AUTHOR=Jiang Yuhao , Li Hongzhong , Pang Yu , Ling Jiwei , Wang Hao , Yang Yuling , Li Xinyu , Tian Yin , Wang Xiuxin TITLE=Cell image reconstruction using digital holography with an improved GS algorithm JOURNAL=Frontiers in Physiology VOLUME=13 YEAR=2022 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2022.1040777 DOI=10.3389/fphys.2022.1040777 ISSN=1664-042X ABSTRACT=

Digital holography is an effective technology in image reconstruction as amplitude and phase information of cells can be acquired without any staining. In this paper, we propose a holographic technique with an improved Gerchberg-Saxton (GS) algorithm to reconstruct cell imaging based on phase reconstruction information. Comparative experiments are conducted on four specific models to investigate the effectiveness of the proposed method. The morphological parameters (such as shape, volume, and sphericity) of abnormal erythrocytes can be obtained by reconstructing cell hologram of urinary sediment. Notably, abnormal red blood cells can also be detected in mussy circumstances by the proposed method, owing to the significantly biophysical contrast (refractive index distribution and mass density) between two different cells. Therefore, this proposed method has a broad application prospect in cell image reconstruction and cell dynamic detection.