- 1Department of Kinesiology, Storrs, CT, United States
- 2UConn Center on Aging and Department of Orthopedic Surgery, University of Connecticut, Storrs, CT, United States
- 3Graduate School of Health and Sport Science, Nippon Sport Science University, Tokyo, Japan
- 4Department of Physical Therapy and Kinesiology, University of Massachusetts Lowell, Lowell, MA, United States
Editorial on the Research Topic
New insights into the role of mitochondria in muscle pathophysiology
Introduction
Over the last decades there has been a big progress in defining roles of mitochondria in the cell. However, there are still unsolved questions as to how these organelles alter their molecular and functional characteristics under physiological and pathological challenges, particularly in skeletal muscle where mitochondria are key to metabolism, movement, and body temperature. Here, this Research Topic was designed to provide new insights into the roles of mitochondria in muscle physiology and had a great opportunity to collect prominent research articles that, using animal and cell models, demonstrate underlying molecular events important for mitochondrial oxidative metabolism in skeletal muscle at various physiological and pathological conditions (i.e., diabetes (Bhardwaj et al.), high-intensity interval training (HIIT) (Delfan et al.), lactate overload (Takahashi et al.), and muscle degeneration (Palzkill et al.).
Key findings
1. Insulin plays a crucial role for mitochondrial function through interacting with insulin receptor (IR) and IGF-1 receptor (Taniguchi et al., 2006), yet the underlying mechanism is obscure especially in skeletal muscle. Using double knockout mouse model (e.g., co-deletion of IR and IGF-1 receptor), Bhardwaj et al. showed that the absence of both IR and IGF-1 receptor leads to mitochondrial dysfunction in skeletal muscle and it is related with significant changes in muscle transcriptome. Notably, they revealed that these transcriptomic regulations are dependent on the transcription factor Forkhead box O proteins (FoxOs), as mitochondria-specific molecular changes (e.g., TCA cycle genes) are normalized in the absence of FoxOs. Furthermore, this study also pointed out a critical role of FoxOs in the regulation of genes for muscle calcium signaling pathways (e.g., sarcoplasmic reticulum, transcriptional regulation, and muscle contraction). Collectively, it is likely that FoxOs are central players for the insulin action for mitochondrial capacity, as well as calcium handling in skeletal muscle.
2. Using high-intensity interval training (HIIT) model, Delfan et al. showed that this time-efficient exercise training protocol is capable of increasing mitochondrial biogenesis markers (e.g., PGC-1α, Citrate Synthase, and p53) in the skeletal muscle of diabetic animal model (i.e., streptozotocin-injected Wistar rats). In this study, they suggested that HIIT training with a higher work-to-rest ratio (HIIT with long recovery exercise; 2 min of high intensity running followed by low-intensity recovery exercise for 2 min) results in more significant increase in mitochondrial aerobic capacity in the diabetic soleus muscles as compared to the ones with a lower work-to-rest ratio (HIIT with short recovery exercise; 2 min of high intensity running followed by low-intensity recovery exercise for 1 min). Therefore, when applying HIIT, the interval length may be considered in order to improve muscle aerobic capacity.
3. Takahashi et al. suggested that lactate is a potent stimulator for upregulating mitochondrial respiratory capacity in skeletal muscle. Following long-term administration (4 weeks) of lactate via intraperitoneal injections, they observed a significant increase in mitochondrial oxidative capacity (e.g., state three oxygen consumption rate) in mouse skeletal muscle (i.e., gastrocnemius). In particular, it appears that these lactate-induced mitochondrial adaptations in skeletal muscle can be achieved by enhanced enzyme activity and abundance for mitochondrial electron transport chain complex I. Thus, the outcomes of this study indicate that high intensity exercise training can improve mitochondrial oxidative metabolism in skeletal muscle through activating lactate production.
4. Given that L-Kynurenine (L-Kyn), byproduct of tryptophan metabolism, is related with muscle weakness, atrophy, and neuromuscular dysfunction, Palzkill et al. sought to understand how the elevated L-Kyn affects mitochondrial function in skeletal muscle. Following 10 weeks of dietary treatment with L-Kyn (150 mg/kg), they observed that, although muscle size and contractile function are not changed, mitochondrial respiratory capacity (e.g., mitochondrial oxygen consumption) is significantly diminished in mouse skeletal muscle. Based on upregulated mRNA level of PGC-1α, this study suggests that upon elevated L-Kyn, PGC-1α may exert a compensatory response in that skeletal muscle. Using in vitro model, they further demonstrated that lentiviral-mediated PGC-1α overexpression has a significant effect on preserving mitochondrial function, as well as myotube structure, suggesting a possible protective role of PGC-1α against L-kyn-related muscle mitochondrial dysfunction.
Closing comments
In this Research Topic, we can pull out several key markers for mitochondrial function, which is valuable to find possible therapeutic targets for maintaining or improving muscle health. Furthermore, these findings can be further developed to understand how those molecular systems are linked with other mitochondrial control systems such as mitochondrial turnover (fusion/fission), quality control (mitophagy), and structural changes in skeletal muscle.
Author contributions
All authors listed have made a substantial, direct, and intellectual contribution to the work and approved it for publication.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Reference
Keywords: mitochondria, skeletal muscle, FoxO, HIIT, lactate, L-kynurenine
Citation: Kwon OS, Tamura Y and Kim Y (2023) Editorial: New insights into the role of mitochondria in muscle pathophysiology. Front. Physiol. 14:1151120. doi: 10.3389/fphys.2023.1151120
Received: 25 January 2023; Accepted: 02 February 2023;
Published: 07 February 2023.
Edited and reviewed by:
Jason Bazil, Michigan State University, United StatesCopyright © 2023 Kwon, Tamura and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Yuho Kim, eXVob19raW1AdW1sLmVkdQ==