AUTHOR=Sun Lili , Ma Heting , Gao Yuan , Wang Zhiying , Cao Chuanwang 

TITLE=Functional Identification and Characterization of Leucokinin and Its Receptor in the Fall Webworm, Hyphantria cunea

JOURNAL=Frontiers in Physiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.741362

DOI=10.3389/fphys.2021.741362

ISSN=1664-042X

ABSTRACT=<p>Neuropeptides function as central neuromodulators and circulating hormones that modulate insect behavior and physiology. Leucokinin (LK) is an intercellular signaling molecule that mediates many physiological and behavioral processes. However, the functions of LK associated with environmental stress and feeding behavior in the fall webworm, <italic>Hyphantria cunea</italic>, is little known. Our primary objective is to understand the function of LK and LK receptor (LKR) neuroendocrine system in <italic>H. cunea</italic>. In the present study, the results showed that LK/LKR are expressed at different developmental stages and in various tissues of <italic>H. cunea</italic>. A candidate receptor–ligand pairing for LK was identified in the larval transcriptome of <italic>H. cunea</italic>. In a heterologous expression system, the calcium assay was used to demonstrate that LKR is activated by HcLKs in a dose-dependent manner, with 50% effective concentration (EC<sub>50</sub>) values of 8.44–90.44nM. Knockdown of <italic>HcLK</italic> and <italic>HcLKR</italic> by microinjecting target-specific dsRNA leads to several effects in <italic>H. cunea</italic>, including feeding promotion, increase in resistance to desiccation and starvation stress, and regulation of water homeostasis. The transcript levels of <italic>HILP2</italic> (except in the <italic>LK</italic> knockdown group), <italic>HILP5</italic>, and <italic>HILP8</italic> increased, whereas those of <italic>HILP3</italic>, <italic>HILP4</italic>, and <italic>HILP6</italic> decreased; <italic>HILP1</italic>, <italic>HILP2</italic> (in the LK knockdown group), and <italic>HILP7</italic> gene expression was not influenced after <italic>LK</italic> and <italic>LKR</italic> knockdown. Variations in mRNA expression levels in insulin-like peptide genes in the knockdown larvae suggest an essential role of these genes in survival in <italic>H. cunea</italic>. To our knowledge, the present study is the first comprehensive study of LK and LKR – from gene to behavior – in <italic>H. cunea</italic>.</p>