Rubén Mollá-Albaladejo
Juan A. Sánchez-Alcañiz*- Instituto de Neurociencias, UMH&CSIC, San Juan de Alicante, Spain
Among individuals, behavioral differences result from the well-known interplay of nature and nurture. Minute differences in the genetic code can lead to differential gene expression and function, dramatically affecting developmental processes and adult behavior. Environmental factors, epigenetic modifications, and gene expression and function are responsible for generating stochastic behaviors. In the last decade, the advent of high-throughput sequencing has facilitated studying the genetic basis of behavior and individuality. We can now study the genomes of multiple individuals and infer which genetic variations might be responsible for the observed behavior. In addition, the development of high-throughput behavioral paradigms, where multiple isogenic animals can be analyzed in various environmental conditions, has again facilitated the study of the influence of genetic and environmental variations in animal personality. Mainly, Drosophila melanogaster has been the focus of a great effort to understand how inter-individual behavioral differences emerge. The possibility of using large numbers of animals, isogenic populations, and the possibility of modifying neuronal function has made it an ideal model to search for the origins of individuality. In the present review, we will focus on the recent findings that try to shed light on the emergence of individuality with a particular interest in D. melanogaster.
1. Introduction
Individuality, temperament, behavioral syndromes, or animal personality are terms used to define the display of specific behavioral traits that are stable over time (Dall et al., 2004; Sih et al., 2004; Bell, 2007). At the population level, animals tend to show homogeneous behavior. However, if analyzed in more detail, it is clear that individuals within a group show behavioral patterns that differentiate them from the average. For example, in humans, food perception is highly personal, and it depends on the combination of both sociocultural experience and genetic polymorphisms that affect the function of gustatory (Kim et al., 2003) and olfactory receptors (Wysocki and Beauchamp, 1984; Kowalewski and Ray, 2020). This interindividual variation is not exclusive to humans and is generally observed in all living beings. For example, bacteria grown in the laboratory display variations in swimming behavior due to changes in gene expression, indicating that even in populations with the same genetic background and grown in similar conditions, heterogeneous behaviors can be observed (Davidson and Surette, 2008). Experiments with the clonal fish Poecilia formosa show that individuals grown in standardized conditions in isolation after birth display considerable differences in their behavior (Bierbach et al., 2017). Those results would suggest that stochastic developmental events lead to high variability in behavior. For example, variations in mushroom body size in D. melanogaster, affecting aggression, lifespan, and sleep, have been linked with polymorphisms in more than 100 genes (Zwarts et al., 2015). It is important to remark that those variations in behavior are consistent over time. We are not referring to just an acute change in their behavioral pattern or the minor variations resulting from the “noise” in the system that might induce temporary changes in a particular behavior (Faisal et al., 2008). Other stochastic events, inherent to any biological system, such as changes in gene expression or development, will have a more profound impact on the outcome of the behavior, contributing to persistent variations in behavior and the emergence of individuality (Honegger and de Bivort, 2018).
The genetic background of the organism and the developmental history of an individual dramatically affect how the animal will express this individuality (Dall et al., 2004; Sih et al., 2004; Wolf and Weissing, 2010). Although animals of the same species share the same genome, subtle changes during development (i.e., axon guidance) can have severe effects on the final connectivity of the neurons due to stochastic events altering specific behaviors (Linneweber et al., 2020; Kiral et al., 2021). In addition, environmental factors during growth and epigenetic changes will modify gene expression. It is important to remark that although animal personality defines the animal and shows specific stability, it has certain levels of plasticity, as happens with foraging behaviors on a day-to-day basis (Anreiter and Sokolowski, 2019). Previous experiences, growth and developmental conditions, and epigenetic factors form a complex milieu where behavior and individual differences emerge.
D. melanogaster is an outstanding model to study behavior individuality for several reasons. For example, it is possible to dispose of a large number of individuals to analyze per experiment; there is an extensive collection of isogenic lines available with sequenced genomes, and we can manipulate flies at the genetic level (Casillas and Barbadilla, 2017). Moreover, with only 100,000 neurons in the central brain (Raji and Potter, 2021) and a large collection of tools to manipulate neural circuits, D. melanogaster is an excellent model system to understand the genetic and neural basis of behavior heterogeneity.
