The Torpid State: Recent Advances in Metabolic Adaptations and Protective Mechanisms†

Torpor and hibernation are powerful strategies enabling animals to survive periods of low resource availability. The state of torpor results from an active and drastic reduction of an individual’s metabolic rate (MR) associated with a relatively pronounced decrease in body temperature. To date, several forms of torpor have been described in all three mammalian subclasses, i.e., monotremes, marsupials, and placentals, as well as in a few avian orders. This review highlights some of the characteristics, from the whole organism down to cellular and molecular aspects, associated with the torpor phenotype. The first part of this review focuses on the specific metabolic adaptations of torpor, as it is used by many species from temperate zones. This notably includes the endocrine changes involved in fat- and food-storing hibernating species, explaining biomedical implications of MR depression. We further compare adaptive mechanisms occurring in opportunistic vs. seasonal heterotherms, such as tropical and sub-tropical species. Such comparisons bring new insights into the metabolic origins of hibernation among tropical species, including resistance mechanisms to oxidative stress. The second section of this review emphasizes the mechanisms enabling heterotherms to protect their key organs against potential threats, such as reactive oxygen species, associated with the torpid state. We notably address the mechanisms of cellular rehabilitation and protection during torpor and hibernation, with an emphasis on the brain, a central organ requiring protection during torpor and recovery. Also, a special focus is given to the role of an ubiquitous and readily-diffusing molecule, hydrogen sulfide (H₂S), in protecting against ischemia-reperfusion damage in various organs over the torpor-arousal cycle and during the torpid state. We conclude that (i) the flexibility of torpor use as an adaptive strategy enables different heterothermic species to substantially suppress their energy needs during periods of severely reduced food availability, (ii) the torpor phenotype implies marked metabolic adaptations from the whole organism down to cellular and molecular levels, and (iii) the torpid state is associated with highly efficient rehabilitation and protective mechanisms ensuring the continuity of proper bodily functions. Comparison of mechanisms in monotremes and marsupials is warranted for understanding the origin and evolution of mammalian torpor.

Introduction

Torpor and hibernation represent powerful strategies that enable animals to survive periods of low resource availability in their environment. The state of torpor results from an active and drastic reduction of metabolic rate (MR) associated with an accompanying decrease in body temperature (T_b) after passive cooling (Heldmaier et al., 2004; Jastroch et al., 2016). According to several authors (Ruf and Geiser, 2015; Geiser, 2020), two different forms of torpor, also called heterothermy, can be distinguished based on the duration of the hypometabolic state. In daily torpor, animals show average minimum torpid MR of ∼19% of basal rates and lower their T_b to usually between 12 and 25°C during torpor; torpor lasts less than 24 h and is accompanied by continued foraging (Ruf and Geiser, 2015). On the other hand, during hibernation, individuals achieve a minimum torpid MR of 4% of basal rates, along with a variable reduction of their T_b ranging on average for most species between 0 and 10°C (Ruf and Geiser, 2015). Hence, hibernation corresponds to multiple and successive torpor bouts lasting for days to few weeks, during which animals rely entirely on fuel stores, such as body fat and/or food caches (Humphries et al., 2003a,b; Dark, 2005). Torpor is employed by all three mammalian subclasses, i.e., monotremes, marsupial, and placentals, as well as several avian orders (Ruf and Geiser, 2015) whereas hibernation is documented in mammals from all three subclasses but is known for only one bird species. Daily torpor is diverse in both mammals and birds, and is typically not as seasonal as hibernation (Geiser, 2020). The use of torpor is often associated with species inhabiting cold and seasonal habitats, such as temperate and arctic zones, but torpor is also used by many non-Holarctic species, i.e., the tropics and southern hemisphere (Nowack et al., 2020).

Most seasonal heterothermic eutherian species enter an obligatory physiological and behavioral preparation period when days become shorter, i.e., announcing the winter season. Such remodeling is controlled by the hypothalamus, operates at multiple levels (reviewed in Auld et al., 2010), and comprises a variety of physiological, biochemical, and molecular changes such as regulations of metabolic processes, erythropoiesis, protein transcription, membrane composition, and thermogenesis (Klug and Brigham, 2015; Ruf and Geiser, 2015; Jastroch et al., 2016). This leads to drastic changes in most of biological functions, including metabolic (Jastroch et al., 2016) and reproductive (Clarke and Caraty, 2013) signaling pathways. Actually, the physiological preparations for harsh winter conditions is probably explained by an adjustment of the thermoneutral zone combined with a decrease in basal MR (Kobbe et al., 2014), which contributes to the accumulation of energy body (fat) stores. Additionally, heterothermic species adjust the onset of torpor or hibernation periods to environmental factors, including food availability and/or quality (notably in terms of lipid composition), and T_a (Boyles et al., 2007; Geiser, 2013; Giroud et al., 2018), therefore introducing some flexibility to these syndromes. Further, diverse phenology of hibernation was described among different age classes, i.e., juveniles, yearlings, and adults (Michener, 1978; French, 1990; Kart Gür and Gür, 2015; Siutz et al., 2016), but also in aged individuals compared to younger ones (e.g., Bieber et al., 2018). These differences were mostly linked to ecological cues, including developmental constraints, rather than physiological or molecular mechanistic aspects. For instance, it is known that juveniles, especially those born late in the reproductive season, delay hibernation onset compared to yearlings or adult individuals (Boag and Murie, 1981; Stumpfel et al., 2017), due to their need to further grow and develop and extra time needed to accumulate sufficient fat reserves prior to hibernation (Mahlert et al., 2018); a process that strongly determines the overwintering survival of the individuals. The demonstration that the use of torpor and the physiological remodeling required for the setup of the torpor phenotype is a deeply conserved feature in species living in seasonal environments comes from the variety of studies both from wild and captive conditions. Indeed, organisms maintained under captive environment, with stable and favorable conditions, show persistent circannual rhythms even after more than fifty generations (Perret and Aujard, 2001; Terrien et al., 2017), indicating the deep genetic imprinting of the control of biological clocks on metabolism and reproduction (Dardente et al., 2019).

During the hysteresis of the torpor-arousal cycle over hibernation, the whole organism including organs, tissues, cells, and molecules experience major metabolic transitions triggered by extreme changes in MR and T_b. This is notably the case during the transition phase of arousal from torpor when individual’s MR shows a drastic several-fold increase allowing T_b to return to normothermia. Periodic arousals represent the highest proportion of energy expended during the hibernation process, e.g., 70–80% in temperate species (Wang, 1978), and arousals are accompanied with increases in oxidative stress levels, as supported by substantial shortening of telomere lengths in individuals over winter hibernation (Turbill et al., 2013; Giroud et al., 2014; Hoelzl et al., 2016; Nowack et al., 2019). Prior to hibernation, antioxidant defenses, and other specific regulatory processes are also upregulated to ensure the integrity of body organs, tissues, and cells (for review, see Carey et al., 2003). During the torpid state, specific protective mechanisms enter into play to ensure the continuity of key physiological functions, including heart, lung, muscle, and brain activity, to sustain life at low pace (for reviews, see Johansson, 1996; Ruf and Arnold, 2008; Talaei et al., 2012; Jastroch et al., 2016; Chanon et al., 2018; Nespolo et al., 2018).

Despite the threatening side associated with the torpid state, some progress has been made to further understand the mechanisms underlying the onset, maintenance and arousal from torpor, notably by pharmacological induction of hypometabolism in non-heterothermic species (Bouma et al., 2012). Several molecules and compounds for the induction and the maintenance of a torpor-like state in non-heterotherms have so far received special attention, including 5′-AMP and hydrogen sulfide (H₂S). In particular, the latter seems to be involved in the metabolic maintenance and protective mechanisms of the torpid state in hibernators (see section below for further details). Recently, it has been established that fasting and cold are the most important proximate determinants of torpor in mice, acting by a hypothalamic neuronal circuit as a feedback between fasting, cold, and brown adipose tissue (BAT) activity. Using “designer receptors exclusively activated by designer drugs” (DREADDs) systems, specific hypothalamic neurons were identified and were shown to be involved in the occurrence of either a short-term (daily) torpor (Hrvatin et al., 2020) or a long-term, multi-days hypometabolic state in mice similar to hibernation (Takahashi et al., 2020). Interestingly, a light-sensitive receptor protein expressed in neurons of the preoptic area inhibits BAT metabolism, thus demonstrating a strong link between light and metabolism in animals (Zhang et al., 2020). These results, however, open the question as to how this could work in heterothermic animals with no functional descriptions of BAT, i.e., marsupials and monotremes (Gaudry and Campbell, 2017). One mechanism and its evolutionary implications for heat generation as an alternative to uncoupling processes in BAT could be linked to non-shivering thermogenesis (NST) in muscle (reviewed in Nowack et al., 2017). Recently, this has been demonstrated to occur in wild boar piglets, a species lacking BAT, but which demonstrates marked thermoregulatory adaptive responses (Nowack et al., 2019).

The objective of the present review is then to highlight some of the characteristics associated with the torpor phenotype. In the first section, we focus on specific adaptations for torpor occurring in heterotherms that show contrasting heterothermic patterns. This includes (i) metabolic and endocrine changes involved in fat-storing vs. food-storing hibernating species, and (ii) adaptive mechanisms occurring in opportunistic vs. seasonal heterotherms, such as tropical and sub-tropical species. The latter notably brings, based on recent data on molecular aspects, new insights on the metabolic origins of hibernation among tropical species, and on the view of daily torpor and hibernation as a continuum of hypometabolic responses. In the second section of this review we emphasize the molecular mechanisms enabling heterotherms to protect key organs against potential threats, such as reactive oxygen species, associated with the torpid state. We notably address mechanisms of cellular rehabilitation and protection during torpor and hibernation, with (i) an emphasis on the brain, a central organ to be protected during torpor and recovery after, and (ii) the role of a ubiquitous and readily-diffusing molecule, H₂S, in protecting the integrity of organs against damage occurring over the torpor-arousal cycle and during the torpid state.

Metabolic Adaptations During Torpor and Hibernation

Fat-Storing vs. Food-Storing Hibernators: Hibernating Patterns, Lipid, and Energy Metabolism

Hibernators are generally classified as either (i) fat-storing species, i.e., animals that do not feed during hibernation and rely entirely on body fat reserves accumulated prior to hibernation to cover winter energy requirements, or (ii) food-storing species that feed during periodic arousals between deep torpor bouts and therefore hoard large amounts of food prior to winter (mainly seeds) in their burrow (French, 1988). Due to these different strategies of energy acquisition, fat- and food-storing species show contrasting physiological and behavioral adaptations that we will review in this section.

Fat-storing species typically undergo an intense period of hyperphagia to build up large amounts of internal fat reserves during several weeks to months before hibernation. As example, golden-mantled ground squirrels (Callospermophilus lateralis) show respectively a two and threefold increase in body and fat mass, in only 5–7 weeks prior to hibernation (Kenagy and Barnes, 1988). Yellow-bellied marmots (Marmota flaviventris) also increase their body mass up to 150% through accumulating 3–4.5 kg body fat during their active period in summer (Florant et al., 2004). The accumulation of large internal fat stores associated with long and deep torpor bouts (as long as 15 days), and sometimes social thermoregulation, enable fat-storing animals to fast throughout winter. By contrast, food-storing species store large amounts of food in a burrow. These species are mainly granivorous, because seeds are the only food items that can be stored several months without rotting (Humphries et al., 2003b). Only a few species are food-storing hibernators, mainly hamsters and chipmunks and individuals typically weigh between 10 and 300 g and are solitary. During hibernation, food-storing animals undergo shorter torpor bouts than fat-storing species, but have longer interbout euthermic episodes during which individuals consume their food hoards (Wollnik and Schmidt, 1995; Humphries et al., 2001; Table 1).

TABLE 1

Table 1. Hibernation parameters of the common hamster (Cricetus cricetus), a food-storing species, and the garden dormouse (Eliomys quercinus), a fat-storing species, under controlled conditions of ambient temperature (T_a) and photoperiod.

Metabolic Changes in Fat-Storing Hibernating Species According to Body Fat Mass Changes and Activity

Fat-storing species show an increase in body (fat) mass prior to hibernation (Figures 1A, 3 left panel). Since individuals do not feed during the whole hibernation period, fat-storing hibernators rely on their fat stores to cover their energy requirements during winter. Plasma profiles of these animals during hibernation are characterized by a decrease in triglycerides, whereas plasma levels of ketone bodies increase (Rauch and Behrisch, 1981; Krilowicz, 1985; Nizielski et al., 1989). Lipid use and, more precisely, oxidation of non-esterified fatty acids (NEFAs) during hibernation are also supported by a respiratory quotient (RQ) close to 0.7 during torpor episodes (Lyman and Chatfield, 1955; Mokrasch et al., 1960; Snapp and Heller, 1981). Glycemia is maintained throughout hibernation thanks to the activation of gluconeogenic pathways (Twente and Twente, 1967; Tashima et al., 1970; Krilowicz, 1985; Florant et al., 1986; Nizielski et al., 1989). All these findings were recently confirmed via RNA sequencing and proteomic analyses in hibernating 13-lined ground squirrels (Hampton et al., 2011, 2013; Vermillion et al., 2015).

FIGURE 1

Figure 1. Schematic representation for comparison of body mass changes and use of energy substrates throughout hibernation in fat-storing and food-storing species. (A) In fat-storing hibernators, e.g., marmots, animals undergo a long-term fast during winter by mobilizing mainly lipids, accumulated prior to winter in white adipose tissue, to sustain their energy requirements. This leads to a substantial reduction of body (fat) mass of individuals over their winter hibernation fast. Energy can also come from gluconeogenesis, i.e., the synthesis of glucose from non-carbohydrate substrates during hibernation. (B) In contrast, food-storing hibernators, e.g., Syrian hamsters, experience a state of intermittent fasting during winter hibernation. Animals mobilize lipid stores during the torpor phases, but feed during interbout arousals, using glucose as main source of energy, and partially restore their body lipid/glycogen stores. In contrast to fat-storing species, glycemia decreases during torpor, which could constitute an endogenous signal for food-storing hibernators to arise from torpor and feed during interbout euthermia.

In fat-storing hibernators, the tissue showing the greatest changes during hibernation is probably the white adipose tissue (WAT). Therefore, it was interesting to look at adipokine levels over the seasons. Leptin is secreted proportionally to the amount of WAT. Hence, not surprising, plasma leptin levels are substantially high after fat accumulation, just before hibernation, compared to summer levels (Marmota monax: Concannon et al., 2001; M. flaviventris: Florant et al., 2004; Dromiciops gliroides: Franco et al., 2017; Table 2). However, this hormone is known to increase energy expenditure and to inhibit the initiation of torpor (Freeman et al., 2004). In the woodchuck, a large fat-storing species, it seems that plasma leptin levels and hypothalamic sensitivity to this hormone are uncoupled during the preparatory fattening phase prior to the hibernation onset (Concannon et al., 2001). In small hibernating species, uncoupling also exists, but between adiposity and plasma leptin levels, as shown in the little brown bat (Myotis luciferus: Kronfeld-Schor et al., 2000). Similar patterns have been observed in golden-mantled ground squirrels (C. lateralis: Healy et al., 2008) and arctic ground squirrels (Urocitellus parryii: Ormseth et al., 1996; Boyer et al., 1997). Concerning adiponectin, an adipokine that is secreted inversely proportional to lipid stores (Lafontan and Viguerie, 2006), only one study has assessed its variations during the annual cycle of a hibernating species (Florant et al., 2004). This study conducted in marmots (M. flaviventris) showed that adiponectin is low at the beginning of fat accumulation and elevated during hibernation (Florant et al., 2004). This seems consistent with the role of adiponectin in stimulating fatty acid oxidation (Fruebis et al., 2001; Masaki et al., 2003). Other hormones controlling metabolic pathways have been measured during hibernation compared to the active season in fat-storing species. Pancreatic hormone levels are low during hibernation in deep torpor, but increase during the rewarming phase (Erinaceus europaeus: Hoo-Paris and Sutter, 1980; Hoo-Paris et al., 1982; Glis glis: Castex et al., 1984; Hoo-Paris et al., 1985; C. lateralis: Bauman et al., 1987). Glucagon level remains high during arousals whereas insulin level decreases, probably to sustain glycemia during this phase of euthermia (Hoo-Paris et al., 1985). In larger species, pancreatic hormone levels remain low throughout hibernation (M. flaviventris: Florant et al., 1986). The orexigenic hormone ghrelin increases before hibernation and may stimulate food ingestion during this period of fat accumulation (Citellus lateralis: Healy et al., 2010). Plasma ghrelin concentration is low during hibernation in deep torpor and increases again upon arousal from torpor, as generally observed during stages of starvation (Cummings, 2006). Cortisol decreases during deep torpor and increases again during arousal phases, but stays lower than during the active foraging phase (Popova and Koryakina, 1981; Citellus citellus: Shivatcheva et al., 1988). This might prevent the induction of catabolic pathways, especially of body proteins, during hibernation.

TABLE 2

Table 2. Plasma hormone concentrations (means ± SEM) of fat-storing species during long photoperiod (“LP”), short photoperiod (“SP”) hibernation at temperate (“SP warm”) or low (“SP cold”) ambient temperatures.

Metabolic Changes in Food-Storing Hibernating Species

Contrary to fat-storing hibernators, food-storing species do not gain weight before hibernation, or if they do, to a much lesser extent than fat-storing species. However, food-storing hibernators consume body fat (retroperitoneal and peri-epididymal fat pads) during hibernating bouts, as indicated by RQ close to 0.7 in torpor (Figure 2), and a decrease in triglyceridaemia, whereas plasma NEFA concentration increases (Mesocricetus auratus: Weitten et al., 2013). These NEFAs are mainly converted into acetoacetate. In contrast to fat-storing species, glycemia decreases during deep torpor, which could constitute an endogenous signal for food-storing hibernators to arise from torpor and feed during interbout euthermia. During arousals, the plasma profile of hamsters is characterized by a post-prandial state, with an increase in glycemia and triglyceridaemia and a decrease in plasma concentrations of both NEFAs and ketone bodies (Figure 1B). As described above, in fat-storing species, most hormones are at their nadir during hibernating bouts. This is also the case for food-storing species (Table 3). As an example, pancreatic hormone levels are low during deep torpor in the Syrian hamster (M. auratus) during hibernation but also during the pre-hibernation phase and upon arousal (Weitten et al., 2013). This is surprising because these animals are consuming stored food during arousal phases, leading to changes in plasma metabolite levels and especially glycemia that should trigger changes in pancreatic hormone secretion. However, activity of the pancreas seems to remain low in food-storing species under short photoperiod conditions, which is known to induce a winter-like phenotype in the individuals. The gut regulatory peptides GLP-1 and GIP also decrease significantly under short photoperiod (non-hibernating) conditions and during hibernation in deep torpor, but show a slight increase after food ingestion during arousal phases (Weitten et al., 2013). In fact, these peptides are generally secreted in response to food ingestion to enhance anabolic pathways (Baggio and Drucker, 2007). In food-storing species, a decrease in corticosteronemia during torpor probably prevents body protein catabolism, like in fat-storing species (Weitten et al., 2013). This latter hypothesis may be supported by an absence of increase in uremia during torpor. As in small fat-storing species, leptinemia decreases in the pre-hibernation phase (Weitten et al., 2013), suggesting an uncoupling between adiposity and leptin secretion, probably to induce a torpid state. Also, as in fat-storing animals, plasma adiponectin levels remain high throughout hibernation (Weitten et al., 2013), probably to stimulate NEFA oxidation. Adiponectin is also known to play a role in thermogenesis (Masaki et al., 2003) and could therefore enable food-storing species to maintain T_b at a few degrees above ambient temperature (T_a) during hibernation in deep torpor.

