space via an organic cation transporter novel (OCTN) or other transporter, where it stimulates Ca2+-permeable α7 nicotinic ACh receptors (nAChRs). Increased intracellular [Ca2+] ([Ca2+]i) promotes flagellar beating and sperm motility. Choline can directly activate α7 nAChRs, albeit with less potency than ACh. Sperm also express a Ca2+-selective channel, known as the cation channel of sperm (CatSper), which is not required for initial post-ejaculation activated motility, but is essential for hyperactivated motility and fertility. (B) cholinergic signaling induced by the availability of free choline (shown above) probably serves to promote so-called activated motility, characterized by low-amplitude and symmetrical beating of the flagellum, and migration of sperm from the vagina and/or uterus to uterotubal junctions. In the oviduct, capacitation leads to the activation of CatSper, which is required for hyperactivated motility, characterized by high-amplitude and asymmetrical beating of the flagellum, and fertility, in particular, penetration of the cumulus oophorus and zona pellucida.
In addition to Chat, sperm have been shown to express the high-affinity choline transporter SLC5A7, encoded by Slc5a7 in rodents (Schirmer et al., 2011). Deletion of Slc5a7 in mice, similar to deletion of Chat, is lethal. Homozygous Slc5a7 knockout mice appear morphologically normal immediately after birth, but exhibit immobility and severely impaired breathing, which leads to cynanosis and death within 1 hour (Ferguson et al., 2004), which can be explained by lack of availability of the neurotransmitter ACh presynaptically at neuromuscular junctions. As in the case of Chat, conditional deletion of Slc5a7 in the sperm of mice could help to confirm whether the choline transporter SLC5A7 is important for sperm motility and/or fertility. Mice harboring the knockout first allele (tm1a), Slc5a7tm1a(KOMP)Wtsi mice, which can be converted to a floxed allele (tm1c), have been produced by the International Mouse Phenotyping Consortium (IMPC) and could be used to generate sperm-restricted Slc5a7 knockout mice, for example, by crossing floxed Slc5a7 mice with Stra8-Cre mice.
Acetylcholine synthesized via ChAT within sperm, which lack the capacity for its storage (Bishop et al., 1976), is presumably released through a cation transporter, as shown in Figure 2A. Human sperm express members of the organic cation transporter novel (OCTN) subfamily of the solute carrier (SLC) superfamily, including OCTN1 and OCTN2 (Xuan et al., 2003). OCTN1, encoded by Slc22a4 in mouse, has been shown to transport ACh, which is competitively inhibited by the synthetic transporter substrate tetraethylammonium (Pochini et al., 2012; Pochini et al., 2019). Mice lacking Slc22a4 are fertile, but sperm motility was not investigated (Kato et al., 2010). However, ACh, as well as choline and carnitine, may be transported to various degrees by other OCTNs expressed in mouse sperm (Kobayashi et al., 2007). Notably, both human and rat OCTN1 and OCTN2, heterologously expressed in oocytes from Xenopus laevis, have been shown to release ACh (Lips et al., 2005). Released ACh could stimulate ACh receptors expressed on sperm or in the female reproductive tract. Indeed, ACh has been shown to increase sperm motility in various species, including sea urchin, bull, chimpanzee, and human (Nelson, 1972; McGrady and Nelson, 1976; Dwivedi and Long, 1989), whereas findings for mouse are inconsistent (Sliwa, 1995; Makino et al., 2021).