The present review presents the scientific advances in the study of behavior individuality in D. melanogaster. We will cover the knowledge gained in genetics, development, epigenetics, and the methods used to study individual behavior in flies.
2. Genetic Basis of Animal Individuality
Animal behavior and, ultimately, animal personality results from the interaction of genetic and non-genetic factors. Indeed, animals combine genetic and environmental traits to promote specific adaptation to available resources from the environment and their gene expression adapts to the circumstances (Figure 1A) (Honegger and de Bivort, 2018; Koyama et al., 2020). However, neither nature nor nurture in isolation can explain how and why animals behave the way they do, as each of the two components has its weight. Even more, the expression of specific genes is not constant over time but can change through the life course of the animal, causing modifications in the personality of an individual (Juneja et al., 2016; Lin et al., 2016). Hence, genes are in constant communication with non-genetic factors coupling complex and sensitive networks that will finally define the personality of an individual in response to natural pressure.
Figure 1. (A) Cumulative and relative contribution of different sources of interindividual variability from early development stages to adult life experience. Individuality emerges from the combination of genetic factors, environmental factors, and stochastic factors. (B) Variance distribution for a given phenotypic trait evoking variability among individuals within a population (Buchanan et al., 2015). (C) Variance distribution of phenotypic handedness is inherited to next generations within a population (Ayroles et al., 2015).
The advent of genomics during the last decades has revolutionized the field of behavioral neuroscience, providing evidence on how genes can face natural dynamic variation between individuals and affect different behavioral outputs contributing to population performance (Jin et al., 2001; Ueno and Takahashi, 2020). How, when, and where a gene or genes are expressed and modulate brain activity and processing, could explain why two individuals from the same species, similar genetic backgrounds, and raised in similar conditions behave differently. For example, in D. melanogaster genetic variation in olfactory receptors (Or22a/Or22b, Or35a, and Or47a) affect different odor guidance perceptions among individuals of the same population (Richgels and Rollmann, 2011). Also, variation in odor guidance to 2,3-butanedione showed genes associated with neural development and the later processing in the central nervous system which might be related to that behavioral variation (Brown et al., 2013). This genotypic variation is seen not only at the behavioral level but also, for example, in lifespan or morphological and anatomical traits as brain, wing, thorax, or eye size (Carreira et al., 2016; Buchberger et al., 2021).
In behavioral neuroscience (and neuroscience in general), the use of inbred lines can reduce phenotypic variability. However, even in highly controlled experimental conditions, high levels of variability can be observed both in mice and D. melanogaster (Honegger and de Bivort, 2018). Many animals available and short reproductive time made it possible to generate numerous collections of isogenic lines. The Drosophila Genetic Reference Panel (DGRP) are a set of fly populations derived from subsequent inbred generations producing lines with sequenced genomes and all its polymorphisms annotated (Mackay et al., 2012). As the genome of each of those fly lines is sequenced, it is possible to test the variations in behavior for each line to later search for the genetic basis responsible for such behavior. Also, studies in simple stereotyped behaviors have found complex genetic architectures involved in the final output performance of specific behaviors that differ between individuals. Thirteen genes are involved in the flight performance in D. melanogaster where the variation in the expression and regulation of these genes may reflect the variation in the flight performance among individuals. Among them, polymorphisms at the regulatory region of the transmembrane tyrosine kinase, Egfr, showed the largest behavioral variation affecting wing shape (Spierer et al., 2021). Other studies have used DGRP lines to search genetic variation in aggressive behavior, virgin egg retention, or immune response against pathogens (i.e., Coxiella burnetii) (Akhund-Zade et al., 2017; Guzman et al., 2021). Interestingly, interindividual variation can also be observed in D. melanogaster mating behavior. During courtship, male flies execute a series of stereotyped and progressive behaviors that culminate in mating (Hall, 1994). Even in behavior as stereotyped as courtship, which must avoid interspecies mating, studies made in natural populations have observed variability in male courtship behaviors toward mated females (Ruedi and Hughes, 2008). Genome-Wide Association Studies (GWAS) performed in the inbred collection of DGRP flies showed the influence of genetic variation in courtship variability. Particularly, the transition from copulation to no engagement was associated with SNPs in Serrate and Furin-1 genes (Gaertner et al., 2015).