FIGURE 2

Figure 2. Hourly variations in MR (“VO₂”) and respiratory quotient (“RQ”) toward the end of hibernation and after emergence in the common hamster, a food-storing hibernator. During torpor, VO₂ decreases substantially and RQ drops to values close to 0.7, indicating the primary oxidation of lipid to fuel energy requirements at low temperatures. Upon arousals, RQ increases to intermittent values or ∼0.8 (indicating oxidation of mixed substrates), whereas values rise to ∼0.9 at emergence from hibernation and further (0.95) toward the active season. The arrow represents the termination of hibernation, indicating an almost exclusive metabolization of carbohydrates.

TABLE 3

Table 3. Plasma hormone concentrations (means ± SEM) of Syrian hamsters (Mesocricetus auratus) under long photoperiod at 20°C (“LP20”), short photoperiod at 20°C (“SP20”), and short photoperiod at 8°C in torpor (“Torpor”) or during arousal phases (“Arousal”).

Gastro-Intestinal Adaptations of Food-Storing Species

Complete atrophy of the intestinal mucosa occurs in fat-storing species during hibernation (Ictidomys tridecemlineatus: Carey, 1990, 1995); Marmota marmota: Hume et al., 2002). In food-storing species, however, intestinal morphology is preserved and jejunal villi length even increases during torpor, as described in Syrian hamsters (M. auratus; Weitten et al., 2013). Despite mucosal atrophy during hibernation, fat-storing species show a maintenance of the expression of some intestinal enzymes and transporters such as those involved in glucose absorption (Carey and Martin, 1996). Similarly, Galluser et al. (1988) observed that enzymes involved in protein and sugar hydrolysis were expressed throughout hibernation in the common hamster (Cricetus cricetus), a food-storing species. A further study of common hamsters even showed that intestinal hydrolysis of triglycerides, starch, and protein was maintained during hibernation as well as NEFA and glucose absorption (Weitten et al., 2016). Glucose absorption was even increased in hibernating hamsters, certainly to restore glycemia and glycogen stores for the subsequent hibernating bout. In another food-storing species, the eastern chipmunk (Tamias striatus), digestive efficiency was positively correlated to torpor use (Humphries et al., 2001). According to these authors, food-storing species could acquire a twofold advantage in undergoing a torpid state: energy sparing and increased efficiency for assimilating fuel reserves.

Biomedical and Evolutionary Implications of Contrasted Hibernation Strategies: The Case of Fat- vs. Food-Storing Hibernators

One can consider hibernation in fat-storing species as a continuous state of long-term fasting, whereas food-storing hibernators alternate short fasting and feeding bouts, which resembles intermittent fasting. These two types of fasting, i.e., continuous and intermittent, could have major biomedical implications in terms of depression of MR and positive effects of fasting on human health and longevity, as highlighted by a number of studies in this field (for reviews, see Martin et al., 2006; Golbidi et al., 2017; Patterson and Sears, 2017; de Cabo and Mattson, 2019). Indeed, these contrasted responses to metabolic depression and intermittent or long-term fully reduced calorie intake are transduced into specific metabolic adaptations, including the maintenance of a fully-functional digestive tract in food-storing species throughout the hibernating season. Such a strategy should lead to a more positive energy balance in these individuals during hibernation compared to fat-storing species.

Beyond biomedical considerations, what do these contrasted hibernation behaviors imply in terms of evolutionary perspectives? This can be addressed by comparing different life-history traits between fat- and food-storing hibernators. At first, overwintering survival was assumed to be worse in food-storing species because of a risk of rotting or pilfering of food hoards (Davis, 1976; Vander Wall, 1990). However, food-storing animals should have a better body condition upon emergence from hibernation, due to their ability to assimilate food along winter hibernation (Humphries et al., 2001). Therefore, food-storing animals can immediately mate after hibernation, and are able to produce more litters per year compared to fat-storing species. These litters are generally composed of more offspring that grow more rapidly and become fertile earlier (sometimes within their year of birth), leading to a better reproductive success in food-storing than in fat-storing species. However, the maximum lifespan is higher in fat-storing hibernators than in food storing species or non-heterotherms (Wolf et al., 2007; Careau et al., 2009; Turbill et al., 2011; Ruf et al., 2012). Hence, from an evolutionary perspective, the fat-storing strategy might correspond to a slow life history, with a trade-off in favor of survival, whereas the food-storing strategy might be in line with a fast life history, with short life expectancy and a trade-off in favor of reproduction.

Torpor in the Tropics – Opportunistic (Daily) Torpor vs. Seasonal Hibernation

Another case of contrasted use of heterothermy and its ecological and evolutionary implications is highlighted by adaptive mechanisms occurring in opportunistic and seasonal heterotherms. In particular, comparisons of the gray mouse lemur (Microcebus murinus) and the “monito del monte” (D. gliroides) are expected to bring new insights on the metabolic origins of hibernation among tropical and sub-tropical species, and notably based on molecular aspects supporting the view of the existence of a continuum of hypometabolic responses between daily torpor and hibernation.

Two Cases of Opportunistic Heterotherms: Lemurs and Monitos

In this section, we will refer to “hibernation” as a synonym for “seasonal obligatory long-lasting torpor bouts,” occurring in a predictable manner, to distinguish it from “daily opportunistic torpor,” which occurs among species that perform short (few hours) and shallow torpor events (T_b reduction to 15–18°C) (Song and Geiser, 1997; Bartels et al., 1998; Bozinovic et al., 2007), and generally occurs as an adaptation to unpredictable environments (Ruf and Geiser, 2015; Geiser, 2020). These authors maintain that the evolutionary origin and functional meaning of daily opportunistic torpor is different from that of seasonal torpor, and they classify species separately between “daily heterotherms” or “seasonal heterotherms,” but not without debate (Boyles et al., 2013; Nowack et al., 2020). With this criterion, more than 50% of Australian mammal species appear to exhibit some form of heterothermy, showing great physiological diversity, ranging from the long hibernation bouts of echidnas (7 months) to the short torpor bouts of Dasyurid marsupials and bats (Geiser and Körtner, 2010). African heterotherms include Madagascar tenrecs (Echinops telfari) that display seasonal torpor (Lovegrove and Genin, 2008), elephant shrews (Elephantulus) with random patterns of hibernation-daily torpor (Lovegrove et al., 2001), Cheirogaleidae lemurs (Storey, 2015; Faherty et al., 2016) that show daily torpor even at “warm” temperatures, and the mouse lemur (M. murinus) that shows flexible heterothermic phenotypes (Schmid and Ganzhorn, 2009). Daily torpor has been described for a few South American species including hummingbirds (Carpenter, 1974; Wolf et al., 2020), bats (Bozinovic et al., 1985), rodents (Bozinovic and Marquet, 1991), and didelphid marsupials (Bozinovic et al., 2007; Geiser and Martin, 2013). However, seasonal torpor is only known in the Microbiotherian “monito del monte,” D. gliroides (Bozinovic et al., 2004).

Monito del Monte, a Flexible Hibernator

The “monito del monte” (“monitos,” hereafter) is a hibernating South American marsupial (Bozinovic et al., 2004) phylogenetically more related to Australian marsupials than to other South and North American marsupial species (Mitchell et al., 2014). This species is distributed in a latitudinal range of about 1000 km, closely associated with the temperate rainforests of Southern South America (“Valdivian” forests) in Chile and Argentina and covering altitudes ranging from 200 m.a.s.l. in coastal forests to 1600 m.a.s.l. in high Andean Nothofagus pumilio (“lenga”) forests (Martin, 2010; Valladares-Gómez et al., 2019). Hibernation in monitos extends from approximately May to September and animals show a marked seasonality in fat deposits and energy expenditure (Figure 3). They are strictly arboreal (Godoy-Güinao et al., 2018), social and omnivorous with great preference for fruits which make them important dispersers of several native plants (Amico and Aizen, 2000; Amico et al., 2009). Monitos hibernate in clusters of four to nine individuals in elaborate nests that they build using mosses and South American bamboo (Chusquea quila); these potentially represent a strategy for heat conservation in winter (Celis-Diez et al., 2012; Nespolo et al., 2020). Although initially described as seasonal heterotherms (Bozinovic et al., 2004), recent field studies using miniature data loggers for simultaneously recordings of T_a and T_b over the whole hibernation cycle, revealed that monitos can experience long torpor bouts in winter (Figure 4A), but can also undergo short daily torpor episodes in any time of year, even at mild temperatures (T_a = 20°C, see Nespolo et al., 2010). While in deep torpor, monitos defend a critical T_b of about 5°C and thermoregulate in torpor (Figure 4B, black line). Interestingly, animals show the typical endothermic increase in MR when active and exposed to low T_a (Figure 4B, red line), but at the same time can experience torpor bouts at any T_a above the critical defended temperature. Monitos cluster together in hibernacula, which permit them to limit heat loss (Figure 4C) but, when food supplemented, individuals experience frequent arousals in winter (Figure 4D). At the same time, animals can experience long torpor episodes of up to 31 days without feeding and showing collective arousals and euthermic periods of 24–48 h (Figure 4E). Thus, monitos represent a case of a species experiencing both daily torpor and hibernation, and that modulate the use of heterothermy on a day-to-day basis depending on food supply (Nespolo et al., 2020). This flexible hibernator has been one of the best studied marsupial models of heterothermy from the molecular point of view.

FIGURE 3

Figure 3. Hypothetical (but realistic) annual budget of energy and activity in monitos and mouse lemurs. Curves for South American monito del monte (Dromiciops gliroides) (left panel) are based on several descriptions of the reproductive cycle (Muñoz-Pedreros et al., 2005), seasonal variations in activity, adiposity and body mass (Celis-Diez et al., 2012; Franco et al., 2017) and food availability (Franco et al., 2011; di Virgilio et al., 2014). After reproduction in March (end of summer), monitos start to reduce activity and energy expenditure, and accumulate almost twice their body size in fat (Franco et al., 2017). The net annual energy balance is usually close to zero and varies depending on cold winter temperatures, allowing torpor to occur. Annual variations of body mass, MR (Perret, 1997) and food intake in captive male mouse lemurs (Microcebus murinus) native to Madagascar (right panel) show drastic changes across seasons, similar to that observed in wild conditions. At the beginning of winter, male mouse lemurs make extensive use of daily torpor to save energy and therefore fatten to increase their body mass by ∼40%. They reach a plateau in the middle of winter and spontaneously increase their MR while food intake is still low. Such changes coincide with the recrudescence of the reproductive axis, and lead to body mass loss. High MRs during and after mating season are supported by increased foraging, thus leading to stable body mass throughout the summer.

FIGURE 4

Figure 4. Flexible hibernation in Dromiciops gliroides. (A) a single torpor bout of 8 days in one individual; (B) predicted resting metabolic rate (RMR) in one individual followed during one winter, using the formula RMR = c(T_b–T_a); from an intraperitoneal data logger) where c = minimum thermal conductance for D. gliroides (c = 3.49 J g^–1h^–1°C^–1), from Bozinovic et al. (2004) showing the frequent torpor episodes at relatively warm temperature except when reaching the critical T_A = 5°C, when thermoregulation in torpor begins (black line); (C) social hibernation showing animals in torpor and thermographic images of the groups; (D) body temperatures in a single individual during one hibernating cycle in the field showing the frequent arousals (this animal received supplementary food) and, (E) skin temperature (red) of a cluster of five individuals that were in torpor during 3 weeks (no food available); this plot shows the last 8 days (animals remained in the same site during these normothermic episodes).

Monitos as a Model of Marsupial Torpor

Although hundreds of studies have described the physiological underpinnings of heterothermy in marsupials, especially for Australian species (summarized in Geiser and Körtner, 2010), the molecular biology and biochemistry of marsupial heterothermy has been particularly well described in monitos, a South American marsupial. Monitos trigger their heterothermic state primarily due to a drop in T_a, in food-deprived individuals with photoperiod and fat stores also being important modulators of torpor susceptibility (Nespolo et al., 2010, 2020). The transition from endothermy to heterothermy is characterized by passive cooling after a sudden reduction in MR, to a limit of about 1–2°C, when monitos start defending T_b by active thermoregulation (Figure 4B). It is highly likely that monitos hibernate at freezing temperatures, since several populations in Chile and Argentina are distributed in Andean locations where temperatures go below zero in winter (Balazote-Oliver et al., 2017; Valladares-Gómez et al., 2019). During torpor in monitos, usually T_b remains one or two degrees above T_a, and animals could spend several weeks in this condition, where MRs are of about 5% of normothermic values, heart frequency can reach 3 beats per minute and breathing frequency could be less than 1 per minute (Nespolo et al., 2010). Deep torpor is common in winter, but monitos often experience daily torpor in summer and spring (Figure 4C).

The Gray Mouse Lemur, a Primate Model of Flexible Heterothermy

The gray mouse lemur (M. murinus; Strepsirrhini; Lemuriformes; Cheirogaleidae) is a small nocturnal primate species (body mass: 60–90 g) originating from Madagascar. Half-life expectancy is around 5.7 years but animals can live for 8–10 years in captivity (Perret, 1997). The species as been widely used as a model for aging studies (Languille et al., 2012) and exhibits robust changes in body composition that are synchronized on photoperiodic cycles, a feature that is conserved in captivity (Perret and Aujard, 2001). These variations in body composition result from deep changes in physiological characteristics during winter, in particular the use of hypometabolism, even in captivity (Figure 5). Mouse lemurs adjust the depth and duration of torpor bouts according to feeding status and T_a (Figures 5A,B), and show an interesting inter-variability in their response (Figure 5C). Recent work (Terrien et al., 2017) has shown that captive male mouse lemurs acclimated to winter-like photoperiod (10 h/day) for 6 months and maintained in constant favorable housing conditions exhibit large changes in body mass explained by variations in metabolic levels and food intake. Such changes result in a biphasic phenotype: a phase of massive body mass gain followed by the spontaneous arrest of fattening. Strikingly, mouse lemurs are protected against glucose intolerance during fattening, but not during the second half of winter when they lose fat and exhibit increased fasting insulin levels. Most importantly, these natural transitions are fully reversible, and never reach pathological levels, as reflected by the apparent absence of an inflammatory response (Terrien et al., 2017). The second half of winter corresponds (only for male mouse lemurs) to the recrudescence of the reproductive system, which is completely regressed during the first half of winter (Perret and Aujard, 2001). This regression/recruitment phenotype can be interpreted as a yearly puberty that could repeatedly occur in both males and females (read below), year after year.

FIGURE 5

Figure 5. Flexibility of heterothermy in captive Microcebus murinus. (A) Captive mouse lemurs adjust the depth and duration of torpor bouts depending on food supply (control CTL vs. 30% caloric restriction CR) and ambient temperature (25 vs. 12°C); (B) Example of thermographic image of a mouse lemur acclimated to 12°C; the surface temperature is maintained very low, witnessing the insulating capacity of the animal, except at the inter-capsular region where brown adipose tissue is present; (C) Graph showing the relationship between minimal core temperature and energy expenditure during the resting phase recorded in animals acclimated to 25 and 12°C; the data clearly demonstrate the existence of an inter-individual variability in the response to cold (either maintenance of normothermia or deep torpor bouts); (D) Data showing the rise of surface temperature (heat dissipation mostly through hands and feet) following the injection of isoproterenol, a chemical that stimulates non-shivering thermogenesis.

Although generally considered to be a daily heterotherm, records of hibernation episodes (up to 30 days) have been reported in wild female gray mouse lemurs (Schmid and Ganzhorn, 2009). There seems to have a strong inter-individual variability in this feature, probably due to differences in habitat structure (habitat fragmentation), nutritional state (food availability), and body composition (fat stores). Torpor is triggered to save energy and water (Humphries et al., 2003b) but is somehow also a costly process, as highlighted by recent work on telomere dynamics during hibernation (Giroud et al., 2014; Hoelzl et al., 2016; Nowack et al., 2019). Although supported by behavioral strategies (Terrien et al., 2011), arousals from torpor are very demanding as they require the activation of NST in BAT. The activation of such process induces rapid and efficient production of body heat (Figure 5D), but also consumes fat reserves. The balance between the energy saved during torpor vs. the energetic costs of arousal is known to be altered by the aging process. Indeed, aged mouse lemurs display deficiencies in controlling the drop in T_b when exposed to low T_a (Terrien et al., 2008), thereby necessitating higher energy input for subsequent rewarming (Terrien et al., 2009). This leads to a faster exhaustion of fuel reserves, notably in BAT (Terrien et al., 2010a). These results are further supported by the enhanced use of behavioral adjustments during aging in this species (Terrien et al., 2010b) as a compensation for age-related deficiencies in physiological responses to cold exposure.

An additional parameter, quite neglected in the literature, that introduces variability in the heterothermic response is sex (Vuarin et al., 2015). Indeed, hibernation episodes during the dry season have only been reported in females but not males (Schmid and Kappeler, 1998; Schmid, 1999), supporting the “female thrifty hypothesis” that confers to females an enhanced capacity to display hypometabolism (Jonasson and Willis, 2011). Such sex bias could be linked to the phenological shift that exists between males and females in the yearly period during which they invest energy in reproduction. Female reproductive investment centers on gestation, lactation, and juvenile care and occurs during the wet favorable season. By contrast, male mouse lemur reproductive investment centers on male-to-male competition at the mating season (early summer) and, most importantly, on spermatogenesis that is a long-lasting process and has to start at the end of the dry season to ensure male readiness at the mating season. Such phenological differences between males and females might determine the flexibility of torpor bouts in mouse lemurs. There are a number of indications of enhanced torpor efficiency in females as compared to males. Indeed, although hibernation episodes have never been observed in captivity, captive female mouse lemurs are capable of using torpor during gestation and lactation when facing food shortage (Canale et al., 2012), whereas the male response to a comparable stress shows a relative inefficiency to maintain body mass by using torpor (Giroud et al., 2008; Villain et al., 2016). In addition, female mouse lemurs show enhanced mitochondrial and antioxidant capacities (Noiret et al., 2020).

