AUTHOR=Xiong Yangyang , Ji Li , Zhao Yi , Liu Ailing , Wu Dong , Qian Jiaming 

TITLE=Sodium Butyrate Attenuates Taurocholate-Induced Acute Pancreatitis by Maintaining Colonic Barrier and Regulating Gut Microorganisms in Mice

JOURNAL=Frontiers in Physiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.813735

DOI=10.3389/fphys.2022.813735

ISSN=1664-042X

ABSTRACT=<sec><title>Background</title><p>Acute pancreatitis (AP) damages the intestinal barrier, which aggravates AP. Butyrate exhibits anti-inflammatory effects in AP, but it is unknown if such a protective effect is associated with the regulation of gut microorganisms. We aim to investigate the effects of sodium butyrate (SB) on pancreatic inflammation, colonic barrier, and gut microorganisms.</p></sec><sec><title>Methods</title><p>C57BL/6 mice were divided into groups of sham operation (Sham), AP, 200 mg/kg SB intervention (SB-200), and 500 mg/kg SB intervention group (SB-500). Samples were harvested 24 h after the model was established. The gut microbiota was analyzed using 16S rRNA gene sequencing.</p></sec><sec><title>Results</title><p>Pancreatic infiltration of neutrophils, macrophages, and M2-type macrophages was significantly reduced in the SB-500 intervention group. Supplementation of SB-500 improved colon mucosal histology and the expression of ZO-1 and occluding. The relative abundance of <italic>Alloprevotella</italic> and <italic>Muribaculaceae</italic> was increased and that of <italic>Akkermansia</italic> was decreased in the SB-500 group compared with the AP group. <italic>Ruminococcaceae</italic> was the most significantly increased species and <italic>Prevotellaceae</italic> was the most significantly decreased species in the SB-500 group compared with the AP group.</p></sec><sec><title>Conclusion</title><p>High dose of SB inhibits pancreatic inflammation probably by maintaining the intestinal barrier and regulating gut microbiota in mice with AP.</p></sec>