A key question in behavior individuality is why animals show this range of erratic behaviors. A possible explanation derives from the natural variability in the surrounding environment. A genetically uniform population might be desirable in a stable environment with no threats, as individuals diverting from the average might not be well-adapted. However, in an ever-changing environment, animals must develop strategies to survive. One option would be phenotypic plasticity, where individuals have developed the ability to change their behavior upon environmental requirements. For example, fruit flies adapt their diet to environmental temperature. D. melanogaster feeds primarily from yeast, but if the temperature drops below 15°C, flies change to a plant-based diet. Plants provide the flies with unsaturated fatty acids, increasing cell membrane fluidity and lifespan (Brankatschk et al., 2018). Phenotypic plasticity can also be observed in overwintering Drosophila, winter morphs, where flies display different phenotypic plasticity to adapt to low temperatures (Panel et al., 2020; Stockton et al., 2020).
Another possibility is to hedge their options or diversified bet-hedging. In this scenario, a single genotype produces a distribution of phenotypes, assuring that at least some individuals within the population will be well-adapted to cope with any environmental change (Honegger and de Bivort, 2018). Under those circumstances, individuals show heterogeneous natural behavior. This natural variability would be heritable, causing the behavioral individuality observed. Experiments using wild-type and inbreed lines exploring the animal idiosyncrasies have studied the mechanistic behind animal handedness or better performance using left or right hand. This behavior can be observed in D. melanogaster when it is forced to choose to go left or right in an arena with no other stimulus. In this paradigm, flies showed considerable variability in this particular trait, which relates to specific genotypes (Ayroles et al., 2015; Buchanan et al., 2015). The authors showed that although each population averaged a 50% chance of turning either right or left, some were more variable, with more individuals either turning left or right (Figure 1B). Thus, the turning bias of individual flies was not heritable but was the degree of variability of the population. Furthermore, crossing two “righty” or two “lefty” individuals did not produce hybrids all “righty” and “lefty,” respectively, as the F1 progeny would show average turn vias of 50%. However, the variability of the particular line would be inherited (Figure 1C). A gene encoding an axon guidance molecule, Tenascin-a, has been proposed as a candidate involved in the observed behavior heterogeneity (refer to next section) (Ayroles et al., 2015; Buchanan et al., 2015). This distribution of phenotypes observed might be an evolutionary strategy, diversified bet-hedging, to guarantee that at least some individuals will be well-adapted when facing unpredictable environments. Bet-hedging could be the possible source of variation in the phototactic behavior of flies in two populations of flies from two different climates. Flies from very stable tropical regions where day/light time is relatively stable would show less variability in a phototactic choice assay than the ones from a nordic region, where seasonal changes in light/dark are more dramatic. Serotonin variation among the populations could be the source of such variation as feeding flies with serotonin would decrease variability (Kain et al., 2012; Krams et al., 2021). To reinforce this idea, other studies focused on other species such as Caenorhabditis elegans show that in isogenic sibling individuals raised under the same conditions, serotonin might regulate behavioral variability. Complete depletion of serotonin (tph-1) or some of the G-protein coupled receptors (SER-1, SER-4, and SER-7) induce changes in the individual roaming behavior across development (Stern et al., 2017).
As we have seen, some of those genes affect the development of specific neural circuits. In contrast, others affect neuromodulatory networks, such as serotonin in locomotor behavior in C. elegans. In other cases, mutations in particular alleles affect particular gene regulatory networks, ultimately affecting neuronal function. For example, it is the case of the chaperone heat shock protein 90 (HSP90), involved in the folding and maturation of other proteins. HSP90 mutants show high levels of interindividual morphology variation (Rutherford and Lindquist, 1998). In addition, recent studies have shown that HSP90 mutant flies display a high interindividual variation in circadian motor control (Hung et al., 2009). Daily cycles of light and darkness can entrain the circadian clock. Once established, a gene network can keep it oscillating without environmental cues (Williams and Sehgal, 2001). While wild-type flies showed low variations in their rhythmic activity, flies with decreased activity of the chaperone HSP90 showed variation between individuals, from rhythmic and arrhythmic to other complex behaviors. Those results indicated that HSP90 could be acting as a capacitor of behavior individuality, affecting the degree of variation in circadian behavioral activity (Hung et al., 2009).
