AUTHOR=Jin Jisu , Li Youzhi , Zhou Zhongshi , Zhang Hong , Guo Jianying , Wan Fanghao 

TITLE=Heat Shock Factor Is Involved in Regulating the Transcriptional Expression of Two Potential Hsps (AhHsp70 and AhsHsp21) and Its Role in Heat Shock Response of Agasicles hygrophila

JOURNAL=Frontiers in Physiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.562204

DOI=10.3389/fphys.2020.562204

ISSN=1664-042X

ABSTRACT=<p>Heat shock proteins are molecular chaperones that are involved in numerous normal cellular processes and stress responses, and heat shock factors are transcriptional activators of heat shock proteins. Heat shock factors and heat shock proteins are coordinated in various biological processes. The regulatory function of heat shock factors in the expression of genes encoding heat shock proteins (Hsps) has been documented in some model insects, however, the role of transcription factors in modulating Hsps in other insects is still limited. In this study, one heat shock factor gene (<italic>AhHsf</italic>) was isolated and its two potential target genes (<italic>AhHsp70</italic> and <italic>AhsHsp21</italic>) were confirmed from <italic>Agasicles hygrophila</italic>. <italic>AhHsf</italic> sequence analysis indicated that it belongs to the Hsfs gene family. RT-qPCR showed that expression levels of heat shock factors and of two heat shock proteins significantly increased under heat stress. Injection with double-stranded <italic>Hsf</italic> RNA in freshly emerged adult beetles significantly inhibited expression of <italic>AhHsp70</italic> and <italic>AhsHsp21</italic>, shortened the adult survival, drastically reduced egg production, and ultimately led to a decrease in fecundity. RNA interference (RNAi)-mediated suppression of <italic>AhHsp70</italic> or <italic>AhsHsp21</italic> expression also significantly affected expression of <italic>AhHsf</italic>. Our findings revealed a potential transcriptional function of AhHsf to regulate expression of <italic>AhHsp70</italic> and <italic>AhsHsp21</italic>, which may play a key role in <italic>A. hygrophila</italic> thermotolerance. Our results improve our understanding of the molecular mechanisms of the AhHsf - AhHsps signaling pathway in <italic>A. hygrophila.</italic></p>