AUTHOR=Curnow Andrew C. , Gonsalez Sabrina R. , Gogulamudi Venkateswara R. , Visniauskas Bruna , Simon Eric E. , Gonzalez Alexis A. , Majid Dewan S. A. , Lara Lucienne S. , Prieto Minolfa C. TITLE=Low Nitric Oxide Bioavailability Increases Renin Production in the Collecting Duct JOURNAL=Frontiers in Physiology VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.559341 DOI=10.3389/fphys.2020.559341 ISSN=1664-042X ABSTRACT=
In the kidney, the stimulation of renin production by the collecting duct (CD-renin) contributes to the development of hypertension. The CD is a major nephron segment for the synthesis of nitric oxide (NO), and low NO bioavailability in the renal medulla is associated with hypertension. However, it is unknown whether NO regulates renin production in the CD. To test the hypothesis that low intrarenal NO levels stimulate the production of CD-renin, we first examined renin expression in the distal nephron segments of CD-eNOS deficient mice. In these mice, specific CD-renin immunoreactivity was increased compared to wild-type littermates; however, juxtaglomerular (JG) renin was not altered. To further assess the intracellular mechanisms involved, we then treated M-1 cells with either 1 mM L-NAME (L-arginine analog), an inhibitor of NO synthase activity, or 1 mM NONOate, a NO donor. Both treatments increased intracellular renin protein levels in M-1 cells. However, only the inhibition of NOS with L-NAME stimulated renin synthesis and secretion as reflected by the increase in