All these studies support the idea that in flies from the same population, there is an accumulation of polymorphisms due to spontaneous mutations, natural pressure, or simple genomic diversification from the average of the population, conferring different behavioral personalities among them. Consistent individual differences can result from intra-genotypic variations among individuals and differences in the value of state variables such as metabolic rate, growth rate, or energetic reserves (Amat et al., 2018). Also, stochastic gene expression may underlie the phenomenon of partial penetrance of mutations and variability that may interfere in individual personality (Topalidou and Chalfie, 2011).
3. Developmental and Growth Conditions Shape Animal Personality
In the previous section, we have discussed the genetic basis for behavioral variability. However, we also mentioned the critical role of the developmental process and growth in individual behaviors. We refer to the variations of behavior that are non-genetic as intragenotypic variation. This variability derives from stochastic microenvironmental effects such as temperature, isolation, or food sources that force individuals to adapt phenotypically to the environment (Becher et al., 2010).
Temperature is a wide-ranging environmental factor that flies can experience and must manage to maintain their homeostasis. In D. melanogaster, the life cycle takes longer at low temperatures and accumulates more fat energy stores as a mechanism to cope with possible future starvation periods (Klepsatel et al., 2019, 2020). Previous studies have shown that there is gene expression variation in response to low temperatures in D. melanogaster due to plasticity phenomena (Fry, 2008). Whole-genome sequencing in fly populations evolved in different temperatures has revealed the role of different genes in the recombination rate divergence between populations (Winbush and Singh, 2021). The transcription factors chimo and eve show different levels of expression between flies reared at different temperatures (25 vs. 17°C). This variation modifies the arborization of sensory neurons inducing interindividual variability perceiving temperature (Alpert et al., 2020; Huang et al., 2020). Those temperature changes affect synaptic connectivity in the D. melanogaster visual system, as flies grown at low temperatures (19°C) have more synapse numbers than the ones grown at higher temperatures (25°C) (Kiral et al., 2021). Furthermore, phenotypic plasticity in front of temperature variation is not exclusive of drosophilids as other social insects as honey bees show different learning abilities related to labors within the colony depending on the larvae developmental temperature (Tautz et al., 2003; Jeanson, 2019). Those results indicate that temperature is a major source of phenotypic plasticity and interindividual variability within a population.
Environmental factors can dramatically influence the development of the animal and condition its growth, modifying its behavior. Flies raised in stimulating naturalistic environment vials vs. vials without any enrichment that could match natural environments showed significant differences in fitness. Enriched populations showed higher intragenotypic variability for most of the behavioral traits measured, concluding that enrichment stimuli environment is one of the central sources of variability for behavior traits crucial to surviving (Akhund-Zade et al., 2019). Also, gene expression noise varies depending on the specific gene function, suggesting that variance in gene expression noise in order to evoke phenotypic plasticity may be beneficial for survival to environmental changes (Blake et al., 2006; Newman et al., 2006; Viney and Reece, 2013).
Even in conditions where genetic background and environment are kept constant, similar individuals can develop non-heritable idiosyncratic behaviors, morphology, and gene expression profiles evoking variability that could be consistent with development and life. Stochastic development wiring or minute differences in growth conditions can contribute to the trait under study. Identical populations of pea aphids and flies grown in identical environmental conditions display heterogeneous behaviors, eliminating the role of any internal factor (Schuett et al., 2011; Kain et al., 2012; Ayroles et al., 2015). Therefore, the role of those non-heritable traits in brain development and, therefore, in individual behavior is gaining importance in neuroscience. Studies carried on the visual orientation behavior in D. melanogaster showed that the Dorsal Cluster Neurons axonal projections within the medulla brain are a predictor of visual orientation, suggesting that stochastic variation in brain wiring evoke non-heritable behavioral variations (Linneweber et al., 2020). We mentioned in the previous section that different populations of flies showed variations in their handedness behavior. Tenascin-a encodes a cell surface protein involved in axon guidance and synaptogenesis. GWAS studies of the DGRP lines showed that this protein participates in the wiring of the neural circuits involved in locomotor behavior. Presumably, variations in the protein function might affect the synaptic connectivity of the neurons in the Central Complex of the fly brain, creating the high individual to individual variations, which ultimately will affect the apparent random choice, left or right, creating a bias in specific individuals (Ayroles et al., 2015; Buchanan et al., 2015). These studies indicate how intricate the relation between genes and environment is, showing that the genetic background of a population would determine the observed variability level, becoming heritable. However, the stochastic neuronal wiring in individuals can also be the source of particular behaviors.
