AUTHOR=Marshall Ryan Neil , McKendry James , Smeuninx Benoit , Seabright Alex Peter , Morgan Paul T. , Greig Carolyn , Breen Leigh 

TITLE=Acute resistance exercise training does not augment mitochondrial remodelling in master athletes or untrained older adults

JOURNAL=Frontiers in Physiology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.1097988

DOI=10.3389/fphys.2022.1097988

ISSN=1664-042X

ABSTRACT=<p><bold>Background:</bold> Ageing is associated with alterations to skeletal muscle oxidative metabolism that may be influenced by physical activity status, although the mechanisms underlying these changes have not been unraveled. Similarly, the effect of resistance exercise training (RET) on skeletal muscle mitochondrial regulation is unclear.</p><p><bold>Methods:</bold> Seven endurance-trained masters athletes ([MA], 74 ± 3 years) and seven untrained older adults ([OC]. 69 ± 6 years) completed a single session of knee extension RET (6 x 12 repetitions, 75% 1-RM, 120-s intra-set recovery). Vastus lateralis muscle biopsies were collected pre-RET, 1 h post-RET, and 48h post-RET. Skeletal muscle biopsies were analyzed for citrate synthase (CS) enzyme activity, mitochondrial content, and markers of mitochondrial quality control <italic>via</italic> immunoblotting.</p><p><bold>Results:</bold> Pre-RET CS activity and protein content were ∼45% (<italic>p</italic> &lt; .001) and ∼74% greater in MA compared with OC (<italic>p</italic> = .006). There was a significant reduction (∼18%) in CS activity 48 h post-RET (<italic>p</italic> &lt; .05) in OC, but not MA. Pre-RET abundance of individual and combined mitochondrial electron transport chain (ETC) complexes I-V were significantly greater in MA compared with OC, as were markers of mitochondrial fission and fusion dynamics (p-DRP-1<sup>Ser616</sup>, p-MFF<sup>Ser146</sup>, OPA-1 &amp; FIS-1, <italic>p</italic> &lt; .05 for all). Moreover, MA displayed greater expression of p-AMPK<sup>Thr172</sup>, PGC1α, TFAM, and SIRT-3 (<italic>p</italic> &lt; .05 for all). Notably, RET did not alter the expression of any marker of mitochondrial content, biogenesis, or quality control in both OC and MA.</p><p><bold>Conclusion:</bold> The present data suggest that long-term aerobic exercise training supports superior skeletal muscle mitochondrial density and protein content into later life, which may be regulated by greater mitochondrial quality control mechanisms and supported <italic>via</italic> superior fission-fusion dynamics. However, a single session of RET is unable to induce mitochondrial remodelling in the acute (1h post-RET) and delayed (48 h post-RET) recovery period in OC and MA.</p>