AUTHOR=Pan Chun-Shui , Yan Li , Lin Se-Qi , He Ke , Cui Yuan-Chen , Liu Yu-Ying , Hu Bai-He , Chang Xin , Zhao Xin-Rong , Fan Jing-Yu , Han Jing-Yan 

TITLE=QiShenYiQi Pills Attenuates Ischemia/Reperfusion-Induced Cardiac Microvascular Hyperpermeability Implicating Src/Caveolin-1 and RhoA/ROCK/MLC Signaling

JOURNAL=Frontiers in Physiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.753761

DOI=10.3389/fphys.2021.753761

ISSN=1664-042X

ABSTRACT=<p><bold>Aims:</bold> Coronary microvascular hyperpermeability is an important contributor to ischemia or reperfusion (I/R) injury. However, the effective strategy for this insult remains limited. This study aimed to explore the protective effect of the compound Chinese medicine QiShenYiQi Pills (QSYQ) against coronary microvascular hyperpermeability after cardiac I/R with focusing on the underlying mechanism.</p><p><bold>Methods and Results:</bold> Male Sprague-Dawley rats under anesthesia were subjected to occlusion of left coronary anterior descending artery followed by reperfusion. QSYQ was administrated 90 min before ischemia initiation. Human cardiac microvascular endothelial cells (HCMECs) underwent hypoxia or reoxygenation (H/R) challenge with QSYQ administrated 1 h prior to hypoxia. QSYQ exhibited effects on attenuating microvascular damage and albumin leakage after I/R injury, showing a role in maintaining endothelial junctions, caveolae, and collagen in basement membrane (BM) of microvessels. Study using HCMECs disclosed that QSYQ protected endothelial barrier from impairment by H/R, attenuating the decline of respiratory chain complex I and ATP synthase, activation of Src/caveolin-1 and increase of RhoA/ROCK/p-MLC, MMP-9, and CTSS. PP2, a Src inhibitor, partially imitated the effect of QSYQ.</p><p><bold>Conclusions:</bold> The QSYQ was able to prevent I/R-induced cardiac microvascular hyperpermeability <italic>via</italic> a mechanism involving Src/caveolin-1 and RhoA/ROCK/MLC signaling.</p>