AUTHOR=Han Hui , Liu Zemin , Yin Jie , Gao Jing , He Liuqin , Wang Chenyu , Hou Ruoxin , He Xingguo , Wang Guoqiang , Li Tiejun , Yin Yulong 

TITLE=D-Galactose Induces Chronic Oxidative Stress and Alters Gut Microbiota in Weaned Piglets

JOURNAL=Frontiers in Physiology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2021.634283

DOI=10.3389/fphys.2021.634283

ISSN=1664-042X

ABSTRACT=<p>Oxidative stress commonly occurs in pig production, which can severely damage the intestinal function of weaned piglets. This study was conducted to investigate the effects of <sc>D</sc>-galactose with different levels used to induce chronic oxidative stress on growth performance, intestinal morphology and gut microbiota in weaned piglets. The results showed that addition of 10 and 20 g/kg BW <sc>D</sc>-galactose reduced average daily gain and average daily feed intake from the first to the third week. 10 g/kg BW <sc>D</sc>-galactose increased the concentration of serum MDA at the second and third week. 10 g/kg BW <sc>D</sc>-galactose significantly influenced the jejunal and ileal expressions of <italic>GPx1</italic>, <italic>CAT1</italic>, and <italic>MnSOD</italic>. The results of 16S rRNA sequencing showed that compared with the control, 10 and 20 g/kg BW <sc>D</sc>-galactose significantly decreased the relative abundance of <italic>Tenericutes</italic>, <italic>Erysipelotrichia</italic>, <italic>Erysipelotrichales</italic>, and <italic>Erysipelotrichaceae</italic>, while increased the relative abundance of <italic>Negativicutes</italic>, <italic>Selenomonnadales</italic>, and <italic>Veillonellaceae</italic>. The results indicated that treatment with 10 g/kg BW/day <sc>D</sc>-galactose for 3 weeks could induce chronic oxidative stress, reduce the growth performance and alter gut microbiota in weaned piglets.</p>