Monitos and Mouse Lemurs: Daily and Seasonal Heterotherms in the Same Species

Torpor use in its depth and duration seems to be highly flexible in mouse lemurs and monitos and such flexibility probably requires specific physiological characteristics and are highly constrained by life-history traits and environmental conditions. For example, it is well referenced that hibernation capacities rely on the ability to store enough fat in order to sustain long periods of foraging and/or metabolic inactivity. As a consequence, such a phenotype is mostly expressed by larger heterotherms (Ruf and Geiser, 2015). However, among small heterotherms, including monitos and mouse lemurs, individuals showing the best body condition, i.e., the highest fat stores, also have the capacity to extend their periods of hibernation to several weeks. By contrast, inappropriate use of daily torpor or hibernation, i.e., when extrinsic (environmental) or intrinsic (body condition) factors are not favorable, might not bring the expected benefits of using hypometabolism. Indeed, a recent perspective paper proposes that physiological flexibility, including hypometabolic response to environmental constraints, not only offers ecological and physiological benefits, but also induces important costs, e.g., oxidative damage and immunodeficiency (Landes et al., 2020). Comparable observations of torpor flexibility have been made in lesser hedgehog tenrecs, Echinops telfairi (Lovegrove and Genin, 2008) and stripe-faced dunnarts (Sminthopsis macroura, Körtner and Geiser, 2009; for review see Nowack et al., 2020). Such physiological features, i.e., being able to either display short daily torpor bouts or longer weekly hibernating periods in the same species, contribute to the view that rather than being two distinct functional traits, hibernation, and daily torpor are probably extremes of a continuum (Boyles et al., 2013).

Daily Torpor and Hibernation as a Continuum of Hypometabolic Responses: Insights From Molecular Analyses

Based on observations from molecular analyses of the torpid state (for reviews, see Van Breukelen and Martin, 2002; Storey and Storey, 2004, 2007; Staples, 2011; van Breukelen and Martin, 2015), it appears that the daily expression of torpor and the successive use of multi-day or weeks-long torpor, also defined as hibernation, stand as two extremes along the continuum of hypometabolic responses. Indeed, a range of molecular experiments done with D. gliroides -a species expressing both daily torpor and seasonal hibernation- reveal that monitos experience immunological depression and muscle atrophy during torpor (Franco et al., 2013, 2017), whereas other analyses suggest the existence of tissue-specific responses regulating key molecular pathways by microRNAs to limit oxidative damage and muscle atrophy (Hadj-Moussa et al., 2016), and overexpressing cryoprotective proteins against ischemia/reperfusion stress (e.g., heat shock proteins) in the liver, heart, and brain (Nespolo et al., 2018). Interestingly, these authors found a single transcript encoding for the thioredoxin interacting protein (TXNIP) that was strongly overexpressed in several tissues, but especially in the brain. This is a potent antioxidant protein involved in regulating mitochondrial function to help suppress oxidative metabolism when oxygen is limiting and regulating a metabolic shift to anaerobic glucose catabolism by mediating inhibition of pyruvate dehydrogenase (Spindel et al., 2012; Chong et al., 2014; Yoshioka and Lee, 2014). In mice, the TXNIP gene is overexpressed in the hypothalamus, liver, and white and brown adipose during induced-torpor experiments as well as in natural torpor in Siberian hamsters, Phodopus sungorus (Hand et al., 2013; DeBalsi et al., 2014; Jastroch et al., 2016).

In the same vein, recent data show that some genes regulating daily and prolonged torpor in mouse lemurs, although altered in a much less extensive manner, are shared with that of classical hibernating species, but can also show some specificities. For example, miRNAs are known regulators of metabolic flexibility in hibernating mammals (Hadj-Moussa et al., 2016; Arfat et al., 2018; Biggar and Storey, 2018) and are particularly involved in growth processes. Such regulation of gene expression also seems to be involved in the torpor response of mouse lemurs (Biggar et al., 2018; Hadj-Moussa et al., 2020). In particular, mouse lemur torpor use involves miRNA inhibition of gene transcripts related to energetically-unfavorable cellular processes in order to facilitate metabolic suppression (Hadj-Moussa et al., 2020). For instance, small RNA sequencing of lemur miRNAs in skeletal muscle revealed that muscle-specific miR-1 and miR-133 are downregulated in mouse lemurs during daily torpor. These “myomiRs” are likely to have important roles in preventing disuse-induced muscle atrophy by targeting genes involved in regulating muscle development and energy use. For example, mir-1 and miR-133 are generally upregulated in skeletal muscles of warm-hibernating Swedish brown bears (Luu et al., 2020), the daily- or seasonally-torpid marsupial, monito del monte (Hadj-Moussa et al., 2016), and in the foot muscle of anoxia- and freeze-tolerant sea snails (Biggar et al., 2012), likely helping to protect their muscles from sustaining damage as they lie dormant. A decrease in these miRNAs in lemurs is significant and emphasizes the need to study molecular adaptations in a range of species capable of metabolic suppression, especially through the unbiased approach of sequencing all available small non-coding RNAs. Regulation of energy homeostasis through AMPK signaling, especially to promote ATP sparing and partitioning, also seems to be similar in the mouse lemur to that of classic hibernators (Zhang et al., 2015). Protein kinases involved in MAPK cascades also showed comparable regulation of expression and relative protein phosphorylation between mouse lemurs and hibernators (Biggar et al., 2015b). For instance, phosphorylation for ERK1/2 and MEK was strongly negatively regulated in skeletal muscle during torpor in the mouse lemur and in the heart of hibernating ground squirrels (Childers et al., 2019). In addition, suppression of the immune system during torpor was observed in the mouse lemur, in line with what was already known in hibernators (Franco et al., 2013; Tessier et al., 2015a). Epigenetic regulation is also involved since miR-222 was reduced during torpor in the mouse lemur as well during hibernation in ground squirrels (Wu et al., 2014). By contrast, it seems that the regulation of heat shock proteins or proteins involved in the antioxidant machinery do not change as much during daily torpor (Biggar et al., 2015a) as during hibernation (Carey et al., 1999; Morin and Storey, 2007). Furthermore, hepatic regulation of the phosphorylation state of the insulin receptor showed contrasting effects, increasing in torpid mouse lemurs (Tessier et al., 2015b), and therefore potentially reflecting an inhibition of gluconeogenesis although this process that is known to be sustained during hibernation (Green et al., 1984).

Taken together, all the data for these two small mammal models (mouse lemurs and monitos) point toward specific molecular and cellular mechanisms for the adjustment of metabolic processes and protective functions during the extreme hypometabolic down-state of torpor. These adaptive mechanisms seem to be conserved in evolution among diverse heterothermic species known to date. Taking inspiration from natural adaptations constitutes a necessity in the current world of ongoing climate change and spreading pathophysiological pandemics. Therefore, exploring these outstanding mechanisms occurring naturally in heterothermic models constitutes a unique opportunity to develop efficient responses, tools and treatments to address major environmental and health concerns. Specifically, the study of these mechanisms have potential for a better understanding of (i) protective responses against metabolic disorders such as obesity or sarcopenia (Cotton, 2016), (ii) the homeostasis of neuronal functions, e.g., the maintenance of hyper-phosphorylation of Tau proteins involved in resistance to neuro-degenerative diseases (Härtig et al., 2007; Luppi et al., 2019), and (iii) the underlying mechanisms for a state of hypothermia in humans, also called “synthetic torpor,” for therapeutic goals (Cerri, 2017) or space exploration (Choukèr et al., 2019). In the context of the latter, the study of protective mechanisms for the torpid brain is of particular interest as well as the implications for the gaseous molecule H₂S involved in the potential control and maintenance of metabolic depression and protective mechanisms in the torpid state.

Protective Mechanisms During the State of Metabolic Depression

Mechanisms to Protect the Torpid Brain

During torpor, hibernators may suppress their brain activity to the point where no electrical activity can be observed (Chatfield et al., 1951; Frerichs et al., 1994), but behind closed eyes, hibernating species coordinate the expression and activities of thousands of proteins (via changes in gene and protein expression, post-translational modification, and epigenetic controls) to ensure that one of the most important organs, the brain, is viable upon arousal (see Figure 6 for overview).

FIGURE 6

Figure 6. Overview of the protective mechanisms in the hibernating brain. Cells within the hibernating brain undergo rapidly reversible morphological changes and changes to structural proteins to prevent cellular damage. Energy expensive processes like protein translation are inhibited. Transcription factors like such as signal transduction and activators of transcription (STAT) are differentially phosphorylated but not by glycogen synthase kinase 3 beta (GSK3β) during torpor, and may regulate apoptosis via B cell lymphoma proteins (Bcl-2 and Bcl-xL) or myeloid leukemia cell differentiation protein (Mcl-1) protein expression and phosphorylation. Some microRNAs are also increased in the brain to prevent cell death. Markers of brain injury and inflammation have yet to be fully characterized, but current research shows that some cytokines that are low in the brain during torpor increase during arousal. Created with BioRender.com.

Regulation of Protein Translation and Tau Phosphorylation During Torpor

Most species suppress energetically expensive molecular processes during torpor to avoid metabolic disruption, when cellular resources of substrates to produce ATP may be limited. For example, protein translation is suppressed in brain of 13-lined ground squirrels (Ictidomys tridecemlineatus) to just 0.04% of euthermic levels (Frerichs et al., 1998) which was expected to correlate with a significant decrease in the relative expression levels of translated proteins during hibernation. However, multiple studies focusing on translational regulation in a range of hibernator tissues, including brain, indicate that hibernators use a much more rapid and reversible process to throttle translation: protein phosphorylation (Frerichs et al., 1998; Miyake et al., 2015; Logan et al., 2019). For example, ribosomal protein S6 is less phosphorylated at Ser235 during torpor, reducing its ability to bind the 5′-cap of mRNA and initiate translation (Miyake et al., 2015; Logan et al., 2019).

Consistent with these observations, hibernators including European ground squirrels (Spermophilus citellus), Arctic ground squirrels (U. parryii), black bears (Ursus americanus), and Syrian hamsters (M. auratus) use reversible phosphorylation of structural proteins such as Tau, to facilitate dendritic retraction and reduce neuronal cell signaling (Bullmann et al., 2016). Tau phosphorylation is a common feature of many tauopathies, which are neurodegenerative disorders associated with the build-up of proteinaceous plaques including tau, but fascinatingly, hibernators can reverse tau phosphorylation and clear protein aggregates upon arousal from deep torpor. Glycogen synthase kinase 3 beta (GSK3β) was postulated to regulate hibernator tau phosphorylation because it targets the specific phospho-sites that were differentially affected in hibernating Syrian hamster brain (Bullmann et al., 2016). However, new data from the Storey group indicates that GSK3β is inhibited during deep torpor in the forebrain and cerebellum of hibernating 13-lined ground squirrels (Ictidomys tridecemlineatus), based on increases in p-GSK3β (S9) during torpor (Tessier et al., 2019). As a result, it is unlikely that GSK3β is part of a conserved mechanism governing hibernator tau phosphorylation across species.

Regulation of Inflammation and Apoptosis During Hibernation

In addition to their purported importance in reducing brain activity and maintaining brain functionality during hibernation, the neurons of cold-bodied hibernators pull their dendrites back into the cell bodies that sprouted them. This appears to be an effort to reduce tissue damage to the delicate, filamentous protrusions that are responsible for accepting information from the axons of other neurons and relaying those messages to the cell body (Popov et al., 1992). Microglia (neuronal immune cells) also change their morphology during torpor in hibernating hamsters such that their protrusions are thinned and appear disconnected from the main cell body (Cogut et al., 2018; León-Espinosa et al., 2018). Microglia have important roles in regulating dendrite elongation, neuronal plasticity and synaptic transmission of signals, in addition to producing inflammatory signaling molecules. Together, changes in dendrite and microglia morphology with a decrease in core T_b could serve to inhibit neuronal activity, and possibly inflammatory signaling during deep torpor. Hibernating animals may also facilitate changes in brain structure by differentially expressing genes involved in extracellular matrix plasticity and maintenance including collagens, laminins, integrins, and matrix metalloproteinases (Schwartz et al., 2013). Matrix metalloproteinases are also differentially expressed during torpor in hibernating hamster lung (Talaei et al., 2012), suggesting a role for tissue remodeling in the survival strategies of a range of hibernating species.

Interestingly, the enzyme GSK3β may regulate the DNA binding of transcription factors that suppress neuronal apoptosis and neuroinflammation. Specifically, GSK3β activation is required for control of signal transducer and activator of transcription (STAT) 3 and STAT5 activity but is not necessary for STAT1 or STAT6 activity in astrocytes (Beurel and Jope, 2008). Neither STAT3 nor STAT5 were differentially phosphorylated during hibernation in I. tridecemlineatus forebrain, cerebellum, or brainstem, suggesting that GSK3β inhibition during torpor could correlate with a decrease in the translocation of STAT3 and STAT5 to the nucleus (Tessier et al., 2019). By contrast, compared to winter euthermic and summer euthermic controls, respectively, forebrain and brainstem STAT1 was more phosphorylated during hibernation in deep torpor. In addition, both the cerebellum and brainstem of hibernating ground squirrels exhibit increased STAT6 phosphorylation levels at Y641 during deep torpor, compared with summer active and interbout aroused animals (Tessier et al., 2019). This transcription factor may play an important role in upregulating anti-apoptotic genes such as B-cell lymphoma 2 (Bcl-2) (Lee et al., 2018). Indeed, whole brain from hibernating ground squirrels shows a significant increase in the protein and phosphorylation levels of multiple anti-apoptotic proteins including Bcl-2, Bcl-xL, Bax-inhibitor 1, and Mcl-1 (Rouble et al., 2013). These molecular responses during torpor could help hibernating ground squirrels prevent brain damage during times of nutrient limitation.

Signal transducer and activator of transcription 6 has been implicated in mediating inflammation by facilitating macrophage recruitment, mucus production, and smooth muscle contractility, suggesting that its upregulation in the hibernating ground squirrel brain may help regulate neuroinflammation during natural torpor. Hibernators including hamsters and ground squirrels can mount inflammatory responses during deep torpor (Kurtz and Carey, 2007). At the molecular level, hibernators increase the local levels of pro- and anti-inflammatory cytokines (IL-1β, IL-4, IL-6, IL-10, TNFα, interferon γ) and potentially, leukocytes, based on increases in the relative levels of cell surface markers, in order to rapidly respond to exogenous and/or endogenous damage markers as they arouse from a hibernation bout (Kurtz and Carey, 2007; Cogut et al., 2018). Endogenous markers of damage can be sensed by receptors such as the receptor for advanced glycation end products (RAGE), that can then activate MAPK and JAK/STAT signaling cascades to increase the expression of pro-inflammatory transcription factors like NFκB. RAGE expression was recently discovered to be upregulated in hibernating ground squirrel adipose tissues, particularly as they enter or exit a hibernating bout (Logan and Storey, 2018). An increase in RAGE expression during entrance into deep torpor is consistent with a suppression of mitochondrial activity and therefore a potential for less efficient electron transport through the electron transport chain leading to ROS formation. Additionally, an increase in RAGE levels in arousing animals, when ROS levels increase as a result of increased breathing and MRs and elevated mitochondrial activity, suggest that RAGE is able to sense and respond to biomarkers of oxidative stress. Consistently, BAT in hibernating arctic ground squirrels shows increased levels of lipid peroxides and protein carbonyls during the rewarming period, and these oxidized lipids and proteins may be sensed by receptors such as RAGE to trigger their removal during interbout arousal (Orr et al., 2009). In addition, NFκB signaling is upregulated during deep torpor and arousal in ground squirrel cardiac muscle, skeletal muscle, and intestine, as well as hamster lung, which could contribute to increased pro-inflammatory gene expression (Carey et al., 2000; Allan and Storey, 2012; Talaei et al., 2012; Wei et al., 2018a). Interestingly, studies show that deep hibernators injected with inflammatory stimulants only respond to the exogenous markers of cell stress (e.g., Escherichia coli lipopolysaccharide) during interbout arousals, measured as an increase in T_b relative to saline-injected ground squirrels (Prendergast et al., 2002). By contrast, forebrain shows no signs of oxidized proteins (endogenous damage markers) during deep torpor, compared with euthermic controls, suggesting that brain may use molecular mechanisms such as elevated antioxidant capacity to prevent the accumulation of damaged proteins during hibernation (Orr et al., 2009). Other damage markers such as 8-hydroxy-2′-deoxyguanosine or markers of mitochondrial dysfunction have yet to be assessed in brain cells, in conjunction with an analysis of pro-inflammatory signaling cascades. However, an analysis of CD16–CD32 and CD68 expression as markers of microglia activity revealed that hibernating hamsters do not increase microglial activity (León-Espinosa et al., 2018). Though microglia do not display advanced morphological changes associated with microglial activation, the retraction of microglial dendrites and increases in microglial cell body to cell size ratio during deep torpor, as well as increases in the relative transcript levels of pro-inflammatory IL-1β and IL-6 upon arousal point to a role for microglia in the regulation of neuronal inflammation (Cogut et al., 2018). Together, these results suggest that hibernators may induce inflammation at particular time points of the torpor-arousal cycle, but much has yet to be explored with regard to the molecular mechanisms controlling hibernator neuroinflammation.

Other Brain Protective Mechanisms in Hibernation

Neuroprotection may also be facilitated through epigenetic mechanisms, including differential microRNA expression. In a study that focused on miRNAs that are commonly dysregulated in neurodegenerative conditions, hibernating little brown bats (Myotis lucifugus) showed significant increases in microRNAs during deep torpor that were involved in regulating focal adhesion, a process that involves changes in neuronal cell cytoskeletal structure, cell cycle progression, and cell death (Biggar and Storey, 2014). For example, miR-21, -29, and -103 were all significantly increased in brain of hibernating M. lucifugus and all have important roles in decreasing apoptosis (Roshan et al., 2014; Zhou and Zhang, 2014). These data, in conjunction with protein/phosphorylation data from I. tridecemlineatus studies and gene transcript data from hibernating greater horseshoe bats (Rhinolophus ferrumequinum) (Lei et al., 2014), suggest that hibernators may use conserved regulatory mechanisms acting at multiple levels (non-coding RNA, gene, protein, and post-translational) to limit neuronal cell death during hibernation in deep torpor.

Role of H2S in Protective Mechanisms During Hibernation

Ever since the paper by Blackstone et al. (2005) that showed that inhalation of low concentrations of H₂S confers a reversible metabolic suppression in mice, people have wondered whether H₂S is involved in hibernation, for instance by inducing or maintaining torpor. Mechanistically, there are good reasons to explore this, since H₂S can profoundly reduce mitochondrial respiration through inhibition of complex IV (cytochrome c oxidase) (Khan et al., 1990). Moreover, the past 15 years of H₂S research has disclosed an array of protective effects that may be highly instrumental in hibernation to safeguard organ function and integrity in the face of hypoxia and/or excess reactive oxygen species production (Carey et al., 2000; Orr et al., 2009; Wu and Storey, 2014).

