AUTHOR=Li Dandan , Wang Pingping , Wei Wenyan , Wang Cheng , Zhong Yong , Lv Lei , Wang Junjun TITLE=Serum MicroRNA Expression Patterns in Subjects After the 5-km Exercise Are Strongly Associated With Cardiovascular Adaptation JOURNAL=Frontiers in Physiology VOLUME=Volume 12 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.755656 DOI=10.3389/fphys.2021.755656 ISSN=1664-042X ABSTRACT=Emerging evidence indicate that circulating microRNAs (miRNAs) are dysregulated during exercise. However, the changes of specific serum miRNAs during Five kilometers’ run test and their association with traditional cardiovascular related indicators have not been well-characterized. A total of 240 venous blood samples were collected before and after the five kilometers’ run test from 120 young subjects. The serum levels of miR-1, miR-21, miR-146a, miR-155, miR-181 and miR-210 were detected by reverse transcription-quantitative PCR (RT-qPCR). Besides, the levels of cardiac Troponin I (cTNI), myoglobin (Myo), creatine kinase (CK), creatine isoenzyme (CK-MB), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), ischemic modified albumin (IMA), interleukin-6 (IL-6), and C-reactive protein (CRP) were also determined by automatic biochemistry analyzer. The correlation between serum miRNAs’ levels and biochemical parameters were also analyzed. The levels of miR-1, miR-146a, miR-155, miR-181 and miR-210 were significantly increased in the serum of the subjects after five kilometers’ run test as compared with resting state. Serum levels of miR-146a, miR-155 and miR-210 after five kilometers’ run test were positively correlated with Myo, CK-MB and LDH respectively, while miR-1, miR-146a, miR-181 and miR-155 were positively correlated with the levels of IL-6. Additionally, all the 5 miRNAs were negatively correlated with IMA levels. Multivariate logistic regression analysis showed that high levels of miR-146a, AST, LDH and IL-6 in serum were risk factors, while low IMA contents was a protective factor for cardiovascular adaptation during exercise. In conclusion, the dynamic changes of miRNAs under the condition of high intensity training contribute to the adaptive regulation of the body's cardiovascular function.