These findings support the idea that stochastic variation in brain wiring and gene expression combined with different genetic traits are determinants of such behavior variability.
4. How Epigenetics Influence Behavior
Epigenetics involve any biological mechanisms that regulate the expression of genes without changing the DNA sequences, becoming the crossroad between the genetic and the environmental factors leading to a biological impact upon gene expression (Heard and Martienssen, 2014; Schuebel et al., 2016; Schiele and Domschke, 2018). Different factors influence epigenetic modifications such as diet, experience, characteristics of the ecosystem, lifestyle, and the physiological state of the individuals, impacting on disease outcome, social organization, and individual behavior, among others (Waterland and Jirtle, 2003; Cunliffe, 2016; Dawson et al., 2018; Baenas and Wagner, 2019).
Epigenetic modifications affect animals at the individual and social level, modifying the role of individuals inside specific social contexts (Anreiter et al., 2017; Sara et al., 2019). For example, honey bees display DNA methylation after intruders encounter, leading to aggressive behavior (Herb et al., 2018). Differential histone 3 (H3) acetylation (H3K27) affects morphologically and behaviorally Camponotus floridanus ant workers. Those modifications induce differences in foraging and scouting behaviors leading to high levels of task distribution (Simola et al., 2013, 2016; Yan et al., 2014). Epigenetic modifications also alter parasocial insects like the fruit fly behavioral, developmental, and physiological traits. For example, a low-protein diet induces H3K27 heterochromatin trimethylation shortening the lifespan of flies. In addition, acetylation of H3K27 by blocking the Drosophila Polycomb gene induces a dysregulation of the repression of homeotic genes (Tie et al., 2009, 2016). Furthermore, epigenetic regulation affects foraging behavior by histone methylation of the for (foraging) gene promoter pr4 establishing a polymorphism between sitters and rovers behaviors in D. melanogaster (Anreiter et al., 2017). Other studies showed that euchromatin histone methyltransferase activity affects non-associative learning and courtship memory in Drosophila (Kramer et al., 2011).
5. Individuality in Collective Behavior
Animals coordinate their behavior with other individuals for benefits, including increased opportunities to mate, greater migratory and foraging efficiency, less chance of being attacked, and better energy costs (Handegard et al., 2012; Berdahl et al., 2013; Jolles et al., 2019). Several researchers have focused their research on the study of the neurogenetic bases of collective behavior in order to understand how individuals can form complex social networks among themselves, improving their survival as occurs in social animals like fishes, ducks, bees, or flies (Becher et al., 2010; Bialek et al., 2014; Ramdya et al., 2017).