Endogenous H₂S Production and Physiological Effects

Involvement of H₂S in hibernation, be it as a metabolic suppressor or cell protective agent, requires it to be generated by the animal. In mammals, endogenous H₂S is produced by three enzymes embedded in the trans-sulfuration pathway, i.e., cystathionine β-synthase (CBS), cystathionine γ-lyase (CSE), and 3-mercaptopyruvate sulfur transferase (3MST). The trans-sulfuration pathway generates H₂S as a byproduct of methionine to cysteine conversion (reviewed in Zuhra et al., 2020), the latter amino acid being a crucial component of the antioxidant, glutathione. CBS and 3-MST are the major H₂S producing enzymes in the nervous system (Abe and Kimura, 1996; Bruintjes et al., 2014), whereas CSE is more abundant in the cardiovascular system (Yang et al., 2008). CBS and CSE are also secreted by endothelial cells and hepatocytes, and confers H₂S production in plasma (Bearden et al., 2010). Next to enzymatic synthesis, endogenous H₂S may also be derived from “sulfide” pools. Pathways are detailed in Figure 7 (left panels).

FIGURE 7

Figure 7. Endogenous production and physiology of hydrogen sulfide (H₂S). H₂S is enzymatically produced by the trans-sulfuration pathway or by alternative reactions, or derived from compounds binding sulfur (left side panels). H₂S producing enzymes differ in various ways, including their dependence on co-factors, cellular, and tissue distribution. Optimal function for both cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) is dependent on binding of pyridoxal-5′-phosphate (PLP, or vitamin B6) as a co-factor. In addition, S-adenosyl-L-methionine (SAM), the universal methyl group donor synthesized as an intermediate in the conversion of methionine to homocysteine, serves as a potent allosteric activator of CBS. The cellular localization of CBS and CSE is mainly cytosolic, yet they translocate to mitochondria under stressful conditions (Fu et al., 2012; Teng et al., 2013). 3-mercaptopyruvate sulfur transferase (3-MST) is located in both cytosol and mitochondria, with its activity increasing threefold in mitochondria (Nagahara et al., 1998). Endogenous H₂S may also be derived non-enzymatically from “bound sulfur” pools. Upper compounds are sulfane sulfurs, such as thiosulfate (S₂O₃^2–), persulfides (R-S-SH) and polysulfides (R-S_n-R), which release H₂S in the presence of reducing substances including glutathione (GSH) and cysteine (Ishigami et al., 2009) or by the action of 3-MST (Shibuya et al., 2009). Lower depicted compounds are acid-labile sulfides, which consist mainly of iron-sulfur clusters, of which the cubane-type [4Fe-4S] cluster is most common (Johnson et al., 2005). They are best known for their presence in proteins involved in oxidation-reduction reactions, including those of the electron transport chain in mitochondria. While degradation of iron-sulfur clusters releases H₂S, this occurs at strong acidic conditions (pH < 5.4), likely limiting their contribution to endogenous H₂S generation even under pathological conditions (Ishigami et al., 2009). H₂S affects mitochondria (upper right panel) and produces antioxidant defense (lower right panel). Stimulation of oxygen consumption and ATP production occurs at low H₂S concentration and originates from electron donation at coenzyme Q (CoQ) between complexes II and III (Goubern et al., 2007; Módis et al., 2013). At high concentrations, H₂S competitively and reversibly inhibits cytochrome c oxidase (complex IV) (Khan et al., 1990; Módis et al., 2014). Moreover, high concentrations of H₂S still donate electrons at coenzyme Q, which now reversely flow to complex II, reducing fumarate into succinate (Fu et al., 2012). Moreover, H₂S activates the mitochondrial K_ATP channel (Zhao et al., 2001; Lu et al., 2019) and blocks the Ca²⁺-mediated opening of the mitochondria permeability transition pore (MPTP) channel, thus supporting maintenance of mitochondrial membrane potential (Yao et al., 2010; Lu et al., 2019; Papu John et al., 2019). In addition, H₂S serves as an antioxidant and increases glutathione levels. Further, H₂S activates the prominent antioxidant master switch, nuclear factor−erythroid 2−related factor 2 (Nrf2), by sulfhydration of cysteine residues of Kelch-like ECH-associated protein 1 (Keap1) dimer. This breaks Keap1 inhibitory binding to Nrf2 and allows for Nrf2 to translocate to the nucleus (Hourihan et al., 2012; Yang et al., 2012), where it activates expression of antioxidant and cytoprotective genes, such as glutathione peroxidase (Gpx), thioredoxin (Trx), superoxide dismutase (SOD), and heme oxygenase-1 (HO-1). H₂S mediated sulfhydration of other proteins confers additional cell protective effects (Mustafa et al., 2009; Zhao et al., 2014). For example, H₂S activates the histone deacetylase sirtuin-1 (SIRT1) through its sulfhydration, leading to deacetylation of histones and condensation of chromatin, affecting an array of transcription and nuclear receptors. SIRT1 activation thus influences abundant cellular functions, including stimulation of mitochondrial metabolism and biogenesis, antioxidant pathways, DNA repair and inflammation (Du et al., 2018). ARE, antioxidant response element; Cyt C, cytochrome c, FOXO3, Forkhead box protein O3; PGC-1α, proliferator-activated receptor gamma coactivator 1-alpha. Created with BioRender.com.

Hydrogen sulfide confers a wide array of physiological effects. Firstly, it affects mitochondria in a concentration-dependent manner. In brief, at low concentrations, the prevailing effect is stimulation of oxygen consumption and ATP production, whereas high H₂S concentrations inhibit mitochondrial respiration. Secondly, H₂S confers potent antioxidant effects, both directly through scavenging of free radicals and indirectly by upregulation of various antioxidant pathways. Mechanisms of these and additional protective pathways are detailed in Figure 7 (right panels).

H₂S in Hibernation

Despite the booming number of publications on H₂S over the past decade (Szabo, 2018), there is a surprising paucity of data on the involvement of H₂S in hibernation. To date only two studies have reported on the subject. Talaei et al. (2012) reported an increase in CBS abundance with increased production of H₂S in lung tissue of torpid Syrian hamster (M. auratus), that was rapidly reversed to summer levels during interbout arousal. Increased H₂S levels were shown to quench Zn²⁺, and possibly drive torpor related lung remodeling (Talaei et al., 2011b) by lowering activity of Zn²⁺-dependent enzymes including metalloproteases and angiotensin converting enzyme (ACE). In addition, Revsbech et al. (2014) showed a change in H₂S metabolism in plasma and red blood cells of hibernating Scandinavian brown bears (Ursus arctos), with a shift toward higher levels of bound sulfane sulfur (thiosulfate and polysulfides), lower levels of free sulfide (H₂S and HS^–) and a likely increase in H₂S producing capacity in plasma and red blood cells. Furthermore, analysis of plasma metabolomics in hibernating 13-lined squirrels showed substantial regulation of substrates and products of the trans-sulfuration pathway, consistent with increased H₂S synthesis during late torpor and early arousal (D’Alessandro et al., 2017), as found in hamster lung.

The question is whether there is additional indirect evidence for a role of H₂S in hibernation. Regarding involvement of H₂S in suppression of metabolism, i.e., initiation or maintenance of torpor, there is little evidence. It should be noted that the original Blackstone et al. (2005) experiments reporting on H₂S conferring metabolic suppression in mice were performed under hypoxic conditions (17.5% of O₂). When the experiment was repeated with 21% O₂, H₂S failed to induce hypothermia (Baumgart et al., 2010; Hemelrijk et al., 2018), whereas it was successful at 17.5% O₂, albeit with a much slower onset (Hemelrijk et al., 2018). Further, exposure to severe hypoxia (5% O₂) reduced T_b close to T_a (Hemelrijk et al., 2018). Consequently, the authors attribute the observed hypothermia to hypoxemia induced vasodilation, rather than suppression of metabolism. Such a view may be supported by the absence of H₂S reduction of metabolism in other, larger animal species (Haouzi et al., 2008; Dirkes et al., 2015). As the above studies employ exogenous administration of H₂S, differences between species may also arise because of differences in pharmacokinetics and diffusion route length. Thus, a pertinent question is what the effect would be from enhancing endogenous production of H₂S. Overexpression of CBS and CSE confer longevity in Drosophila (Shaposhnikov et al., 2018), but effects on metabolism were not reported. Further, dopamine treatment strongly increases CBS expression and H₂S production in cells (Talaei et al., 2011a) and in rats in vivo (Dugbartey et al., 2015). However, neither in these experiments, nor in the literature, has a torpor-like reduction of metabolism been noted following treatment with dopamine. Finally, changes in mitochondrial function during torpor have been explored. Compared with arousal, torpor leads to a 40% lower activity of ETC complex I, a 60% lower activity of ETC complex II, but does not affect the activity of ETC complexes III, IV, or V, as measured in isolated mitochondria derived from 13-lined ground squirrel liver (Muleme et al., 2006; Brown et al., 2012; Mathers et al., 2017). Given that metabolic suppression by H₂S is through inhibition of complex IV, these data do not lend support to a prominent role of H₂S in induction or maintenance of torpor. However, a finite verdict awaits the development of (inducible) knock-out animals and/or development of selective inhibitors of H₂S producing enzymes.

On the other hand, cellular protective effects induced by H₂S may well play a role in hibernation associated antioxidant and cell protective adaptations outlined in previous sections. Notably, consistent with a prominent antioxidant effect of H₂S (Figure 7), hibernation features a strong upregulation of antioxidant mechanisms through the Nrf2 pathway (Morin et al., 2008; Ni and Storey, 2010; Wu and Storey, 2014; Yin et al., 2016; Frigault et al., 2018; Wei et al., 2018b). These and other studies report upregulation or activation of downstream genes and proteins, including, but not limited to, phosphorylated Nrf2, superoxide dismutase, glutathione reductase and peroxidase, catalase, thioredoxin, and miRNA miR-200a. Further, a number of the above studies document higher levels of glutathione in various organs, which may be compatible with enhanced flux in the trans-sulfuration pathway (D’Alessandro et al., 2017) and a coupled increase in H₂S production (Figure 7). Interestingly, hibernator cells seem to display cell-autonomous adaptations that enable upkeep of mitochondrial function during cold stress, thus safeguarding ATP production and strongly limiting oxygen radical production, as demonstrated in Syrian hamster kidney cell lines (Hendriks et al., 2017, 2020) and in neurons differentiated from pluripotent stem cells of ground squirrels (Ou et al., 2018), possibly by mild inhibition of complex I. In addition, cooled Syrian hamster kidney cells harnessed their glutathione production, thus effectively precluding ferroptotic cell death (Hendriks et al., 2020). Hibernators have been described to have developed natural resistance to chronic kidney disease (CKD), often associated with cardiovascular impairments in humans (for reviews, see Romagnani et al., 2017; Sarnak et al., 2019), despite their long hibernation fast during winter. Indeed, it was recently reported that markers of CKD differed significantly between animal species with different feeding habits and thermoregulatory behaviors, and that hibernating brown bears and garden dormice displayed higher levels of betaine and choline (known to be cardioprotective) and lower, sometimes even non-detectable, levels of trimethylamine N-oxide (TMAO), a marker of CDK in human and animals (Ebert et al., 2020). Hence, the betaine endogenously produced by the organism may protect organs (notably kidney) of bears, dormice, and other hibernators from oxidative or other damages during the hibernation period of depressed metabolism. Similarly, previous research showed that cooled hibernator kidney cells maintained production of H₂S and that dopamine induction of CBS precludes cooling-induced cell death in non-hibernator kidney cells (Talaei et al., 2011a). Together, these observations suggest that H₂S may play a role in the cell-autonomous effects observed in hibernator cells.

Taken together it appears that research efforts into the role of H₂S in hibernation need to be intensified. To date, the evidence that H₂S confers entrance or maintenance of torpor is very limited. Conversely, cell protective adaptations by H₂S have a close resemblance to those documented in hibernation, which makes H₂S involvement herein likely.

Conclusion

The flexibility of torpor use as an adaptive strategy enables different heterothermic mammals to substantially suppress their energy requirements during periods of severely reduced food availability. The phenotype of torpor is associated with marked metabolic adaptations, at the whole organism and cellular/molecular levels, that contrast with (i) the behavior of hibernation, e.g., food- vs. fat-storing hibernators, and (ii) the form of hypometabolic responses, i.e., opportunistic (daily) torpor vs. seasonal hibernation, even within one species. The torpid state is also associated with highly efficient rehabilitation and protective mechanisms ensuring continuity of proper functions in the animal, including key organs such as the brain or the heart. There are clear perspectives to be drawn out from the torpor and hibernation phenotypes, notably in terms of models for calorie restriction or intermittent fasting, e.g., fat vs. food storing species, but also for organ (e.g., brain) and tissue resistance to oxidative stress or cell protective adaptations to challenges such as ischemia-reperfusion. Given that most of these mechanisms have been addressed with placental mammals, future work comparing placentals with monotremes, marsupials and birds in a phylogenetic context would reveal convergent evolutionary pathways.

Author Contributions

All authors drafted and critically revised the manuscript.

Funding

SG was financially supported by the Austrian Science Fund (FWF, Grant[cpsenter]Nos. P27267-B25 and P31577-B25); KS and SL were funded by NSERC Canada. RH was financially supported by a grant from the European Space Agency (Research agreement collaboration 4000123556 attributed to RH). RN thanks a Fondecyt grant No. 1180917, ANID PIA/BASAL grant No. FB-0002, and Instituto Milenio de Biología Integrativa (iBio).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This review is based partially on oral presentations given during the symposium “Living at low pace: From the whole organism to the molecule” organized by SG and KS at the 10th International Congress of Comparative Physiology and Biochemistry (ICCPB) held in August 2019 in Ottawa, Canada. The authors thank Renate Hengsberger for her help with literature search and formatting of the manuscript, and the two reviewers for their helpful comments and suggestions, which greatly contributed to improve the overall quality of this review.

References

Abe, K., and Kimura, H. (1996). The possible role of hydrogen sulfide as an endogenous neuromodulator. J. Neurosci. 16, 1066–1071. doi: 10.1523/JNEUROSCI.16-03-01066.1996

PubMed Abstract | CrossRef Full Text | Google Scholar

Allan, M. E., and Storey, K. B. (2012). Expression of NF-κB and downstream antioxidant genes in skeletal muscle of hibernating ground squirrels, Spermophilus tridecemlineatus. Cell Biochem. Funct. 30, 166–174. doi: 10.1002/cbf.1832

PubMed Abstract | CrossRef Full Text | Google Scholar

Amico, G., and Aizen, M. A. (2000). Ecology. Mistletoe seed dispersal by a marsupial. Nature 408, 929–930. doi: 10.1038/35050170

PubMed Abstract | CrossRef Full Text | Google Scholar

Amico, G. C., Rodriguez-Cabal, M. A., and Aizen, M. A. (2009). The potential key seed-dispersing role of the arboreal marsupial Dromiciops gliroides. Acta Oecol. 35, 8–13. doi: 10.1016/j.actao.2008.07.003

CrossRef Full Text | Google Scholar

Arfat, Y., Chang, H., and Gao, Y. (2018). Stress-responsive microRNAs are involved in re-programming of metabolic functions in hibernators. J. Cell. Physiol. 233, 2695–2704. doi: 10.1002/jcp.26034

PubMed Abstract | CrossRef Full Text | Google Scholar

Auld, J. R., Agrawal, A. A., and Relyea, R. A. (2010). Re-evaluating the costs and limits of adaptive phenotypic plasticity. Proc. R. Soc. B 277, 503–511. doi: 10.1098/rspb.2009.1355

PubMed Abstract | CrossRef Full Text | Google Scholar

Baggio, L. L., and Drucker, D. J. (2007). Biology of Incretins: GLP-1 and GIP. Gastroenterology 132, 2131–2157. doi: 10.1053/j.gastro.2007.03.054

PubMed Abstract | CrossRef Full Text | Google Scholar

Balazote-Oliver, A., Amico, G. C., Rivarola, M. D., and Morales, J. M. (2017). Population dynamics of Dromiciops gliroides (Microbiotheriidae) in an austral temperate forest. J. Mammal. 98, 1179–1184. doi: 10.1093/jmammal/gyx051

CrossRef Full Text | Google Scholar

Bartels, W., Law, B. S., and Geiser, F. (1998). Daily torpor and energetics in a tropical mammal, the northern blossom-bat Macroglossus minimus (Megachiroptera). J. Comp. Physiol. B 168, 233–239. doi: 10.1007/s003600050141

PubMed Abstract | CrossRef Full Text | Google Scholar

Bauman, W. A., Meryn, S., and Florant, G. L. (1987). Pancreatic hormones in the nonhibernating and hibernating golden- mantled ground squirrel. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 86, 241–244. doi: 10.1016/0300-9629(87)90324-0

CrossRef Full Text | Google Scholar

Baumgart, K., Wagner, F., Gröger, M., Weber, S., Barth, E., Vogt, J. A., et al. (2010). Cardiac and metabolic effects of hypothermia and inhaled hydrogen sulfide in anesthetized and ventilated mice. Crit. Care Med. 38, 588–595. doi: 10.1097/CCM.0b013e3181b9ed2e

PubMed Abstract | CrossRef Full Text | Google Scholar

Bearden, S. E., Beard, R. S. Jr., and Pfau, J. C. (2010). Extracellular transsulfuration generates hydrogen sulfide from homocysteine and protects endothelium from redox stress. Am. J. Physiol. Heart Circ. Physiol. 299, H1568–H1576. doi: 10.1152/ajpheart.00555.2010

PubMed Abstract | CrossRef Full Text | Google Scholar

Beurel, E., and Jope, R. S. (2008). Differential regulation of STAT family members by glycogen synthase kinase-3. J. Biol. Chem. 283, 21934–21944. doi: 10.1074/jbc.M802481200

PubMed Abstract | CrossRef Full Text | Google Scholar

Bieber, C., Turbill, C., and Ruf, T. (2018). Effects of aging on timing of hibernation and reproduction. Sci. Rep. 8:13881. doi: 10.1038/s41598-018-32311-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., Kornfeld, S. F., Maistrovski, Y., and Storey, K. B. (2012). MicroRNA regulation in extreme environments: differential expression of MicroRNAs in the intertidal snail Littorina littorea during extended periods of freezing and anoxia. Genomics Proteomics Bioinformatics 10, 302–309. doi: 10.1016/j.gpb.2012.09.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., Luu, B. E., Wu, C. W., Pifferi, F., Perret, M., and Storey, K. B. (2018). Identification of novel and conserved microRNA and their expression in the gray mouse lemur. Microcebus murinus, a primate capable of daily torpor. Gene 677, 332–339. doi: 10.1016/j.gene.2018.08.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., and Storey, K. B. (2014). Identification and expression of microRNA in the brain of hibernating bats. Myotis lucifugus. Gene 544, 67–74. doi: 10.1016/j.gene.2014.04.048

