AUTHOR=Kong Byungwhi , Khatri Bhuwan , Kang Seong , Shouse Stephanie , Kadhim Hakeem , Kidd Michael , Lassiter Kentu , Hiltz Joseph , Mallmann Barbara , Orlowski Sara , Anthony Nicholas , Bottje Walter , Kuenzel Wayne , Owens Casey TITLE=Blood Plasma Biomarkers for Woody Breast Disease in Commercial Broilers JOURNAL=Frontiers in Physiology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.712694 DOI=10.3389/fphys.2021.712694 ISSN=1664-042X ABSTRACT=
Woody breast (WB) myopathy results in poor muscle quality. The increasing incidence of WB over the last several years indicates a need for improved prediction or early diagnosis. We hypothesized that the use of body fluids, including blood, may be more suitable than breast muscle tissue in developing a minimally invasive diagnostic tool for WB detection. To identify potential early-age-biomarkers that may represent the potential onset of WB, blood samples were collected from 100, 4 wks old commercial male broilers. At 8 wks of age, WB conditions were scored by manual palpation. A total of 32 blood plasma samples (eight for each group of WB and non-WB control birds at two time points, 4 wks and 8 wks) were subjected to shotgun proteomics and untargeted metabolomics to identify differentially abundant plasma proteins and metabolites in WB broilers compared to non-WB control (Con) broilers. From the proteomics assay, 25 and 16 plasma proteins were differentially abundant (