ACh receptors are classified into two main types: nicotinic ACh receptors (nAChRs), which are ACh-gated ion channels mainly found in the nervous system and skeletal muscle, and muscarinic ACh receptors (mAChRs), G protein-coupled receptors predominantly located at targets of the parasympathetic nervous system, including the heart, smooth muscle, and glands. nAChRs are heteropentamers permeable to Na+ and K+ and consist of two identical α-subunits and three distinct subunits, which can be β, γ, δ, or ε (Skok, 2022). Homopentameric α7 nAChRs have also been identified, which are additionally permeable to Ca2+ (Delbono et al., 1997). Interestingly, choline has been shown to be a selective full agonist of α7 nAChRs (Papke et al., 1996; Alkondon et al., 1997; Mike et al., 2000), albeit with one order of magnitude lower potency than ACh (Alkondon et al., 1997). Mice lacking Chrna7, which encodes the α7 subunit, are viable and exhibit no obvious developmental or neurological disorders (Orr-Urtreger et al., 1997). Homozygous male and female Chrna7 knockout mice were reported to be fertile, but the authors raised nonspecified potential fertility issues. Indeed, Bray et al. (2005) found that sperm from Chrna7 knockout mice had significantly impaired motility and a lower rate of hyperactivation, whereas the number, morphology, and viability of sperm were normal. These findings align with the observation that various nAChR inhibitors, including the snake toxin α-bungarotoxin, as well as other nAChR antagonists like hexamethonium and succinylcholine, inhibit sperm motility (Nelson, 1976; Dwivedi and Long, 1989). In addition to the α7 subunit, the inhibitor α-bungarotoxin binds to the α9 subunit, which is also expressed in sperm (Kumar and Meizel, 2005). Immunofluorescence imaging revealed distinct localization patterns of α7 and α9 subunits in sperm. Specifically, α7 subunits are prominently localized to the post-acrosomal regions encompassing the head, neck (connecting piece), midpiece, and the principal and terminal pieces of the tail (flagellum) (Bray et al., 2005; Kumar and Meizel, 2005) In contrast, α9 subunits exhibit a more restricted localization, being detectable in approximately 50% of sperm and primarily localizing to the acrosomal region (Makino et al., 2021). Makino et al. (2021) did not observe increased motility with ACh treatment, but instead the acrosome reaction rate was decreased, suggesting that nAChRs containing α9 subunits regulate the acrosome reaction.
Extracellular Ca2+ is essential for sperm motility, highlighted by the observation that removal of Ca2+ halts motility, while reintroduction reactivates it (Feng et al., 1988; Aaberg et al., 1989; Ignotz and Suarez, 2005; Jin et al., 2007; Torres-Flores et al., 2011). Auto- and paracrine cholinergic signaling, and possibly direct choline-mediated signaling, may increase intracellular [Ca2+] and promote the low-amplitude and symmetrical mode of flagellar beating associated with activated motility (Turner, 2006), required for freshly ejaculated sperm to move with high directionality through the female reproductive tract (Turner, 2006; Suarez, 2016). In addition, non-neuronal cholinergic signaling driven by choline availability may also stimulate peristaltic smooth muscle contractions of the female reproductive tract, which help to propel sperm, or sperm aggregates, towards and beyond the uterotubal junctions (Wang and Larina, 2023). That is, following ejaculation, choline may directly stimulate Ca2+ influx into the tail via activation of α7 nAChRs or indirectly via the pathway of choline uptake, ACh synthesis, and ACh release (Figure 2A). Cholinergic receptor stimulation is probably cyclical due to a combination of ACh hydrolysis by acetylcholinesterases (AChEs), expressed in sperm (Nelson, 1964; Chakraborty and Nelson, 1976; Stewart and Forrester, 1978; Egbunike, 1980; Egbunike, 1982), and α7 nAChR desensitization (Papke et al., 2009). At the isthmus of the oviduct, sperm accumulate (Suarez, 2008) and capacitation procedes, involving removal of plasma membrane cholesterol and intracellular alkalinization, which leads to activation of a Ca2+-selective channel, known as the cation channel of sperm (CatSper) (Ren et al., 2001; Kirichok et al., 2006). This channel is localized to the principal piece of sperm (Chung et al., 2017; Wang et al., 2021) and is essential for hyperactivated motility (Turner, 2006), a mode of vigorous high-amplitude and asymmetrical beating of the tail required for sperm to ascend the oviduct, albeit with low directionality, and penetrate the cumulus oophorus, a mass of granulosa cells enveloping the oocyte, and zona pellucida (Ho et al., 2009; Chang and Suarez, 2012). Deletion of any one of the four genes (Catsper1, Catsper2, Catsper3, or Catsper4) encoding its pore-forming subunits (Lin et al., 2021) causes infertility (Jin et al., 2007; Ren et al., 2001; Quill et al., 2003; Qi et al., 2007). Thus, auto- and paracrine cholinergic stimulation of sperm α7 nAChRs, possibly together with stimulation of uterine smooth muscle mAChRs (Agha et al., 2001), may promote sperm migration and transport to the uterotubal junction and oviduct, where capacitation and activation of CatSper stimulate hyperactivated motility, required for further intraoviductal ascend and penetration of the oocyte, as delineated in Figure 2B.
