AUTHOR=Maiello Lorenzo , Ball Lorenzo , Micali Marco , Iannuzzi Francesca , Scherf Nico , Hoffmann Ralf-Thorsten , Gama de Abreu Marcelo , Pelosi Paolo , Huhle Robert 

TITLE=Automatic Lung Segmentation and Quantification of Aeration in Computed Tomography of the Chest Using 3D Transfer Learning

JOURNAL=Frontiers in Physiology

VOLUME=12

YEAR=2022

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.725865

DOI=10.3389/fphys.2021.725865

ISSN=1664-042X

ABSTRACT=<sec><title>Background</title><p>Identification of lung parenchyma on computer tomographic (CT) scans in the research setting is done semi-automatically and requires cumbersome manual correction. This is especially true in pathological conditions, hindering the clinical application of aeration compartment (AC) analysis. Deep learning based algorithms have lately been shown to be reliable and time-efficient in segmenting pathologic lungs. In this contribution, we thus propose a novel 3D transfer learning based approach to quantify lung volumes, aeration compartments and lung recruitability.</p></sec><sec><title>Methods</title><p>Two convolutional neural networks developed for biomedical image segmentation (uNet), with different resolutions and fields of view, were implemented using Matlab. Training and evaluation was done on 180 scans of 18 pigs in experimental ARDS (<italic>u</italic>2<italic>Net</italic><sub><italic>Pig</italic></sub>) and on a clinical data set of 150 scans from 58 ICU patients with lung conditions varying from healthy, to COPD, to ARDS and COVID-19 (<italic>u</italic>2<italic>Net</italic><sub><italic>Human</italic></sub>). One manual segmentations (MS) was available for each scan, being a consensus by two experts. Transfer learning was then applied to train <italic>u</italic>2<italic>Net</italic><sub><italic>Pig</italic></sub> on the clinical data set generating <italic>u</italic>2<italic>Net</italic><sub><italic>Transfer</italic></sub>. General segmentation quality was quantified using the Jaccard index (<italic>JI</italic>) and the Boundary Function score (<italic>BF</italic>). The slope between <italic>JI</italic> or <italic>BF</italic> and relative volume of non-aerated compartment (<italic>S</italic><sub><italic>JI</italic></sub> and <italic>S</italic><sub><italic>BF</italic></sub>, respectively) was calculated over data sets to assess robustness toward non-aerated lung regions. Additionally, the relative volume of ACs and lung volumes (LV) were compared between automatic and MS.</p></sec><sec><title>Results</title><p>On the experimental data set, <italic>u</italic>2<italic>Net</italic><sub><italic>Pig</italic></sub> resulted in <italic>JI</italic> = 0.892 [0.88 : 091] (median [inter-quartile range]), <italic>BF</italic> = 0.995 [0.98 : 1.0] and slopes <italic>S</italic><sub><italic>JI</italic></sub> = −0.2 {95% conf. int. −0.23 : −0.16} and <italic>S</italic><sub><italic>BF</italic></sub> = −0.1 {−0.5 : −0.06}. <italic>u</italic>2<italic>Net</italic><sub><italic>Human</italic></sub> showed similar performance compared to <italic>u</italic>2<italic>Net</italic><sub><italic>Pig</italic></sub> in <italic>JI</italic>, <italic>BF</italic> but with reduced robustness <italic>S</italic><sub><italic>JI</italic></sub> = −0.29 {−0.36 : −0.22} and <italic>S</italic><sub><italic>BF</italic></sub> = −0.43 {−0.54 : −0.31}. Transfer learning improved overall <italic>JI</italic> = 0.92 [0.88 : 0.94], <italic>P</italic> &lt; 0.001, but reduced robustness <italic>S</italic><sub><italic>JI</italic></sub> = −0.46 {−0.52 : −0.40}, and affected neither <italic>BF</italic> = 0.96 [0.91 : 0.98] nor <italic>S</italic><sub><italic>BF</italic></sub> = −0.48 {−0.59 : −0.36}. <italic>u</italic>2<italic>Net</italic><sub><italic>Transfer</italic></sub> improved <italic>JI</italic> compared to <italic>u</italic>2<italic>Net</italic><sub><italic>Human</italic></sub> in segmenting healthy (<italic>P</italic> = 0.008), ARDS (<italic>P</italic> &lt; 0.001) and COPD (<italic>P</italic> = 0.004) patients but not in COVID-19 patients (<italic>P</italic> = 0.298). ACs and LV determined using <italic>u</italic>2<italic>Net</italic><sub><italic>Transfer</italic></sub> segmentations exhibited &lt; 5% volume difference compared to MS.</p></sec><sec><title>Conclusion</title><p>Compared to manual segmentations, automatic uNet based 3D lung segmentation provides acceptable quality for both clinical and scientific purposes in the quantification of lung volumes, aeration compartments, and recruitability.</p></sec>