AUTHOR=Zhang Ruijuan , Ji Jinliang , Li Yabin , Yu Jianbin , Yu Xiaoping , Xu Yipeng 

TITLE=Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål)

JOURNAL=Frontiers in Physiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.853956

DOI=10.3389/fphys.2022.853956

ISSN=1664-042X

ABSTRACT=<p><italic>SLC26A10</italic> is a member of the SLC26 gene family, but its role in insects is still unclear. We cloned the <italic>SLC26A10</italic> gene of <italic>Nilaparvata lugens</italic> (<italic>NlSLC26A10</italic>) and found <italic>NlSLC26A10</italic> contained 11 transmembrane regions and a STAS domain. Expression pattern analysis showed <italic>NlSLC26A10</italic> expression was more upregulated in adults than in nymphs, highest in the ovary. After injection of double-stranded RNA (dsRNA) of <italic>NlSLC26A10</italic>, the mRNA level of <italic>NlSLC26A10</italic> significantly decreased and, consequently, the ovarian development of adult females was hindered; the amount and the hatchability of eggs and yeast-like symbionts in mature oocytes decreased. Further study showed that <italic>NlSLC26A10</italic> might result in decreased juvenile hormone level and vitellogenin expression. These results indicate that <italic>NlSLC26A10</italic> plays an essential role in the reproduction of <italic>N. lugens</italic>.</p>