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., and Storey, K. B. (2018). Functional impact of microRNA regulation in models of extreme stress adaptation. J. Mol Cell Biol 10, 93–101. doi: 10.1093/jmcb/mjx053

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., Wu, C. W., Tessier, S. N., Zhang, J., Pifferi, F., Perret, M., et al. (2015a). Modulation of gene expression in key survival pathways during daily torpor in the gray mouse lemur. Microcebus murinus. Genomics Proteomics Bioinformatics 13, 111–118. doi: 10.1016/j.gpb.2015.03.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Biggar, K. K., Wu, C. W., Tessier, S. N., Zhang, J., Pifferi, F., Perret, M., et al. (2015b). Primate torpor: regulation of stress-activated protein kinases during daily torpor in the gray mouse lemur. Microcebus murinus. Genomics Proteomics Bioinformatics 13, 81–90. doi: 10.1016/j.gpb.2015.03.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Blackstone, E., Morrison, M., and Roth, M. B. (2005). H2S induces a suspended animation-like state in mice. Science 308, 518. doi: 10.1126/science.1108581

PubMed Abstract | CrossRef Full Text | Google Scholar

Boag, D. A., and Murie, J. O. (1981). Weight in relation to sex, age, and season in Columbian ground squirrels (Sciuridae. Rodentia). Can. J. Zool. 59, 999–1004. doi: 10.1139/z81-139

CrossRef Full Text | Google Scholar

Bouma, H. R., Verhaag, E. M., Otis, J. P., Heldmaier, G., Swoap, S. J., Strijkstra, A. M., et al. (2012). Induction of torpor: mimicking natural metabolic suppression for biomedical applications. J. Cell. Physiol. 227, 1285–1290. doi: 10.1002/jcp.22850

PubMed Abstract | CrossRef Full Text | Google Scholar

Boyer, B. B., Ormseth, O. A., Buck, L., Nicolson, M., Pelleymounter, M. A., and Barnes, B. M. (1997). Leptin prevents posthibernation weight gain but does not reduce energy expenditure in arctic ground squirrels. Comp. Biochem. Physiol. C 118, 405–412. doi: 10.1016/S0742-8413(97)00172-2

CrossRef Full Text | Google Scholar

Boyles, J. G., Dunbar, M. B., Storm, J. J., and Brack, V. Jr. (2007). Energy availability influences microclimate selection of hibernating bats. J. Exp. Biol. 210(Pt 24), 4345–4350. doi: 10.1242/jeb.007294

PubMed Abstract | CrossRef Full Text | Google Scholar

Boyles, J. G., Thompson, A. B., McKechnie, A. E., Malan, E., Humphries, M. M., and Careau, V. (2013). A global heterothermic continuum in mammals. Global Ecol. Biogeogr. 22, 1029–1039. doi: 10.1111/Geb.12077

CrossRef Full Text | Google Scholar

Bozinovic, F., Contreras, L., Rosenmann, M., and Torres-Mura, J. C. (1985). Bioenergética de Myotis chiloensis (Quiroptera: Vespertilionidae). Rev. Chil. Hist. Nat. 58, 39–45.

Google Scholar

Bozinovic, F., and Marquet, P. A. (1991). Energetics and torpor in the Atacama desert-dwelling rodent Phyllotis darwini rupestris. J. Mammal. 72, 734–738. doi: 10.2307/1381835

CrossRef Full Text | Google Scholar

Bozinovic, F., Muñoz, J. L. P., Naya, D. E., and Cruz-Neto, A. P. (2007). Adjusting energy expenditures to energy supply: food availability regulates torpor use and organ size in the Chilean mouse-opossum Thylamys elegans. J. Comp. Physiol. B 177, 393–400. doi: 10.1007/s00360-006-0137-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Bozinovic, F., Ruiz, G., and Rosenmann, M. (2004). Energetics and torpor of a South American “living fossil”, the microbiotheriid Dromiciops gliroides. J. Comp. Physiol. B 174, 293–297. doi: 10.1007/s00360-004-0414-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Brown, J. C. L., Chung, D. J., Belgrave, K. R., and Staples, J. F. (2012). Mitochondrial metabolic suppression and reactive oxygen species production in liver and skeletal muscle of hibernating thirteen-lined ground squirrels. Am. J. Physiol. Reg. Int. Comp. Physiol. 302, R15–R28. doi: 10.1152/ajpregu.00230.2011

PubMed Abstract | CrossRef Full Text | Google Scholar

Bruintjes, J. J., Henning, R. H., Douwenga, W., and van der Zee, E. A. (2014). Hippocampal cystathionine beta synthase in young and aged mice. Neurosci. Lett. 563, 135–139. doi: 10.1016/j.neulet.2014.01.049

PubMed Abstract | CrossRef Full Text | Google Scholar

Bullmann, T., Seeger, G., Stieler, J., Hanics, J., Reimann, K., Kretzschmann, T. P., et al. (2016). Tau phosphorylation-associated spine regression does not impair hippocampal-dependent memory in hibernating golden hamsters. Hippocampus 26, 301–318. doi: 10.1002/hipo.22522

PubMed Abstract | CrossRef Full Text | Google Scholar

Canale, C. I., Perret, M., and Henry, P. Y. (2012). Torpor use during gestation and lactation in a primate. Naturwissenschaften 99, 159–163. doi: 10.1007/s00114-011-0872-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Careau, V., Bininda-Emonds, O. R. P., Thomas, D. W., Reale, D., and Humphries, M. M. (2009). Exploration strategies map along fast-slow metabolic and life-history continua in muroid rodents. Funct. Ecol. 23, 150–156. doi: 10.1111/j.1365-2435.2008.01468.x

CrossRef Full Text | Google Scholar

Carey, H. V. (1990). Seasonal changes in mucosal structure and function in ground squirrel intestine. Am. J. Physiol. Reg. Int. Comp. Physiol. 259, R385–R392. doi: 10.1152/ajpregu.1990.259.2.R385

PubMed Abstract | CrossRef Full Text | Google Scholar

Carey, H. V. (1995). Gut feelings about hibernation. Physiology 10, 55–61. doi: 10.1152/physiologyonline.1995.10.2.55

CrossRef Full Text | Google Scholar

Carey, H. V., Andrews, M. T., and Martin, S. L. (2003). Mammalian hibernation: cellular and molecular responses to depressed metabolism and low temperature. Physiol. Rev. 83, 1153–1181. doi: 10.1152/physrev.00008.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

Carey, H. V., Frank, C. L., and Seifert, J. P. (2000). Hibernation induces oxidative stress and activation of NF-κB in ground squirrel intestine. J. Comp. Physiol. B 170, 551–559. doi: 10.1007/s003600000135

PubMed Abstract | CrossRef Full Text | Google Scholar

Carey, H. V., and Martin, S. L. (1996). Preservation of intestinal gene expression during hibernation. Am. J. Physiol. Gastro. Liver Physiol. 271, G804–G813. doi: 10.1152/ajpgi.1996.271.5.G805

PubMed Abstract | CrossRef Full Text | Google Scholar

Carey, H. V., Sills, N. S., and Gorham, D. A. (1999). Stress proteins in mammalian hibernation. Am. Zool. 39, 825–835. doi: 10.1093/icb/39.6.825

PubMed Abstract | CrossRef Full Text | Google Scholar

Carpenter, F. L. (1974). Torpor in an andean hummingbird: its ecological significance. Science 183, 545–547. doi: 10.1126/science.183.4124.545

PubMed Abstract | CrossRef Full Text | Google Scholar

Castex, C., Tahri, A., Hoo-Paris, R., and Sutter, B. C. J. (1984). Insulin secretion in the hibernating edible dormouse (Glis glis): in vivo and in vitro studies. Comp. Biochem. Physiol. A 79, 179–183. doi: 10.1016/0300-9629(84)90729-1

CrossRef Full Text | Google Scholar

Celis-Diez, J. L., Hetz, J., Marín-Vial, P. A., Fuster, G., Necochea, P., Vásquez, R. A., et al. (2012). Population abundance, natural history, and habitat use by the arboreal marsupial Dromiciops gliroides in rural Chiloé Island. Chile. J. Mammal. 93, 134–148. doi: 10.1644/10-MAMM-A-406.1

CrossRef Full Text | Google Scholar

Cerri, M. (2017). The central control of energy expenditure: exploiting torpor for medical applications. Annu. Rev. Physiol. 79, 167–186. doi: 10.1146/annurev-physiol-022516-034133

PubMed Abstract | CrossRef Full Text | Google Scholar

Chanon, S., Chazarin, B., Toubhans, B., Durand, C., Chery, I., Robert, M., et al. (2018). Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells. Sci. Rep. 8:5525. doi: 10.1038/s41598-018-23891-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Chatfield, P. O., Lyman, C. P., and Purpura, D. P. (1951). The effects of temperature on the spontaneous and induced electrical activity in the cerebral cortex of the golden hamster. Electroencephalogr. Clin. Neurophysiol. 3, 225–230. doi: 10.1016/0013-4694(51)90015-6

CrossRef Full Text | Google Scholar

Childers, C. L., Tessier, S. N., and Storey, K. B. (2019). The heart of a hibernator: EGFR and MAPK signaling in cardiac muscle during the hibernation of thirteen-lined ground squirrels. Ictidomys tridecemlineatus. PeerJ 7:e7587. doi: 10.7717/peerj.7587

PubMed Abstract | CrossRef Full Text | Google Scholar

Chong, C. R., Chan, W. P. A., Nguyen, T. H., Liu, S. F., Procter, N. E. K., Ngo, D. T., et al. (2014). Thioredoxin-interacting protein: pathophysiology and emerging pharmacotherapeutics in cardiovascular disease and diabetes. Cardiovasc. Drugs Ther. 28, 347–360. doi: 10.1007/s10557-014-6538-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Choukèr, A., Bereiter-Hahn, J., Singer, D., and Heldmaier, G. (2019). Hibernating astronauts-science or fiction? Pflugers Arch. Eur. J. Physiol. 471, 819–828. doi: 10.1007/s00424-018-2244-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Clarke, I. J., and Caraty, A. (2013). Kisspeptin and seasonality of reproduction. Adv. Exp. Med. Biol. 784, 411–430. doi: 10.1007/978-1-4614-6199-9_19

CrossRef Full Text | Google Scholar

Cogut, V., Bruintjes, J. J., Eggen, B. J. L., van der Zee, E. A., and Henning, R. H. (2018). Brain inflammatory cytokines and microglia morphology changes throughout hibernation phases in Syrian hamster. Brain Behav Immun. 68, 17–22. doi: 10.1016/j.bbi.2017.10.009

PubMed Abstract | CrossRef Full Text | Google Scholar

Concannon, P., Levac, K., Rawson, R., Tennant, B., and Bensadoun, A. (2001). Seasonal changes in serum leptin, food intake, and body weight in photoentrained woodchucks. Am. J. Physiol. Reg. Int. Comp. Physiol. 281, R951–R959. doi: 10.1152/ajpregu.2001.281.3.R951

PubMed Abstract | CrossRef Full Text | Google Scholar

Cotton, C. J. (2016). Skeletal muscle mass and composition during mammalian hibernation. J. Exp. Biol. 219, 226–234. doi: 10.1242/jeb.125401

PubMed Abstract | CrossRef Full Text | Google Scholar

Cummings, D. E. (2006). Ghrelin and the short- and long-term regulation of appetite and body weight. Physiol. Behav. 89, 71–84. doi: 10.1016/j.physbeh.2006.05.022

PubMed Abstract | CrossRef Full Text | Google Scholar

D’Alessandro, A., Nemkov, T., Bogren, L. K., Martin, S. L., and Hansen, K. C. (2017). Comfortably numb and back: plasma metabolomics reveals biochemical adaptations in the hibernating 13-lined ground squirrel. J. Proteome Res. 16, 958–969. doi: 10.1021/acs.jproteome.6b00884

PubMed Abstract | CrossRef Full Text | Google Scholar

Dardente, H., Wood, S., Ebling, F., and Sáenz de Miera, C. (2019). An integrative view of mammalian seasonal neuroendocrinology. J. Neuroendocrinol 31:e12729. doi: 10.1111/jne.12729

PubMed Abstract | CrossRef Full Text | Google Scholar

Dark, J. (2005). Annual lipid cycles in hibernators: integration of physiology and behavior. Annu. Rev. Nutr. 25, 469–497. doi: 10.1146/annurev.nutr.25.050304.092514

PubMed Abstract | CrossRef Full Text | Google Scholar

Davis, D. E. (1976). Hibernation and circannual rhythms of food consumption in marmots and ground squirrels. Q. Rev. Biol. 51, 477–514. doi: 10.1086/409594

PubMed Abstract | CrossRef Full Text | Google Scholar

de Cabo, R., and Mattson, M. P. (2019). Effects of intermittent fasting on health. Aging, and Disease. New Engl. J. Med. 381, 2541–2551. doi: 10.1056/NEJMra1905136

PubMed Abstract | CrossRef Full Text | Google Scholar

DeBalsi, K. L., Wong, K. E., Koves, T. R., Slentz, D. H., Seiler, S. E., Wittmann, A. H., et al. (2014). Targeted metabolomics connects thioredoxin-interacting protein (TXNIP) to mitochondrial fuel selection and regulation of specific oxidoreductase enzymes in skeletal muscle. J. Biol. Chem. 289, 8106–8120. doi: 10.1074/jbc.M113.511535

PubMed Abstract | CrossRef Full Text | Google Scholar

di Virgilio, A., Amico, G. C., and Morales, J. M. (2014). Behavioral traits of the arboreal marsupial Dromiciops gliroides during Tristerix corymbosus fruiting season. J. Mammal. 95, 1189–1198. doi: 10.1644/13-MAMM-A-281

CrossRef Full Text | Google Scholar

Dirkes, M. C., Milstein, D. M. J., Heger, M., and van Gulik, T. M. (2015). Absence of hydrogen sulfide-induced hypometabolism in pigs: a mechanistic explanation in relation to small nonhibernating mammals. Eur. Surg. Res. 54, 178–191. doi: 10.1159/000369795

PubMed Abstract | CrossRef Full Text | Google Scholar

Du, C., Lin, X., Xu, W., Zheng, F., Cai, J., Yang, J., et al. (2018). Sulfhydrated Sirtuin-1 increasing its deacetylation activity is an essential epigenetics mechanism of anti-atherogenesis by hydrogen sulfide. Antioxid. Redox. Signal. 30, 184–197. doi: 10.1089/ars.2017.7195

PubMed Abstract | CrossRef Full Text | Google Scholar

Dugbartey, G. J., Talaei, F., Houwertjes, M. C., Goris, M., Epema, A. H., Bouma, H. R., et al. (2015). Dopamine treatment attenuates acute kidney injury in a rat model of deep hypothermia and rewarming – The role of renal H2S-producing enzymes. Eur. J. Pharmacol. 769, 225–233. doi: 10.1016/j.ejphar.2015.11.022

PubMed Abstract | CrossRef Full Text | Google Scholar

Ebert, T., Painer, J., Bergman, P., Qureshi, A. R., Giroud, S., Stalder, G., et al. (2020). Insights in the regulation of trimetylamine N-oxide production using a comparative biomimetic approach suggest a metabolic switch in hibernating bears. Sci. Rep. 10:20323. doi: 10.1038/s41598-020-76346-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Faherty, S. L., Villanueva-Cañas, J. L., Klopfer, P. H., Albà, M. M., and Yoder, A. D. (2016). Gene expression profiling in the hibernating primate. Cheirogaleus Medius. Genome Biol. Evol. 8, 2413–2426. doi: 10.1093/gbe/evw163

PubMed Abstract | CrossRef Full Text | Google Scholar

Florant, G. L., Hoo-Paris, R., Castex, C., Bauman, W. A., and Sutter, B. C. (1986). Pancreatic A and B cell stimulation in euthermic and hibernating marmots (Marmota flaviventris): effects of glucose and arginine administration. J. Comp. Physiol. B 156, 309–314. doi: 10.1007/BF01101092

PubMed Abstract | CrossRef Full Text | Google Scholar

Florant, G. L., Porst, H., Pfeiffer, A., Hudachek, S. F., Pittman, C., Summers, S. A., et al. (2004). Fat-cell mass, serum leptin and adiponectin changes during weight gain and loss in yellow-bellied marmots (Marmota flaviventris). J. Comp. Physiol. B 174, 633–639. doi: 10.1007/s00360-004-0454-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Franco, M., Contreras, C., and Nespolo, R. F. (2013). Profound changes in blood parameters during torpor in a South American marsupial. Comp. Biochem. Physiol. A 166, 338–342. doi: 10.1016/j.cbpa.2013.07.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Franco, M., Contreras, C., Place, N. J., Bozinovic, F., and Nespolo, R. F. (2017). Leptin levels, seasonality and thermal acclimation in the Microbiotherid marsupial Dromiciops gliroides: does photoperiod play a role? Comp. Biochem. Physiol. A 203, 233–240. doi: 10.1016/j.cbpa.2016.09.025

PubMed Abstract | CrossRef Full Text | Google Scholar

Franco, M., Quijano, A., and Soto-Gamboa, M. (2011). Communal nesting, activity patterns, and population characteristics in the near-threatened monito del monte. Dromiciops gliroides. J. Mammal. 92, 994–1004. doi: 10.1644/10-MAMM-A-256.1

CrossRef Full Text | Google Scholar

Freeman, D. A., Lewis, D. A., Kauffman, A. S., Blum, R. M., and Dark, J. (2004). Reduced leptin concentrations are permissive for display of torpor in Siberian hamsters. Am. J. Physiol. Reg. Int. Comp. Physiol. 287, R97–R103. doi: 10.1152/ajpregu.00716.2003

PubMed Abstract | CrossRef Full Text | Google Scholar

French, A. R. (1988). The patterns of mammalian hibernation. Am. Sci. 76, 569–575.

