AUTHOR=Moreno-Navarrete José María , Comas Ferran , de Jager Vincent , Fernández-Real José Manuel , Bouma Hjalmar R. 

TITLE=Cecal Ligation and Puncture-Induced Sepsis Promotes Brown Adipose Tissue Inflammation Without Any Impact on Expression of Thermogenic-Related Genes

JOURNAL=Frontiers in Physiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.692618

DOI=10.3389/fphys.2021.692618

ISSN=1664-042X

ABSTRACT=<p><bold>Background and Aims:</bold> The negative effects of chronic low-level inflammation on adipose tissue physiology have been extensively demonstrated, whereas the effects of acute inflammation are less studied. Here, we aimed to investigate the effects of sepsis-induced acute inflammation on gene expression markers of brown and white adipose tissue functionality.</p><p><bold>Methods:</bold> Brown adipose tissue (BAT) and perirenal white adipose tissue (prWAT) gene expression markers were analyzed in cecal ligation and puncture (CLP)-induced sepsis mice model.</p><p><bold>Results:</bold> CLP-induced sepsis attenuated expression of adipogenesis-related genes, in parallel to increased <italic>Tnf, Il6</italic>, and <italic>Ltf</italic> gene expression in prWAT. In contrast, CLP-induced sepsis resulted in increased expression of pro-inflammatory genes (<italic>Il6, Ltf</italic>, and <italic>Lbp</italic>) in BAT, without affecting expression of genes encoding for thermogenic activity.</p><p><bold>Conclusion:</bold> Sepsis promotes both prWAT and BAT inflammation, associated with reduced adipogenesis-related gene expression in prWAT, without significant effects on BAT thermogenic genes.</p>