Behavior individuality within a colony can emerge from self-organization and social interactions benefiting host hospitalization and decision-making processes (Jeanson, 2019). Individual roles within an animal social network can change over time as an evolutionary method to decrease disease transmission (Stroeymeyt et al., 2018). In other cases, biological roles can be persistent for each individual, such as birds taking turns as alarm-calling sentinels in the colony or the task distribution in ant colonies, respectively (Nagy et al., 2010; Yan et al., 2014; Ramdya et al., 2017; Friedman et al., 2020). Individuals in social networks experience social encounters to spread information from informed to uninformed to transmit beneficial information for survival and relevant future decision-making processes (Canright and Engø-Monsen, 2006). Different studies revealed that fruit flies coordinate their oviposition sites based on the information shared by experienced flies through social encounters. Those experiments suggest that highly clustered flies show a high potential to spread information among individuals (Pasquaretta et al., 2016). Besides, flies are aware of the number of individuals and adjust their interactive behavior to the group size (Rooke et al., 2020). Other studies have shown that collective aggregation depends on external stimuli. For example, the Poxn transcription factor and Orco co-receptor are involved in the chemical detection of fly cuticle hydrocarbon pheromones that may be involved in clustering mechanisms (Schneider et al., 2012b). In addition, the mechanoreceptor NompC is involved in collective behavior as NompC mutant flies only avoid noxious CO2 when are clustered with wild-type flies compared with isolated NompC mutant ones. These results indicate that there is spread of information from wild type flies to NompC mutants (Ramdya et al., 2015). These studies, in addition to others, support the idea that fruit flies integrate sensory information in order to drive appropriate collective behavior and facilitate social learning and foraging decisions in larvae and adulthood to buffer efficiently environmental stress (Tinette et al., 2004; Billen, 2006; Lihoreau et al., 2016; Dombrovski et al., 2017; Jolles et al., 2017; Jiang et al., 2020).
Despite all the benefits derived from the establishment of social networks within a group of individuals, D. melanogaster has a parasocial organization where collective and individual behavior remains cohesive. Each group member behaving differently could explain that the cascade of group motion likely emerges from specific individual patterns of behavior (Rosenthal et al., 2015). Nevertheless, there is no evidence that some individuals act as leaders beginning the clustering within groups of fruit flies. However, the aggregation process grows as more flies join the pioneer ones, affecting information spreading (Jiang et al., 2020).
Even if D. melanogaster organization does not fit in a eusocial pattern where there is specific and hierarchical task distribution, the presence of individual behavior heterogeneity may drive crucial collective behavior beneficial for both the individual and the conspecifics individuals. Thus, understanding the dynamics of collective behavior in the fruit fly may guide understanding the neurogenetic bases involved and how the behavioral patterns of animal societies arise.
6. Methods to Study Behavior Individuality
D. melanogaster has emerged as an excellent model to study behavior individuality for several reasons: the small size and short breeding time allow us to obtain large quantities of animals to test in small setups in short periods of time; there is a large number of inbred lines with sequenced and annotated genomes to search for the possible genetic basis of individuality, and; finally, the possibility to manipulate the genome and neurons of the flies allow us to test ultimately how the candidate genes (and neural circuits) affect the generation of behavioral variation (Venken et al., 2011). The number of behavioral paradigms developed to study Drosophila (and in general animal behavior) have blossomed in the last years due to an increase in computer capacity and the development of machine learning algorithms dedicated to it. In Figure 2, we describe some hardware (with custom associated software) used to study behavior in individual flies. For example, the classic Y-maze, where flies can choose between two paths, is scaled up to allow multiple simultaneous recordings of individual flies. With this high-throughput system, the behavior of 25,000 individuals was analyzed and permitted the study of the neural and genetic basis of handedness in flies, identifying candidate genes and neurons (Buchanan et al., 2015) (Figure 2A). As mentioned in previous sections, animals, and particularly D. melanogaster display idiosyncratic behavioral responses to odors. To study this behavior, Honegger et al. build a paradigm arena where individual flies could choose between two odors emanating from opposing ends of a corridor. By video tracking the fly behavior, it was possible to show how neuromodulation was involved in the preferential choice of individuals (Figure 2B) (Honegger et al., 2019). Other innate behaviors like object orientation responses can be analyzed in a high-throughput manner using multiple Buridian paradigm arenas and video tracking (Figure 2C). Using this set up, the authors demonstrated that stochastic developmental events were altering the Dorsal Cluster Neuron circuits of different individuals, leading to idiosyncratic behaviors in flies (Linneweber et al., 2020). Finally, it is also possible to study feeding in D. melanogaster. FlyPad is an automated high-throughput method to study fly ingestion in individual flies. This system would allow the analysis of behavior individuality in, for example, the ability of flies to choose between two types of foods (Itskov et al., 2014) (Figure 2D). Recently, an upgrade in FlyPad named OptoPad allows optogenetically modifying the activity of selected circuits in real-time by ectopically expressing channelrhodopsins in those neurons. With this method, it is possible to couple the feeding activity of the fly with the modification of the neural activity in a closed-loop manner (Moreira et al., 2019).