Google Scholar

French, A. R. (1990). Age-class differences in the pattern of hibernation in yellow-bellied marmots. Marmota flaviventris. Oecologia 82, 93–96. doi: 10.1007/BF00318538

PubMed Abstract | CrossRef Full Text | Google Scholar

Frerichs, K. U., Kennedy, C., Sokoloff, L., and Hallenbeck, J. M. (1994). local cerebral blood flow during hibernation, a model of natural tolerance to “Cerebral Ischemia”. J. Cereb. Blood Flow Metab. 14, 193–205. doi: 10.1038/jcbfm.1994.26

PubMed Abstract | CrossRef Full Text | Google Scholar

Frerichs, K. U., Smith, C. B., Brenner, M., DeGracia, D. J., Krause, G. S., Marrone, L., et al. (1998). Suppression of protein synthesis in brain during hibernation involves inhibition of protein initiation and elongation. Proc. Natl. Acad. Sci. U.S.A. 95, 14511–14516. doi: 10.1073/pnas.95.24.14511

PubMed Abstract | CrossRef Full Text | Google Scholar

Frigault, J. J., Gaudet, J. D., and Morin, P. Jr. (2018). Investigating Nrf2-associated non-coding RNAs in the hibernating ground squirrel, Ictidomys tridecemlineatus. J. Therm. Biol. 75, 38–44. doi: 10.1016/j.jtherbio.2018.05.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Fruebis, J., Tsao, T. S., Javorschi, S., Ebbets-Reed, D., Erickson, M. R., Yen, F. T., et al. (2001). Proteolytic cleavage product of 30-kDa adipocyte complement-related protein increases fatty acid oxidation in muscle and causes weight loss in mice. Proc. Natl. Acad. Sci. U.S.A. 98, 2005–2010. doi: 10.1073/pnas.041591798

PubMed Abstract | CrossRef Full Text | Google Scholar

Fu, M., Zhang, W., Wu, L., Yang, G., Li, H., and Wang, R. (2012). Hydrogen sulfide (H2S) metabolism in mitochondria and its regulatory role in energy production. Proc. Natl. Acad. Sci. U.S.A. 109, 2943–2948. doi: 10.1073/pnas.1115634109

PubMed Abstract | CrossRef Full Text | Google Scholar

Galluser, M., Raul, F., and Canguilhem, B. (1988). Adaptation of intestinal enzymes to seasonal and dietary changes in a hibernator: the European hamster (Cricetus cricetus). J. Comp. Physiol. B 158, 143–149. doi: 10.1007/bf01075827

PubMed Abstract | CrossRef Full Text | Google Scholar

Gaudry, M. J., and Campbell, K. L. (2017). Evolution of UCP1 transcriptional regulatory elements across the mammalian phylogeny. Front. Physiol. 8:670. doi: 10.3389/fphys.2017.00670

PubMed Abstract | CrossRef Full Text | Google Scholar

Geiser, F. (2013). Hibernation. Curr. Biol. 23, R188–R193. doi: 10.1016/j.cub.2013.01.062

PubMed Abstract | CrossRef Full Text | Google Scholar

Geiser, F. (2020). Seasonal expression of avian and mammalian daily torpor and hibernation: not a simple summer-winter affair. Front. Physiol. 11:436. doi: 10.3389/fphys.2020.00436

CrossRef Full Text | Google Scholar

Geiser, F., and Körtner, G. (2010). Hibernation and daily torpor in Australian mammals. Aust. Zool. 35, 204–215. doi: 10.7882/AZ.2010.009

CrossRef Full Text | Google Scholar

Geiser, F., and Martin, G. M. (2013). Torpor in the Patagonian opossum (Lestodelphys halli): implications for the evolution of daily torpor and hibernation. Naturwissenschaften 100, 975–981. doi: 10.1007/s00114-013-1098-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Giroud, S., Blanc, S., Aujard, F., Bertrand, F., Gilbert, C., and Perret, M. (2008). Chronic food shortage and seasonal modulations of daily torpor and locomotor activity in the grey mouse lemur (Microcebus murinus). Am. J. Physiol. Reg. Int. Comp. Physiol. 294, R1958–R1967. doi: 10.1152/ajpregu.00794.2007

PubMed Abstract | CrossRef Full Text | Google Scholar

Giroud, S., Stalder, G., Gerritsmann, H., Kübber-Heiss, A., Kwak, J., Arnold, W., et al. (2018). Dietary lipids affect the onset of hibernation in the garden dormouse (Eliomys quercinus): implications for cardiac function. Front. Physiol. 9:1235. doi: 10.3389/fphys.2018.01235

PubMed Abstract | CrossRef Full Text | Google Scholar

Giroud, S., Zahn, S., Criscuolo, F., Chery, I., Blanc, S., Turbill, C., et al. (2014). Late-born intermittently fasted juvenile garden dormice use torpor to grow and fatten prior to hibernation: consequences for ageing processes. Proc. R. Soc. B 281:20141131. doi: 10.1098/rspb.2014.1131

PubMed Abstract | CrossRef Full Text | Google Scholar

Godoy-Güinao, J., Díaz, I. A., and Celis-Diez, J. L. (2018). Confirmation of arboreal habits in Dromiciops gliroides: a key role in chilean temperate rainforests. Ecosphere 9:e02424. doi: 10.1002/ecs2.2424

CrossRef Full Text | Google Scholar

Golbidi, S., Daiber, A., Korac, B., Li, H., Essop, M. F., and Laher, I. (2017). Health benefits of fasting and caloric restriction. Curr. Diab. Rep. 17:123. doi: 10.1007/s11892-017-0951-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Goubern, M., Andriamihaja, M., Nübel, T., Blachier, F., and Bouillaud, F. (2007). Sulfide, the first inorganic substrate for human cells. FASEB J. 21, 1699–1706. doi: 10.1096/fj.06-7407com

PubMed Abstract | CrossRef Full Text | Google Scholar

Green, C. J., Brosman, J. T., Fuller, B. J., Lowry, M., Stubbs, M., and Ross, B. C. (1984). Effect of hibernation on liver and kidney metabolism in 13-lined ground squirrels. Comp. Biochem. Phys. B 79, 167–171. doi: 10.1016/0305-0491(84)90009-9

CrossRef Full Text | Google Scholar

Hadj-Moussa, H., Moggridge, J. A., Luu, B. E., Quintero-Galvis, J. F., Gaitán-Espitia, J. D., Nespolo, R. F., et al. (2016). The hibernating South American marsupial, Dromiciops gliroides, displays torpor-sensitive microRNA expression patterns. Sci. Rep. 6:24627. doi: 10.1038/srep24627

PubMed Abstract | CrossRef Full Text | Google Scholar

Hadj-Moussa, H., Zhang, J., Pifferi, F., Perret, M., and Storey, K. B. (2020). Profiling torpor-responsive microRNAs in muscles of the hibernating primate Microcebus murinus. BBA Gene Struct. Exp. 1863:194473. doi: 10.1016/j.bbagrm.2019.194473

PubMed Abstract | CrossRef Full Text | Google Scholar

Hampton, M., Melvin, R. G., and Andrews, M. T. (2013). Transcriptomic analysis of brown adipose tissue across the physiological extremes of natural hibernation. PLoS One 8:e85157. doi: 10.1371/journal.pone.0085157

PubMed Abstract | CrossRef Full Text | Google Scholar

Hampton, M., Melvin, R. G., Kendall, A. H., Kirkpatrick, B. R., Peterson, N., and Andrews, M. T. (2011). Deep sequencing the transcriptome reveals seasonal adaptive mechanisms in a hibernating mammal. PLoS One 6:e27021. doi: 10.1371/journal.pone.0027021

PubMed Abstract | CrossRef Full Text | Google Scholar

Hand, L. E., Saer, B. R. C., Hui, S. T., Jinnah, H. A., Steinlechner, S., Loudon, A. S. I., et al. (2013). Induction of the metabolic regulator Txnip in fasting-induced and natural torpor. Endocrinology 154, 2081–2091. doi: 10.1210/En.2012-2051

PubMed Abstract | CrossRef Full Text | Google Scholar

Haouzi, P., Notet, V., Chenuel, B., Chalon, B., Sponne, I., Ogier, V., et al. (2008). H2S induced hypometabolism in mice is missing in sedated sheep. Resp. Physiol. Neurobiol. 160, 109–115. doi: 10.1016/j.resp.2007.09.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Härtig, W., Stieler, J., Boerema, A. S., Wolf, J., Schmidt, U., Weißfuß, J., et al. (2007). Hibernation model of tau phosphorylation in hamsters: selective vulnerability of cholinergic basal forebrain neurons – implications for Alzheimer’s disease. Eur. J. Neurosci. 25, 69–80. doi: 10.1111/j.1460-9568.2006.05250.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Healy, J. E., Ostrom, C. E., Wilkerson, G. K., and Florant, G. L. (2010). Plasma ghrelin concentrations change with physiological state in a sciurid hibernator (Spermophilus lateralis). Gen. Comp. Endocrinol. 166, 372–378. doi: 10.1016/j.ygcen.2009.12.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Healy, J. E., Richter, M. M., Suu, L., Fried, S. K., and Florant, G. L. (2008). “Changes in serum leptin concentrations with fat mass in golden-mantled ground squirrels (Spermophilus lateralis),” in Hypometabolism in Animals: Hibernation, Torpor and Cryobiology, eds B. G. Lovegrove and A. E. McKechnie (Pietermaritzburg: University of KwaZulu-Natal).

Google Scholar

Heldmaier, G., Ortmann, S., and Elvert, R. (2004). Natural hypometabolism during hibernation and daily torpor in mammals. Resp. Physiol. Neurobiol. 141, 317–329. doi: 10.1016/j.resp.2004.03.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Hemelrijk, S. D., Dirkes, M. C., van Velzen, M. H. N., Bezemer, R., van Gulik, T. M., and Heger, M. (2018). Exogenous hydrogen sulfide gas does not induce hypothermia in normoxic mice. Sci. Rep. 8:3855. doi: 10.1038/s41598-018-21729-8

PubMed Abstract | CrossRef Full Text | Google Scholar

Hendriks, K. D. W., Joschko, C. P., Hoogstra-Berends, F., Heegsma, J., Faber, K.-N., and Henning, R. H. (2020). Hibernator-derived cells show superior protection and survival in hypothermia compared to non-hibernator cells. Int. J. Mol. Sci. 21:1864. doi: 10.3390/ijms21051864

CrossRef Full Text | Google Scholar

Hendriks, K. D. W., Lupi, E., Hardenberg, M. C., Hoogstra-Berends, F., Deelman, L. E., and Henning, R. H. (2017). Differences in mitochondrial function and morphology during cooling and rewarming between hibernator and non-hibernator derived kidney epithelial cells. Sci. Rep. 7:15482. doi: 10.1038/s41598-017-15606-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoelzl, F., Cornils, J. S., Smith, S., Moodley, Y., and Ruf, T. (2016). Telomere dynamics in free-living edible dormice (Glis glis): the impact of hibernation and food supply. J. Exp. Biol. 219(Pt 16), 2469–2474. doi: 10.1242/jeb.140871

PubMed Abstract | CrossRef Full Text | Google Scholar

Hoo-Paris, R., Castex, C., Hamsany, M., Thari, A., and Sutter, B. (1985). Glucagon secretion in the hibernating edible dormouse (Glis glis). Comp. Biochem. Physiol. A 81, 277–281. doi: 10.1016/0300-9629(85)90135-5

CrossRef Full Text | Google Scholar

Hoo-Paris, R., Hamsany, M., Sutter, B. C. J., Assan, R., and Boillot, J. (1982). Plasma glucose and glucagon concentrations in the hibernating hedgehog. Gen. Comp. Endocrinol. 46, 246–254. doi: 10.1016/0016-6480(82)90206-4

CrossRef Full Text | Google Scholar

Hoo-Paris, R., and Sutter, B. C. J. (1980). Blood glucose control by insulin in the lethargic and arousing hedgehog (Erinaceus europaeus). Comp. Biochem. Physiol. A Physiol. 66, 141–143. doi: 10.1016/0300-9629(80)90371-0

CrossRef Full Text | Google Scholar

Hourihan, J. M., Kenna, J. G., and Hayes, J. D. (2012). The gasotransmitter hydrogen sulfide induces Nrf2-target genes by inactivating the Keap1 ubiquitin ligase substrate adaptor through formation of a disulfide bond between Cys-226 and Cys-613. Antioxid. Redox. Signal. 19, 465–481. doi: 10.1089/ars.2012.4944

PubMed Abstract | CrossRef Full Text | Google Scholar

Hrvatin, S., Sun, S., Wilcox, O. F., Yao, H., Lavin-Peter, A. J., Cicconet, M., et al. (2020). Neurons that regulate mouse torpor. Nature 583, 115–121. doi: 10.1038/s41586-020-2387-5

PubMed Abstract | CrossRef Full Text | Google Scholar

Hume, I. D., Beiglböck, C., Ruf, T., Frey-Roos, F., Bruns, U., and Arnold, W. (2002). Seasonal changes in morphology and function of the gastrointestinal tract of free-living alpine marmots (Marmota marmota). J. Comp. Physiol. B 172, 197–207. doi: 10.1007/s00360-001-0240-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Humphries, M. M., Kramer, D. L., and Thomas, D. W. (2003a). The role of energy availability in mammalian hibernation: an experimental test in free-ranging eastern chipmunks. Physiol. Biochem. Zool. 76, 180–186. doi: 10.1086/367949

PubMed Abstract | CrossRef Full Text | Google Scholar

Humphries, M. M., Thomas, D. W., and Kramer, D. L. (2003b). The role of energy availability in mammalian hibernation: a cost-benefit approach. Physiol. Biochem. Zool. 76, 165–179. doi: 10.1086/367950

PubMed Abstract | CrossRef Full Text | Google Scholar

Humphries, M. M., Thomas, D. W., and Kramer, D. L. (2001). Torpor and digestion in food-storing hibernators. Physiol. Biochem. Zool. 74, 283–292. doi: 10.1086/319659

PubMed Abstract | CrossRef Full Text | Google Scholar

Ishigami, M., Hiraki, K., Umemura, K., Ogasawara, Y., Ishii, K., and Kimura, H. (2009). A source of hydrogen sulfide and a mechanism of its release in the brain. Antioxid. Redox. Signal. 11, 205–214. doi: 10.1089/ars.2008.2132

PubMed Abstract | CrossRef Full Text | Google Scholar

Jastroch, M., Giroud, S., Barrett, P., Geiser, F., Heldmaier, G., and Herwig, A. (2016). Seasonal control of mammalian energy balance: recent advances in the understanding of daily torpor and hibernation. J. Neuroendocrinol 28. doi: 10.1111/jne.12437

PubMed Abstract | CrossRef Full Text | Google Scholar

Johansson, B. W. (1996). The hibernator heart - Nature’s model of resistance to ventricular fibrillation. Cardiovasc. Res. 31, 826–832. doi: 10.1016/0008-6363(95)00192-1

CrossRef Full Text | Google Scholar

Johnson, D. C., Dean, D. R., Smith, A. D., and Johnson, M. K. (2005). Structure, function, and formation of biological iron-sulfur clusters. Annu. Rev. Biochem. 74, 247–281. doi: 10.1146/annurev.biochem.74.082803.133518

PubMed Abstract | CrossRef Full Text | Google Scholar

Jonasson, K. A., and Willis, C. K. (2011). Changes in body condition of hibernating bats support the thrifty female hypothesis and predict consequences for populations with white-nose syndrome. PLoS One 6:e21061. doi: 10.1371/journal.pone.0021061

PubMed Abstract | CrossRef Full Text | Google Scholar

Kart Gür, M., and Gür, H. (2015). Age and sex differences in hibernation patterns in free-living Anatolian ground squirrels. Mamm. Biol. 80, 265–272. doi: 10.1016/j.mambio.2015.02.006

CrossRef Full Text | Google Scholar

Kenagy, G. J., and Barnes, B. M. (1988). Seasonal reproductive patterns in four coexisting rodent species from the Cascade Mountains, Washington. J. Mammal. 69, 274–292. doi: 10.2307/1381378

CrossRef Full Text | Google Scholar

Khan, A. A., Schuler, M. M., Prior, M. G., Yong, S., Coppock, R. W., Florence, L. Z., et al. (1990). Effects of hydrogen sulfide exposure on lung mitochondrial respiratory chain enzymes in rats. Toxicol. Appl. Pharm. 103, 482–490. doi: 10.1016/0041-008X(90)90321-K

CrossRef Full Text | Google Scholar

Klug, B. J., and Brigham, R. M. (2015). Changes to metabolism and cell physiology that enable mammalian hibernation. Springer Sci. Rev. 3, 39–56. doi: 10.1007/s40362-015-0030-x

CrossRef Full Text | Google Scholar

Kobbe, S., Nowack, J., and Dausmann, K. H. (2014). Torpor is not the only option: seasonal variations of the thermoneutral zone in a small primate. J. Comp. Physiol. B 184, 789–797. doi: 10.1007/s00360-014-0834-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Körtner, G., and Geiser, F. (2009). The key to winter survival: daily torpor in a small arid-zone marsupial. Naturwissenschaften 96, 525–530. doi: 10.1007/s00114-008-0492-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Krilowicz, B. L. (1985). Ketone body metabolism in a ground squirrel during hibernation and fasting. Am. J. Physiol. Reg. Int. Comp. Physiol. 249, R462–R470. doi: 10.1152/ajpregu.1985.249.4.R462

PubMed Abstract | CrossRef Full Text | Google Scholar

Kronfeld-Schor, N., Richardson, C., Silvia, B. A., Kunz, T. H., and Widmaier, E. P. (2000). Dissociation of leptin secretion and adiposity during perhibernatory fattening in little brown bats. Am. J. Physiol. Reg. Int. Comp. Physiol. 279, R1277–R1281. doi: 10.1152/ajpregu.2000.279.4.R1277

PubMed Abstract | CrossRef Full Text | Google Scholar

Kurtz, C. C., and Carey, H. V. (2007). Seasonal changes in the intestinal immune system of hibernating ground squirrels. Dev. Comp. Immunol. 31, 415–428. doi: 10.1016/j.dci.2006.07.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Lafontan, M., and Viguerie, N. (2006). Role of adipokines in the control of energy metabolism: focus on adiponectin. Curr. Opin. Pharmacol. 6, 580–585. doi: 10.1016/j.coph.2006.08.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Landes, J., Pavard, S., Henry, P. Y., and Terrien, J. (2020). Flexibility is costly: hidden physiological damage from seasonal phenotypic transitions in Heterothermic species. Front. Physiol. 11:985. doi: 10.3389/Fphys.2020.00985

PubMed Abstract | CrossRef Full Text | Google Scholar

Languille, S., Blanc, S., Blin, O., Canale, C. I., Dal-Pan, A., Devau, G., et al. (2012). The grey mouse lemur: a non-human primate model for ageing studies. Ageing Res. Rev. 11, 150–162. doi: 10.1016/j.arr.2011.07.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Lee, H. K., Koh, S., Lo, D. C., and Marchuk, D. A. (2018). Neuronal IL-4R modulates neuronal apoptosis and cell viability during the acute phases of cerebral ischemia. FEBS J. 285, 2785–2798. doi: 10.1111/febs.14498

PubMed Abstract | CrossRef Full Text | Google Scholar

Lei, M., Dong, D., Mu, S., Pan, Y. H., and Zhang, S. Y. (2014). Comparison of brain transcriptome of the greater horseshoe bats (Rhinolophus ferrumequinum) in active and torpid episodes. PLoS One 9:e107746. doi: 10.1371/journal.pone.0107746

