AUTHOR=Fan Wenjuan , Tan Qinghai 

TITLE=Application of the steady-state intestinal perfusion system in measuring intestinal fluid absorption and bicarbonate secretion in vivo

JOURNAL=Frontiers in Physiology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2023.1163888

DOI=10.3389/fphys.2023.1163888

ISSN=1664-042X

ABSTRACT=<p><bold>Background:</bold> The steady-state intestinal perfusion system represents a tool used in measuring intestinal fluid absorption and bicarbonate secretion <italic>in vivo</italic>; however, detailed procedures and parameters were not elucidated fully.</p><p><bold>Aim:</bold> We focused on the methods of the steady-state intestinal perfusion system comprehensively including the blood pressure, hematocrit, blood gas, and heart rate of mouse.</p><p><bold>Methods:</bold> Anesthetized, tracheally intubated, and artificially ventilated mice were used for this system. The blood pressure, hematocrit, blood gas, heart rate, and rate of fluid absorption and HCO<sub>3</sub><sup>-</sup> secretion of the small intestine and colon at different time points were evaluated.</p><p><bold>Results:</bold> Blood pressure, hematocrit, blood gas, and heart rate became stable at the 30 min time point after completion of surgery and could be maintained for 2 h. Rates of fluid absorption and bicarbonate secretion were also kept stable during the period of steady state of mice. Rates of fluid absorption and bicarbonate secretion were different among the jejunum, ileum, proximal, and mid-distal colon.</p><p><bold>Conclusion:</bold> The steady-state intestinal perfusion system is a reliable system for measuring intestinal fluid absorption and bicarbonate secretion <italic>in vivo.</italic></p>