Figure 2. High throughput analysis of behavior variance. (A) Schematic set up for study locomotor handedness variability by Y-maze (Ayroles et al., 2015; Buchanan et al., 2015). (B) Schematic setup for study olfactory guidance variability. Adapted from Honegger et al. (2019). (C) Schematic Buridian arena to study visual orientation variability (Colomb et al., 2012). (D) Schematic FlyPAD set-up to study feeding microstructure variability (Itskov et al., 2014). (E) Genome-Wide Association Studies (GWAS) workflow.
The previously described methods are focused on the analysis of individual flies. However, the social context is lost, and although flies are non-eusocial insects, flies can aggregate both in vitro (Jiang et al., 2020) and in the wild (Soto-Yéber et al., 2019). The formation of Drosophila clusters is motivated by the presence of mating partners or food, with pheromones like cis-Vaccenyl acetate (Bartelt et al., 1985) and neuromodulators like serotonin (Sun et al., 2020) playing an essential role. As shown in the previous section, individuality can affect collective behavior. For example, within a group, some individuals display “bold” or “shy” behaviors. Newer software, like idtracker.ai, can identify each individual unequivocally in large groups (i.e., up to 70 flies), track overtime their trajectory, and the interaction with other members of the group (Romero-Ferrero et al., 2019). Finally, it is essential to mention that all those methods require standardized procedures, which start with the breeding conditions. Different mediums to homogenize the growth of flies can decrease variability due to external conditions (Piper et al., 2014). Table 1 contains a thorough description of many hardware and software developed in recent years that are applied or can be applied to study behavior individuality. Most of the software and hardware listed are open source and shared with any laboratory that requests its use. We indicate the species for which they were designed or most used. However, researchers can modify the published versions to adapt them to their model system of interest. We apologize in advance for the methods we forgot to mention or did not find, as many appear constantly.
Table 1. Overview of automated and high throughput software and hardware for animal behavior analysis.
After describing the behavior of interest, we need to understand such behavior's genetic and neural basis. The advancement of genomic tools like Next Generation Sequencing, QTL, and GWAS is helping to understand how genes relate to behavioral traits (Bengston et al., 2018) (Figure 1E). Furthermore, we have seen the advantage of working with already sequenced collections of isogenic lines (Mackay et al., 2012). In addition, now we can do experiments to study large natural populations of flies and sequence all individuals with excellent coverage depth at low cost.
Finally, the study of the neural circuitry involved in behavior, D. melanogaster, is an excellent system as many different transgenic lines have been created to label, trace, and analyze complete neural circuits (Jenett et al., 2012). Similar to C. elegans, we aim to map all synaptic partners in the brain of D. melanogaster through electron microscopy (EM). Although we still need to map the whole brain, the connectome for the central brain already exists (Scheffer et al., 2020). So far, those data provide a standalone image of the Drosophila brain. However, it would be desirable to have a full EM reconstruction of each individual's brain analyzed, although this looks right now as an impossible effort. Even for smaller organisms, it is a daunting task, but it could be beneficial as a recent work where EM reconstruction of eight C. elegans brains showed variations in synaptic connectivity between them, making each brain unique (Witvliet et al., 2021). As we learn more about the circuitry involved in particular behaviors, it might be possible in the future to focus our EM reconstruction efforts on small regions of the brain known to control particular behaviors. We could then reconstruct those regions synaptically for multiple individuals, gaining excellent knowledge regarding neural circuit variability between individuals.
7. Conclusions
How do heritable (genetic) and non-heritable (stochastic events) factors interact to shape behavior? It could be possible that individuals carrying particular polymorphisms might be more susceptible to environmental changes, leading to enough variations among individuals to show specific individual persistent behaviors. It would not be easy to differentiate between the genetic and non-genetic basis of such behavior as there is a constant interplay in this particular case. To add more complexity to the problem epigenetic modifications, alter gene function. It means we cannot just focus our efforts on finding particular genetic sequences as the final goal, as we need to understand how genomes change along with the life of an individual. Finally, from a behavioral point of view, we are constantly talking about individuality. At the same time, animals modify their behaviors during their lifetime as they interact with other conspecifics. All this information indicates that the emergence of individuality or animal personality requires the study at different levels.