PubMed Abstract | CrossRef Full Text | Google Scholar

León-Espinosa, G., Regalado-Reyes, M., DeFelipe, J., and Muñoz, A. (2018). Changes in neocortical and hippocampal microglial cells during hibernation. Brain Struct. Funct. 223, 1881–1895. doi: 10.1007/s00429-017-1596-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Logan, S. M., and Storey, K. B. (2018). Pro-inflammatory AGE-RAGE signaling is activated during arousal from hibernation in ground squirrel adipose. PeerJ 6:e4911. doi: 10.7717/peerj.4911

PubMed Abstract | CrossRef Full Text | Google Scholar

Logan, S. M., Wu, C.-W., and Storey, K. B. (2019). The squirrel with the lagging eIF2: global suppression of protein synthesis during torpor. Comp. Biochem. Physiol. A 227, 161–171. doi: 10.1016/j.cbpa.2018.10.014

PubMed Abstract | CrossRef Full Text | Google Scholar

Lovegrove, B. G., and Genin, F. (2008). Torpor and hibernation in a basal placental mammal, the lesser hedgehog tenrec Echinops telfairi. J. Comp. Physiol. B 178, 691–698. doi: 10.1007/s00360-008-0257-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Lovegrove, B. G., Raman, J., and Perrin, M. R. (2001). Heterothermy in elephant shrews, Elephantulus spp. (Macroscelidea): daily torpor or hibernation? J. Comp. Physiol. B 171, 1–10. doi: 10.1007/s003600000139

PubMed Abstract | CrossRef Full Text | Google Scholar

Lu, A., Chu, C., Mulvihill, E., Wang, R., and Liang, W. (2019). ATP-sensitive K+ channels and mitochondrial permeability transition pore mediate effects of hydrogen sulfide on cytosolic Ca2+ homeostasis and insulin secretion in β-cells. Pflügers Arch. Eur. J. Physiol. 471, 1551–1564. doi: 10.1007/s00424-019-02325-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Luppi, M., Hitrec, T., Di Cristoforo, A., Squarcio, F., Stanzani, A., Occhinegro, A., et al. (2019). Phosphorylation and dephosphorylation of tau protein during synthetic torpor. Front. Neuroanat 13:57. doi: 10.3389/fnana.2019.00057

PubMed Abstract | CrossRef Full Text | Google Scholar

Luu, B. E., Lefai, E., Giroud, S., Swenson, J. E., Chazarin, B., Gauquelin-Koch, G., et al. (2020). MicroRNAs facilitate skeletal muscle maintenance and metabolic suppression in hibernating brown bears. J. Cell. Physiol. 235, 3984–3993. doi: 10.1002/jcp.29294

PubMed Abstract | CrossRef Full Text | Google Scholar

Lyman, C. P., and Chatfield, P. O. (1955). Physiology of hibernation in mammals. Physiol. Rev. 35, 403–425. doi: 10.1152/physrev.1955.35.2.403

PubMed Abstract | CrossRef Full Text | Google Scholar

Mahlert, B., Gerritsmann, H., Stalder, G., Ruf, T., Zahariev, A., Blanc, S., et al. (2018). Implications of being born late in the active season for growth, fattening, torpor use, winter survival and fecundity. eLife 7:e31225. doi: 10.7554/eLife.31225

PubMed Abstract | CrossRef Full Text | Google Scholar

Martin, B., Mattson, M. P., and Maudsley, S. (2006). Caloric restriction and intermittent fasting: Two potential diets for successful brain aging. Ageing Res. Rev. 5, 332–353. doi: 10.1016/j.arr.2006.04.002

PubMed Abstract | CrossRef Full Text | Google Scholar

Martin, G. M. (2010). Geographic distribution and historical occurrence of Dromiciops gliroides Thomas (Metatheria: Microbiotheria). J. Mammal. 91, 1025–1035. doi: 10.1644/09-mamm-a-347.1

CrossRef Full Text | Google Scholar

Masaki, T., Chiba, S., Yasuda, T., Tsubone, T., Kakuma, T., Shimomura, I., et al. (2003). Peripheral, But not central, administration ofadiponectin reduces visceral adiposity andupregulates the expression of uncoupling proteinin agouti yellow (Ay/a) obese mice. Diabetes 52, 2266–2273. doi: 10.2337/diabetes.52.9.2266

PubMed Abstract | CrossRef Full Text | Google Scholar

Mathers, K. E., McFarlane, S. V., Zhao, L., and Staples, J. F. (2017). Regulation of mitochondrial metabolism during hibernation by reversible suppression of electron transport system enzymes. J. Comp. Physiol. B 187, 227–234. doi: 10.1007/s00360-016-1022-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Michener, G. R. (1978). Effect of age and parity on weight gain and entry into hibernation in Richardson’s ground squirrels. Can. J. Zool. 56, 2573–2577. doi: 10.1139/z78-345

PubMed Abstract | CrossRef Full Text | Google Scholar

Mitchell, K. J., Pratt, R. C., Watson, L. N., Gibb, G. C., Llamas, B., Kasper, M., et al. (2014). Molecular phylogeny, biogeography, and habitat preference evolution of marsupials. Mol. Biol. Evol. 31, 2322–2330. doi: 10.1093/molbev/msu176

PubMed Abstract | CrossRef Full Text | Google Scholar

Miyake, S.-I., Wakita, H., Bernstock, J. D., Castri, P., Ruetzler, C., Miyake, J., et al. (2015). Hypophosphorylation of ribosomal protein S6 is a molecular mechanism underlying ischemic tolerance induced by either hibernation or preconditioning. J. Neurochem. 135, 943–957. doi: 10.1111/jnc.13368

PubMed Abstract | CrossRef Full Text | Google Scholar

Módis, K., Bos, E. M., Calzia, E., van Goor, H., Coletta, C., Papapetropoulos, A., et al. (2014). Regulation of mitochondrial bioenergetic function by hydrogen sulfide. Part II. Pathophysiological and therapeutic aspects. Br. J. Pharmacol. 171, 2123–2146. doi: 10.1111/bph.12368

PubMed Abstract | CrossRef Full Text | Google Scholar

Módis, K., Coletta, C., Erdélyi, K., Papapetropoulos, A., and Szabo, C. (2013). Intramitochondrial hydrogen sulfide production by 3-mercaptopyruvate sulfurtransferase maintains mitochondrial electron flow and supports cellular bioenergetics. FASEB J. 27, 601–611. doi: 10.1096/fj.12-216507

PubMed Abstract | CrossRef Full Text | Google Scholar

Mokrasch, L. C., Grady, H. J., and Grisolia, S. (1960). Thermogenic and adaptive mechanisms in hibernation and arousal from hibernation. Am. J. Physiol. Legacy Content 199, 945–949. doi: 10.1152/ajplegacy.1960.199.5.945

PubMed Abstract | CrossRef Full Text | Google Scholar

Morin, P. Jr., Ni, Z., McMullen, D. C., and Storey, K. B. (2008). Expression of Nrf2 and its downstream gene targets in hibernating 13-lined ground squirrels, Spermophilus tridecemlineatus. Mol. Cell. Biochem. 312, 121–129. doi: 10.1007/s11010-008-9727-3

PubMed Abstract | CrossRef Full Text | Google Scholar

Morin, P. Jr., and Storey, K. B. (2007). Antioxidant defense in hibernation: cloning and expression of peroxiredoxins from hibernating ground squirrels, Spermophilus tridecemlineatus. Arch. Biochem. Biophys. 461, 59–65. doi: 10.1016/j.abb.2007.01.035

PubMed Abstract | CrossRef Full Text | Google Scholar

Muleme, H. M., Walpole, A. C., and Staples, J. F. (2006). Mitochondrial metabolism in hibernation: metabolic suppression, temperature effects, and substrate preferences. Physiol. Biochem. Zool. 79, 474–483. doi: 10.1086/501053

PubMed Abstract | CrossRef Full Text | Google Scholar

Muñoz-Pedreros, A., Lang, B. K., Bretos, M., and Meserve, P. L. (2005). Reproduction and development of Dromiciops gliroides (Marsupialia: Microbiotheriidae) in temperate rainforests of southern Chile. Gayana 69, 225–233. doi: 10.4067/S0717-65382005000200002

PubMed Abstract | CrossRef Full Text | Google Scholar

Mustafa, A. K., Gadalla, M. M., Sen, N., Kim, S., Mu, W., Gazi, S. K., et al. (2009). H2S signals through protein S-sulfhydration. Sci. Signal. 2:ra72. doi: 10.1126/scisignal.2000464

PubMed Abstract | CrossRef Full Text | Google Scholar

Nagahara, N., Ito, T., Kitamura, H., and Nishino, T. (1998). Tissue and subcellular distribution of mercaptopyruvate sulfurtransferase in the rat: confocal laser fluorescence and immunoelectron microscopic studies combined with biochemical analysis. Histochem. Cell Biol. 110, 243–250. doi: 10.1007/s004180050286

PubMed Abstract | CrossRef Full Text | Google Scholar

Nespolo, R. F., Fontúrbel, F. E., Mejias, C., Contreras, R., Gutierrez, P., Oda, E., et al. (2020). A mesocosm experiment in ecological physiology: adaptive modulation of energy budget in a hibernating marsupial under chronic caloric restriction. bioRxiv [Preprint]. doi: 10.1101/2020.06.05.136028

CrossRef Full Text | Google Scholar

Nespolo, R. F., Gaitan-Espitia, J. D., Quintero-Galvis, J. F., Fernandez, F. V., Silva, A. X., Molina, C., et al. (2018). A functional transcriptomic analysis in the relict marsupial Dromiciops gliroides reveals adaptive regulation of protective functions during hibernation. Mol. Ecol. 27, 4489–4500. doi: 10.1111/mec.14876

PubMed Abstract | CrossRef Full Text | Google Scholar

Nespolo, R. F., Verdugo, C., Cortes, P. A., and Bacigalupe, L. D. (2010). Bioenergetics of torpor in the Microbiotherid marsupial, Monito del Monte (Dromiciops gliroides): the role of temperature and food availability. J. Comp. Physiol. B 180, 767–773. doi: 10.1007/s00360-010-0449-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Ni, Z. L., and Storey, K. B. (2010). Heme oxygenase expression and Nrf2 signaling during hibernation in ground squirrels. Can. J. Physiol. Pharmacol. 88, 379–387. doi: 10.1139/Y10-017

PubMed Abstract | CrossRef Full Text | Google Scholar

Nizielski, S. E., Billington, C. J., and Levine, A. S. (1989). Brown fat GDP binding and circulating metabolites during hibernation and arousal. Am. J. Physiol. Reg. Int. Comp. Physiol. 257, R536–R541. doi: 10.1152/ajpregu.1989.257.3.R536

PubMed Abstract | CrossRef Full Text | Google Scholar

Noiret, A., Puch, L., Riffaud, C., Costantini, D., Riou, J. F., Aujard, F., et al. (2020). Sex-specific response to caloric restriction after reproductive investment in Microcebus murinus: an integrative approach. Front. Physiol. 11:506. doi: 10.3389/fphys.2020.00506

PubMed Abstract | CrossRef Full Text | Google Scholar

Nowack, J., Giroud, S., Arnold, W., and Ruf, T. (2017). Muscle non-shivering thermogenesis and its role in the evolution of endothermy. Front. Physiol. 8:889. doi: 10.3389/fphys.2017.00889

PubMed Abstract | CrossRef Full Text | Google Scholar

Nowack, J., Levesque, D. L., Reher, S., and Dausmann, K. H. (2020). Variable climates lead to varying phenotypes: “Weird” mammalian torpor and lessons from non-holarctic species. Front. Ecol. Evol. 8:60. doi: 10.3389/fevo.2020.00060

CrossRef Full Text | Google Scholar

Nowack, J., Tarmann, I., Hoelzl, F., Smith, S., Giroud, S., and Ruf, T. (2019). Always a price to pay: hibernation at low temperatures comes with a trade-off between energy savings and telomere damage. Biol. Lett. 15:20190466. doi: 10.1098/rsbl.2019.0466

PubMed Abstract | CrossRef Full Text | Google Scholar

Ormseth, O. A., Nicolson, M., Pelleymounter, M. A., and Boyer, B. B. (1996). Leptin inhibits prehibernation hyperphagia and reduces body weight in arctic ground squirrels. Am. J. Physiol. Reg. Int. Comp. Physiol. 271, R1775–R1779. doi: 10.1152/ajpregu.1996.271.6.R1775

PubMed Abstract | CrossRef Full Text | Google Scholar

Orr, A. L., Lohse, L. A., Drew, K. L., and Hermes-Lima, M. (2009). Physiological oxidative stress after arousal from hibernation in Arctic ground squirrel. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 153, 213–221. doi: 10.1016/j.cbpa.2009.02.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Ou, J., Ball, J. M., Luan, Y., Zhao, T., Miyagishima, K. J., Xu, Y., et al. (2018). iPSCs from a hibernator provide a platform for studying cold adaptation and its potential medical applications. Cell 173, 851.e16–863.e16. doi: 10.1016/j.cell.2018.03.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Papu John, A. S., Kundu, S., Pushpakumar, S., Amin, M., Tyagi, S. C., and Sen, U. (2019). Hydrogen sulfide inhibits Ca2+-induced mitochondrial permeability transition pore opening in type-1 diabetes. Am. J. Physiol. Endocrinol. Metab. 317, E269–E283. doi: 10.1152/ajpendo.00251.2018

PubMed Abstract | CrossRef Full Text | Google Scholar

Patterson, R. E., and Sears, D. D. (2017). Metabolic effects of intermittent fasting. Annu. Rev. Nutr. 37, 371–393. doi: 10.1146/annurev-nutr-071816-064634

PubMed Abstract | CrossRef Full Text | Google Scholar

Perret, M. (1997). Change in photoperiodic cycle affects life spanin a prosimian primate (Microcebus murinus). J. Biol. Rhythms 12, 136–145. doi: 10.1177/074873049701200205

PubMed Abstract | CrossRef Full Text | Google Scholar

Perret, M., and Aujard, F. (2001). Regulation by photoperiod of seasonal changes inBody mass and reproductive function in GrayMouse Lemurs (Microcebus murinus): differential responses by sex. Int. J. Primatol. 22, 5–24. doi: 10.1023/A:1026457813626

CrossRef Full Text | Google Scholar

Popov, V. I., Bocharova, L. S., and Bragin, A. G. (1992). Repeated changes of dendritic morphology in the hippocampus of ground squirrels in the course of hibernation. Neuroscience 48, 45–51. doi: 10.1016/0306-4522(92)90336-Z

CrossRef Full Text | Google Scholar

Popova, N. K., and Koryakina, L. A. (1981). Seasonal changes in pituitary-adrenal reactivity in hibernating spermophiles. Endocrinol. Exp. 15, 269–276.

Google Scholar

Prendergast, B. J., Freeman, D. A., Zucker, I., and Nelson, R. J. (2002). Periodic arousal from hibernation is necessary for initiation of immune responses in ground squirrels. Am. J. Physiol. Reg. Int. Comp. Physiol. 282, R1054–R1082. doi: 10.1152/ajpregu.00562.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

Rauch, J. C., and Behrisch, H. W. (1981). Ketone bodies: a source of energy during hibernation. Can. J. Zool. 59, 754–760. doi: 10.1139/z81-108

CrossRef Full Text | Google Scholar

Revsbech, I. G., Shen, X., Chakravarti, R., Jensen, F. B., Thiel, B., Evans, A. L., et al. (2014). Hydrogen sulfide and nitric oxide metabolites in the blood of free-ranging brown bears and their potential roles in hibernation. Free Rad. Biol. Med. 73, 349–357. doi: 10.1016/j.freeradbiomed.2014.05.025

PubMed Abstract | CrossRef Full Text | Google Scholar

Romagnani, P., Remuzzi, G., Glassock, R., Levin, A., Jager, K. J., Tonelli, M., et al. (2017). Chronic kidney disease. Nat. Rev. Dis. Primers 3:17088. doi: 10.1038/nrdp.2017.88

PubMed Abstract | CrossRef Full Text | Google Scholar

Roshan, R., Shridhar, S., Sarangdhar, M. A., Banik, A., Chawla, M., Garg, M., et al. (2014). Brain-specific knockdown of miR-29 results in neuronal cell death and ataxia in mice. RNA 20, 1287–1297. doi: 10.1261/rna.044008.113

PubMed Abstract | CrossRef Full Text | Google Scholar

Rouble, A. N., Hefler, J., Mamady, H., Storey, K. B., and Tessier, S. N. (2013). Anti-apoptotic signaling as a cytoprotective mechanism in mammalian hibernation. PeerJ 1:e29. doi: 10.7717/peerj.29

PubMed Abstract | CrossRef Full Text | Google Scholar

Ruf, T., and Arnold, W. (2008). Effects of polyunsaturated fatty acids on hibernation and torpor: a review and hypothesis. Am. J. Physiol. Reg. Int. Comp. Physiol. 294, R1044–R1052. doi: 10.1152/ajpregu.00688.2007

PubMed Abstract | CrossRef Full Text | Google Scholar

Ruf, T., Bieber, C., and Turbill, C. (2012). “Survival, aging, and life-history tactics in mammalian hibernators,” in Living in a Seasonal World. Thermoregulatory and Metabolic Adaptations, eds T. Ruf, C. Bieber, W. Arnold, and E. Millesi (Heidelberg: Springer Verlag), 123–132. doi: 10.1007/978-3-642-28678-0_11

CrossRef Full Text | Google Scholar

Ruf, T., and Geiser, F. (2015). Daily torpor and hibernation in birds and mammals. Biol. Rev. 90, 891–926. doi: 10.1111/brv.12137

PubMed Abstract | CrossRef Full Text | Google Scholar

Sarnak, M. J., Amann, K., Bangalore, S., Cavalcante, J. L., Charytan, D. M., Craig, J. C., et al. (2019). Chronic kidney disease and coronary artery disease: JACC State-of-the-art review. J. Am. Coll. Cardiol. 74, 1823–1838. doi: 10.1016/j.jacc.2019.08.1017

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmid, J. (1999). Sex-specific differences in activity patterns and fattening in the gray mouse lemur (Microcebus murinus) in Madagascar. J. Mammal. 80, 749–757. doi: 10.2307/1383244

CrossRef Full Text | Google Scholar

Schmid, J., and Ganzhorn, J. U. (2009). Optional strategies for reduced metabolism in gray mouse lemurs. Naturwissenschaften 96, 737–741. doi: 10.1007/s00114-009-0523-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Schmid, J., and Kappeler, P. M. (1998). Fluctuating sexual dimorphism and differential hibernation by sex in a primate, the gray mouse lemur (Microcebus murinus). Behav. Ecol. Sociobiol. 43, 125–132. doi: 10.1007/s002650050474

CrossRef Full Text | Google Scholar

Schwartz, C., Hampton, M., and Andrews, M. T. (2013). Seasonal and regional differences in gene expression in the brain of a hibernating mammal. PLoS One 8:e58427. doi: 10.1371/journal.pone.0058427

