AUTHOR=Uzelac Ilija , Crowley Christopher J. , Iravanian Shahriar , Kim Tae Yun , Cho Hee Cheol , Fenton Flavio H. 

TITLE=Methodology for Cross-Talk Elimination in Simultaneous Voltage and Calcium Optical Mapping Measurements With Semasbestic Wavelengths

JOURNAL=Frontiers in Physiology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.812968

DOI=10.3389/fphys.2022.812968

ISSN=1664-042X

ABSTRACT=<p>Most cardiac arrhythmias at the whole heart level result from alteration of cell membrane ionic channels and intracellular calcium concentration ([Ca<sup>2+</sup>]<sub><italic>i</italic></sub>) cycling with emerging spatiotemporal behavior through tissue-level coupling. For example, dynamically induced spatial dispersion of action potential duration, QT prolongation, and alternans are clinical markers for arrhythmia susceptibility in regular and heart-failure patients that originate due to changes of the transmembrane voltage (<italic>V</italic><sub>m</sub>) and [Ca<sup>2+</sup>]<sub><italic>i</italic></sub>. We present an optical-mapping methodology that permits simultaneous measurements of the <italic>V</italic><sub>m</sub> - [Ca<sup>2+</sup>]<sub><italic>i</italic></sub> signals using a single-camera without cross-talk, allowing quantitative characterization of favorable/adverse cell and tissue dynamical effects occurring from remodeling and/or drugs in heart failure. We demonstrate theoretically and experimentally in six different species the existence of a family of excitation wavelengths, we termed semasbestic, that give no change in signal for one dye, and thus can be used to record signals from another dye, guaranteeing zero cross-talk.</p>