Aside from the generation of choline to support cholinergic signaling, the 5′-ectonucleotidase activity of PAP, which generates adenosine from AMP, may contribute to purinergic signaling in sperm and the female reproductive tract. Adenosine has been shown both to stimulate sperm capacitation (Fraser and Duncan, 1993; Fénichel et al., 1996) and to increase the motility of bovine and human sperm in a dose-dependent fashion (Vijayaraghavan and Hoskins, 1986; Shen et al., 1993). Adenosine receptors (ARs), expressed in sperm (Bellezza and Minelli, 2017), can be categorized into two types of G protein-coupled receptors: A1R and A3R, which are Gi-coupled, and A2aR and A2bR, which are Gs-coupled. Mice lacking A1Rs, encoded by Adora1, exhibit normal sperm motility, but take much longer to acquire capacitation (Minelli et al., 2004). This possibly accounts for the ∼40% reduction in average litter size (Minelli et al., 2004). Mice lacking A3Rs, encoded by Adora3, are fertile and exhibit no obvious developmental abnormalities (Salvatore et al., 2000). Similarly, mice lacking the A2aR, encoded by Adora2a, are viable and fertile, but exhibit reduced exploratory behavior, among other phenotypes (L et al., 1997; Yaar et al., 2005). Mice engineered to lack A2bR, encoded by Adora2b, and contain a gene expression reporter (lacZ; which encodes β-galactosidase) were found to breed normally (Yang et al., 2006). However, the authors showed that Adora2b is expressed in the vasculature and macrophages and Adora2b-deficient mice exhibit low-grade inflammation, consistent with an inhibitory action of Gs-coupled receptor stimulation on proinflammatory signal pathways. Independently, the IMPC have generated Adora1, Adora2a, and Adora2b knockout mouse models and infertility was not observed during phenotype screening. Thus, PAP may potentiate sperm motility by generating adenosine from AMP, although adenosine receptor knockout mouse models have not implicated these receptors in sperm motility or fertility. Further research is needed to clarify the physiological significance of adenosine-induced sperm motility.
5 Conclusion
The author proposes that the physiological function of human PAP is to produce choline from PC, which promotes sperm motility via cholinergic signaling. In rodents and other mammals, GPC, rather than PC, is the primary source of choline, which is harvested by uterine diesterases. Across species, the author proposes that choline, in addition to directly stimulating α7 nAChRs, stimulates directional sperm motility via repeated cycles of uptake of choline by the high-affinity choline transporter, generation of ACh from choline via ChAT, release of ACh via an OCTN or similar transporter, auto- and paracrine stimulation of α7 nAChRs, influx of Ca2+, and ACh hydrolysis via AChEs. PAP may also generate adenosine from AMP, which may further promote sperm motility via adenosine receptors. These hypotheses are supported by the availability of all the essential molecular components in sperm and seminal plasma, as well as previously published experimental data substantiating the various steps.
Author contributions
PH: Writing–original draft.
Funding
The author(s) declare that no financial support was received for the research, authorship, and/or publication of this article.
Conflict of interest
The author declares that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Keywords: prostatic acid phosphatase (PAP), phosphocholine (PC), choline, cholinergic signaling, sperm motility, acetylcholine (ACh), acetylcholine receptor (AChR)
Citation: Hanley PJ (2023) Elusive physiological role of prostatic acid phosphatase (PAP): generation of choline for sperm motility via auto-and paracrine cholinergic signaling. Front. Physiol. 14:1327769. doi: 10.3389/fphys.2023.1327769
Received: 25 October 2023; Accepted: 07 December 2023;
Published: 22 December 2023.
Edited by:
Trish Berger, University of California, Davis, United StatesReviewed by:
Volodymyr Tsvilovskyy, Heidelberg University, GermanyCopyright © 2023 Hanley. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Peter J. Hanley, cGV0ZXIuaGFubGV5QGhlYWx0aC1hbmQtbWVkaWNhbC11bml2ZXJzaXR5LmRl