PubMed Abstract | CrossRef Full Text | Google Scholar

Shaposhnikov, M., Proshkina, E., Koval, L., Zemskaya, N., Zhavoronkov, A., and Moskalev, A. (2018). Overexpression of CBS and CSE genes affects lifespan, stress resistance and locomotor activity in Drosophila melanogaster. Aging 10, 3260–3272. doi: 10.18632/aging.101630

PubMed Abstract | CrossRef Full Text | Google Scholar

Shibuya, N., Mikami, Y., Kimura, Y., Nagahara, N., and Kimura, H. (2009). Vascular endothelium expresses 3-mercaptopyruvate sulfurtransferase and produces hydrogen sulfide. J. Biochem. 146, 623–626. doi: 10.1093/jb/mvp111

PubMed Abstract | CrossRef Full Text | Google Scholar

Shivatcheva, T. M., Ankov, V. K., and Hadjioloff, A. I. (1988). Circannual fluctuations of the serum cortisol in the European ground squirrel, Citellus citellus L. Comp. Biochem. Physiol. A 90, 515–518. doi: 10.1016/0300-9629(88)90229-0

CrossRef Full Text | Google Scholar

Siutz, C., Franceschini, C., and Millesi, E. (2016). Sex and age differences in hibernation patterns of common hamsters: adult females hibernate for shorter periods than males. J. Comp. Physiol. B 186, 801–811. doi: 10.1007/s00360-016-0995-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Snapp, B. D., and Heller, H. C. (1981). Suppression of metabolism during hibernation in ground squirrels (Citellus lateralis). Physiol. Zool. 54, 297–307. doi: 10.1086/physzool.54.3.30159944

CrossRef Full Text | Google Scholar

Song, X., and Geiser, F. (1997). Daily torpor and energy expenditure in sminthopsis macroura: interactions between food and water availability and temperature. Physiol. Zool. 70, 331–337. doi: 10.1086/639610

PubMed Abstract | CrossRef Full Text | Google Scholar

Spindel, O. N., World, C., and Berk, B. C. (2012). Thioredoxin interacting protein: redox dependent and independent regulatory mechanisms. Antioxid. Redox. Signal. 16, 587–596. doi: 10.1089/ars.2011.4137

PubMed Abstract | CrossRef Full Text | Google Scholar

Staples, J. F. (2011). “Metabolic flexibility: hibernation, torpor, and estivation,” in Comprehensive Physiology, ed. D. M. Pollock (Hoboken, NJ: John Wiley & Sons, Inc), 737–771. doi: 10.1002/cphy.c140064

PubMed Abstract | CrossRef Full Text | Google Scholar

Storey, K. B. (2015). The gray mouse lemur: a model for studies of primate metabolic rate depression. Genomics Proteomics Bioinformatics 13, 77–80. doi: 10.1016/j.gpb.2015.06.001

PubMed Abstract | CrossRef Full Text | Google Scholar

Storey, K. B., and Storey, J. M. (2004). Metabolic rate depression in animals: transcriptional and translational controls. Biol. Rev. 79, 207–233. doi: 10.1017/S1464793103006195

PubMed Abstract | CrossRef Full Text | Google Scholar

Storey, K. B., and Storey, J. M. (2007). Tribute to P. L. Lutz: putting life on ‘pause’ - molecular regulation of hypometabolism. J. Exp. Biol. 210, 1700–1714. doi: 10.1242/jeb.02716

PubMed Abstract | CrossRef Full Text | Google Scholar

Stumpfel, S., Bieber, C., Blanc, S., Ruf, T., and Giroud, S. (2017). Differences in growth rates and pre-hibernation body mass gain between early and late-born juvenile garden dormice. J. Comp. Physiol. B 187, 253–263. doi: 10.1007/s00360-016-1017-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Szabo, C. (2018). A timeline of hydrogen sulfide (H2S) research: from environmental toxin to biological mediator. Biochem. Pharmacol. 149, 5–19. doi: 10.1016/j.bcp.2017.09.010

PubMed Abstract | CrossRef Full Text | Google Scholar

Takahashi, T. M., Sunagawa, G. A., Soya, S., Abe, M., Sakurai, K., Ishikawa, K., et al. (2020). A discrete neuronal circuit induces a hibernation-lile state in rodents. Nature 583, 109–114. doi: 10.1038/s41586-020-2163-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Talaei, F., Bouma, H. R., Hylkema, M. N., Strijkstra, A. M., Boerema, A. S., Schmidt, M., et al. (2012). The role of endogenous H2S formation in reversible remodeling of lung tissue during hibernation in the Syrian hamster. J. Exp. Biol. 215, 2912–2919. doi: 10.1242/jeb.067363

PubMed Abstract | CrossRef Full Text | Google Scholar

Talaei, F., Bouma, H. R., Van der Graaf, A. C., Strijkstra, A. M., Schmidt, M., and Henning, R. H. (2011a). Serotonin and dopamine protect from Hypothermia/Rewarming damage through the CBS/H2S Pathway. PLoS One 6:e22568. doi: 10.1371/journal.pone.0022568

PubMed Abstract | CrossRef Full Text | Google Scholar

Talaei, F., Hylkema, M. N., Bouma, H. R., Boerema, A. S., Strijkstra, A. M., Henning, R. H., et al. (2011b). Reversible remodeling of lung tissue during hibernation in the Syrian hamster. J. Exp. Biol. 214(Pt 8), 1276–1282. doi: 10.1242/jeb.052704

PubMed Abstract | CrossRef Full Text | Google Scholar

Tashima, L. S., Adelstein, S. J., and Lyman, C. P. (1970). Radioglucose utilization by active, hibernating, and arousing ground squirrels. Am. J. Physiol. Legacy Content 218, 303–309. doi: 10.1152/ajplegacy.1970.218.1.303

PubMed Abstract | CrossRef Full Text | Google Scholar

Teng, H., Wu, B., Zhao, K., Yang, G., Wu, L., and Wang, R. (2013). Oxygen-sensitive mitochondrial accumulation of cystathionine β-synthase mediated by Lon protease. Proc. Natl. Acad. Sci. U.S.A. 110, 12679–12684. doi: 10.1073/pnas.1308487110

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Ambid, L., Nibbelink, M., Saint-Charles, A., and Aujard, F. (2010a). Non-shivering thermogenesis activation and maintenance in the aging gray mouse lemur (Microcebus murinus). Exp. Gerontol. 45, 442–448. doi: 10.1016/j.exger.2010.03.013

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Perret, M., and Aujard, F. (2010b). Gender markedly modulates behavioral thermoregulation in a non-human primate species, the mouse lemur (Microcebus murinus). Physiol. Behav. 101, 469–473. doi: 10.1016/j.physbeh.2010.07.012

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Gaudubois, M., Champeval, D., Zaninotto, V., Roger, L., Riou, J. F., et al. (2017). Metabolic and genomic adaptations to winter fattening in a primate species, the grey mouse lemur (Microcebus murinus). Int. J. Obes. 42, 211–230. doi: 10.1038/ijo.2017.195

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Perret, M., and Aujard, F. (2011). Behavioral thermoregulation in mammals: a review. Front. Biosci. 16:1428–1444. doi: 10.2741/3797

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Zahariev, A., Blanc, S., and Aujard, F. (2009). Impaired control of body cooling during heterothermia represents the major energetic constraint in an aging non-human primate exposed to cold. PLoS One 4:e7587. doi: 10.1371/journal.pone.0007587

PubMed Abstract | CrossRef Full Text | Google Scholar

Terrien, J., Zizzari, P., Bluet-Pajot, M. T., Henry, P. Y., Perret, M., Epelbaum, J., et al. (2008). Effects of age on thermoregulatory responses during cold exposure in a nonhuman primate. Microcebus murinus. Am. J. Physiol. Reg. Int. Comp. Physiol. 295, R696–R703. doi: 10.1152/ajpregu.00629.2007

PubMed Abstract | CrossRef Full Text | Google Scholar

Tessier, S. N., Katzenback, B. A., Pifferi, F., Perret, M., and Storey, K. B. (2015a). Cytokine and antioxidant regulation in the intestine of the gray mouse lemur (Microcebus murinus) during torpor. Genomics Proteomics Bioinformatics 13, 127–135. doi: 10.1016/j.gpb.2015.03.005

PubMed Abstract | CrossRef Full Text | Google Scholar

Tessier, S. N., Zhang, J., Biggar, K. K., Wu, C. W., Pifferi, F., Perret, M., et al. (2015b). Regulation of the PI3K/AKT pathway and fuel utilization during primate torpor in the grey mouse lemur. Microcebus murinus. Genomics Proteomics Bioinformatics 13, 91–102. doi: 10.1016/j.gpb.2015.03.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Tessier, S. N., Wu, C.-W., and Storey, K. B. (2019). Molecular control of protein synthesis, glucose metabolism, and apoptosis in the brain of hibernating thirteen-lined ground squirrels. Biochem. Cell Biol. 97, 536–544. doi: 10.1139/bcb-2018-0256

PubMed Abstract | CrossRef Full Text | Google Scholar

Turbill, C., Bieber, C., and Ruf, T. (2011). Hibernation is associated with increased survival and the evolution of slow life histories among mammals. Proc. R. Soc. B 278, 3355–3363. doi: 10.1098/rspb.2011.0190

PubMed Abstract | CrossRef Full Text | Google Scholar

Turbill, C., Ruf, T., Smith, S., and Bieber, C. (2013). Seasonal variation in telomere length of a hibernating rodent. Biol. Lett. 9:20121095. doi: 10.1098/rsbl.2012.1095

PubMed Abstract | CrossRef Full Text | Google Scholar

Twente, J. W., and Twente, J. A. (1967). Concentrations of d-glucose in the blood of Citellus lateralis after known intervals of hbernating priods. J. Mammal. 48, 381–386. doi: 10.2307/1377770

CrossRef Full Text | Google Scholar

Valladares-Gómez, A., Celis-Diez, J. L., Sepúlveda-Rodríguez, C., Inostroza-Michael, O., Hernández, C. E., and Palma, R. E. (2019). genetic diversity, population structure, and migration scenarios of the marsupial “Monito del Monte” in south-central chile. J. Hered. 110, 651–661. doi: 10.1093/jhered/esz049

PubMed Abstract | CrossRef Full Text | Google Scholar

Van Breukelen, F., and Martin, S. L. (2002). Invited review: molecular adaptations in mammalian hibernators: unique adaptations or generalized responses? J. Appl. Physiol 92, 2640–2647. doi: 10.1152/japplphysiol.01007.2001

PubMed Abstract | CrossRef Full Text | Google Scholar

van Breukelen, F., and Martin, S. L. (2015). The hibernation continuum: physiological and molecular aspects of metabolic plasticity in mammals. Physiology 30, 273–281. doi: 10.1152/physiol.00010.2015

PubMed Abstract | CrossRef Full Text | Google Scholar

Vander Wall, S. B. (1990). Food Hoarding in Animals. Chicago, IL: The University of Chicago Press.

Google Scholar

Vermillion, K. L., Jagtap, P., Johnson, J. E., Griffin, T. J., and Andrews, M. T. (2015). Characterizing cardiac molecular mechanisms of mammalian hibernation via quantitative proteogenomics. J. Proteome Res. 14, 4792–4804. doi: 10.1021/acs.jproteome.5b00575

PubMed Abstract | CrossRef Full Text | Google Scholar

Villain, N., Picq, J. L., Aujard, F., and Pifferi, F. (2016). Body mass loss correlates with cognitive performance in primates under acute caloric restriction conditions. Behav. Brain Res. 305, 157–163. doi: 10.1016/j.bbr.2016.02.037

PubMed Abstract | CrossRef Full Text | Google Scholar

Vuarin, P., Dammhahn, M., Kappeler, P. M., and Henry, P. Y. (2015). When to initiate torpor use? Food availability times the transition to winter phenotype in a tropical heterotherm. Oecologia 179, 43–53. doi: 10.1007/s00442-015-3328-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, L. C. H. (1978). “Energetic and field aspects of mammalian torpor: the Richardson’s ground squirrel,” in Strategies in Cold: Natural Torpidity and Thermogenesis, eds L. C. H. Wang and J. W. Hudson (New York, NY: Academic Press), 109–145. doi: 10.1016/b978-0-12-734550-5.50009-0

CrossRef Full Text | Google Scholar

Wassmer, T. (2004). Body temperature and above-ground patterns during hibernation in European hamsters (Cricetus cricetus L.). J. Zool. 262, 281–288. doi: 10.1017/S0952836903004643

CrossRef Full Text | Google Scholar

Waßmer, T., and Wollnik, F. (1997). Timing of torpor bouts during hibernation in European hamsters (Cricetus cricetus L.). J. Comp. Physiol. B 167, 270–279. doi: 10.1007/s003600050074

PubMed Abstract | CrossRef Full Text | Google Scholar

Wei, Y., Gong, L., Fu, W., Xu, S., Wang, Z., Zhang, J., et al. (2018a). Unexpected regulation pattern of the IKKβ/NF-κB/MuRF1 pathway with remarkable muscle plasticity in the Daurian ground squirrel (Spermophilus dauricus). J. Cell. Physiol. 233, 8711–8722. doi: 10.1002/jcp.26751

PubMed Abstract | CrossRef Full Text | Google Scholar

Wei, Y., Zhang, J., Xu, S., Peng, X., Yan, X., Li, X., et al. (2018b). Controllable oxidative stress and tissue specificity in major tissues during the torpor–arousal cycle in hibernating Daurian ground squirrels. Open Biol. 8:180068. doi: 10.1098/rsob.180068

PubMed Abstract | CrossRef Full Text | Google Scholar

Weitten, M., Oudart, H., and Habold, C. (2016). Maintenance of a fully functional digestive system during hibernation in the European hamster, a food-storing hibernator. Comp. Biochem. Physiol. A 193, 45–51. doi: 10.1016/j.cbpa.2016.01.006

PubMed Abstract | CrossRef Full Text | Google Scholar

Weitten, M., Robin, J. P., Oudart, H., Pevet, P., and Habold, C. (2013). Hormonal changes and energy substrate availability during the hibernation cycle of Syrian hamsters. Horm. Behav. 64, 611–617. doi: 10.1016/j.yhbeh.2013.08.015

PubMed Abstract | CrossRef Full Text | Google Scholar

Wolf, B. O., McKechnie, A. E., Schmitt, C. J., Czenze, Z. J., Johnson, A. B., and Witt, C. C. (2020). Extreme and variable torpor among high-elevation Andean hummingbird species. Biol. Lett. 16:20200428. doi: 10.1098/rsbl.2020.0428

PubMed Abstract | CrossRef Full Text | Google Scholar

Wolf, M., van Doorn, G. S., Leimar, O., and Weissing, F. J. (2007). Life-history trade-offs favour the evolution of animal personalities. Nature 447, 581–584. doi: 10.1038/nature05835

PubMed Abstract | CrossRef Full Text | Google Scholar

Wollnik, F., and Schmidt, B. (1995). Seasonal and daily rhythms of body temperature in the European hamster (Cricetus cricetus) under semi-natural conditions. J. Comp. Physiol. B 165, 171–182. doi: 10.1007/BF00260808

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, C. W., Biggar, K. K., and Storey, K. B. (2014). Expression profiling and structural characterization of MicroRNAs in adipose tissues of hibernating ground squirrels. Genomics Proteomics Bioinformatics 12, 284–291. doi: 10.1016/j.gpb.2014.08.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, C.-W., and Storey, K. B. (2014). FoxO3a-mediated activation of stress responsive genes during early torpor in a mammalian hibernator. Mol. Cell. Biochem. 390, 185–195. doi: 10.1007/s11010-014-1969-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, G., Wu, L., Jiang, B., Yang, W., Qi, J., Cao, K., et al. (2008). H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase. Science 322, 587–590. doi: 10.1126/science.1162667

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, G., Zhao, K., Ju, Y., Mani, S., Cao, Q., Puukila, S., et al. (2012). Hydrogen sulfide protects against cellular senescence via S-Sulfhydration of Keap1 and Activation of Nrf2. Antioxid. Redox. Signal. 18, 1906–1919. doi: 10.1089/ars.2012.4645

PubMed Abstract | CrossRef Full Text | Google Scholar

Yao, L.-L., Huang, X.-W., Wang, Y.-G., Cao, Y.-X., Zhang, C.-C., and Zhu, Y.-C. (2010). Hydrogen sulfide protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by preventing GSK-3β-dependent opening of mPTP. Am. J. Physiol. Heart Circ. Physiol. 298, H1310–H1319. doi: 10.1152/ajpheart.00339.2009

PubMed Abstract | CrossRef Full Text | Google Scholar

Yin, Q., Ge, H., Liao, C.-C., Liu, D., Zhang, S., and Pan, Y.-H. (2016). Antioxidant defenses in the brains of bats during hibernation. PLoS One 11:e0152135. doi: 10.1371/journal.pone.0152135

PubMed Abstract | CrossRef Full Text | Google Scholar

Yoshioka, J., and Lee, R. T. (2014). Thioredoxin-interacting protein and myocardial mitochondrial function in ischemia-reperfusion injury. Trends Cardiovasc. Med. 24, 75–80. doi: 10.1016/j.tcm.2013.06.007

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, J., Tessier, S. N., Biggar, K. K., Wu, C. W., Pifferi, F., Perret, M., et al. (2015). Regulation of torpor in the gray mouse lemur: transcriptional and translational controls and role of AMPK signaling. Genomics Proteomics Bioinformatics 13, 103–110. doi: 10.1016/j.gpb.2015.03.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, K. X., D’Souza, S., Upton, B. A., Kernodle, S., Vemaraju, S., Nayak, G., et al. (2020). Violet-light suppression o thermogenesis by opsin 5 hypothalamic neurons. Nature 585, 420–425. doi: 10.1038/s41586-020-2683-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, K., Ju, Y., Li, S., Altaany, Z., Wang, R., and Yang, G. (2014). S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO Rep. 15, 792–800. doi: 10.1002/embr.201338213

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhao, W., Zhang, J., Lu, Y., and Wang, R. (2001). The vasorelaxant effect of H2S as a novel endogenous gaseous KATP channel opener. EMBO J. 20, 6008–6016. doi: 10.1093/emboj/20.21.6008

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhou, J., and Zhang, J. (2014). Identification of miRNA-21 and miRNA-24 in plasma as potential early stage markers of acute cerebral infarction. Mol. Med. Rep. 10, 971–976. doi: 10.3892/mmr.2014.2245

PubMed Abstract | CrossRef Full Text | Google Scholar

Zuhra, K., Augsburger, F., Majtan, T., and Szabo, C. (2020). Cystathionine-β-synthase: molecular regulation and pharmacological inhibition. Biomolecules 10:697. doi: 10.3390/biom10050697

PubMed Abstract | CrossRef Full Text | Google Scholar