AUTHOR=Diniz Tainá Gomes , Silva Alexandre Sérgio , dos Santos Nunes Mayara Karla , Ribeiro Mateus Duarte , Filho João Modesto , do Nascimento Rayner Anderson Ferreira , Gomes Cecília Neta Alves Pegado , Evangelista Isabella Wanderley de Queiroga , de Oliveira Naila Francis Paulo , Persuhn Darlene Camati 

TITLE=Physical Activity Level Influences MTHFR Gene Methylation Profile in Diabetic Patients

JOURNAL=Frontiers in Physiology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.618672

DOI=10.3389/fphys.2020.618672

ISSN=1664-042X

ABSTRACT=<sec><title>Introduction</title><p><italic>MTHFR</italic> methylation status is associated with microvascular complications in diabetes, but the factors influencing this profile remain unknown.</p></sec><sec><title>Objective</title><p>The aim of this study was to evaluate the influence of physical activity level and nutritional status on the methylation profile of the <italic>MTHFR</italic> gene in patients with type 2 diabetes mellitus (T2DM).</p></sec><sec><title>Methods</title><p>A total of 111 patients, 43 men and 68 women diagnosed with DM (7.0 ± 2.3 years), answered the International Physical Activity Questionnaire (IPAQ) and underwent blood collection for biochemical analysis, DNA extraction, and <italic>MTHFR</italic> gene methylation profile determination.</p></sec><sec><title>Result</title><p>The comparison of the methylation pattern showed that the partially methylated profile predominates in the insufficiently active group (85%), which does not occur in the sufficiently active group (54%) (<italic>p</italic> = 0.012). No differences were found in the nutritional status comparison. Logistic regression including overweight, waist circumference, gender, age, time of DM, hypertension, dyslipidemia, smoking, alcoholism, and family DM revealed that the association of the level of physical activity with methylation profile proved to be independent of these confounding variables. Considering the partially methylated profile as a result, being physically inactive favors the partially methylated <italic>MTHFR</italic> pattern in patients with DM.</p></sec><sec><title>Conclusion</title><p>We concluded that insufficient physical activity is associated with partially methylated pattern of <italic>MTHFR</italic> promoter.</p></sec>