The latest advancements and development of high-throughput sequencing have finally opened the door to looking for the genetic basis of animal individuality and how the environment affects gene expression. We know individual animals show particular personalities, from flies to mice, monkeys to humans. However, at this very moment, we can start thinking to move from pure ethological studies to the molecular dissection of those behaviors. Neural circuitry tracing and reconstruction through electron microscopy are helping to build a map of the neural connections of the brain. So far, we do not have more than a few individuals. However, understanding and dissecting those circuits might help us finally understand how the expression of particular genes during a particular period or the subtle variations in connectivity could lead to a deeper understanding of individuality.
It is intriguing that nervous systems, like many other biological systems, are plastic within certain boundaries, so we can expect that personal individuality will be expressed deferentially over time or under certain environmental circumstances. D. melanogaster offers an excellent model system as we can test our hypothesis in large groups of animals in a short period of time (Buchanan et al., 2015). In addition, we can control to a large degree the genetic variation of our population by using inbred lines (Ayroles et al., 2015; Linneweber et al., 2020). The generation of the DGRP lines has helped advance this field, as controlling the genetic variation of the populations of interest can help us narrow down the candidate genetic variants, if any, or discard the genetic variation and ascribe it to stochastic developmental processes.
We have focused on the genetic and epigenetic changes that alter individual behavior. We have also studied how stochastic developmental processes alter neural connectivity leading to interindividual variation. However, another possible source of potential behavioral variability might come from the interaction of individuals with environmental microbes, from Wolbachia infections to changes in the gut microbiome. In this particular case, no genetic variation or neural circuit alteration would be responsible for the change in behavior. It is known that Wolbachia infection affects different D. melanogaster behaviors such as sleep (Bi et al., 2018), temperature preference (Truitt et al., 2019), or aggression (Rohrscheib et al., 2015). Alteration in the gut microbiome can affect aggression in Drosophila males (Jia et al., 2021) or sleep and memory (Silva et al., 2021). Those results point to the interaction of individuals with microorganisms as another potential source of interindividual behavior variability that must be taken into consideration.
Finally, from an evolutionary point of view, individuality might play an essential role in providing an adaptative advantage. For example, we have described that animals might use diversified bet-hedging as a mechanism to produce high levels of variation within a population to ensure that at least some individuals will be well-adapted when facing unpredictable environments. Although more experimental evidence accumulates to support this theory, without any doubt, we are in front of a growing field of knowledge that will evolve soon.
Author Contributions
RM-A and JS-A conceived and wrote the manuscript. All authors contributed to the article and approved the submitted version.
Funding
This study was supported by grants from the Generalitat Valenciana, CIDEGENT program (CIDEGENT/2018/035), Spanish Ministry of Science and Innovation (PID2019-105839GA-I00) and Program Ramón y Cajal (RyC2019-026747-I).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher's Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
Acknowledgments
We thank the Generalitat Valenciana and Spanish Ministry of Science for financial support and José María Buil Gómez for critical discussions and comments on the manuscript.
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Keywords: behavior individuality, Drosophila melanogaster, animal personality, neurobiology, stochasticity
Citation: Mollá-Albaladejo R and Sánchez-Alcañiz JA (2021) Behavior Individuality: A Focus on Drosophila melanogaster. Front. Physiol. 12:719038. doi: 10.3389/fphys.2021.719038
Received: 01 June 2021; Accepted: 11 October 2021;
Published: 30 November 2021.
Edited by:
Philippe Lucas, Institut National de la Recherche Agronomique (INRA), FranceReviewed by:
Ralf Stanewsky, University of Münster, GermanyMarko Brankatschk, Technische Universität Dresden, Germany
Copyright © 2021 Mollá-Albaladejo and Sánchez-Alcañiz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Juan A. Sánchez-Alcañiz, anVhbi5zYW5jaGV6JiN4MDAwNDA7dW1oLmVz