AUTHOR=Nie Zhaojun , Yeh Shu-Chi Allison , LePalud Michelle , Badr Fares , Tse Frances , Armstrong David , Liu Louis W. C. , Deen M. Jamal , Fang Qiyin 

TITLE=Optical Biopsy of the Upper GI Tract Using Fluorescence Lifetime and Spectra

JOURNAL=Frontiers in Physiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/physiology/articles/10.3389/fphys.2020.00339

DOI=10.3389/fphys.2020.00339

ISSN=1664-042X

ABSTRACT=<p>Screening and surveillance for gastrointestinal (GI) cancers by endoscope guided biopsy is invasive, time consuming, and has the potential for sampling error. Tissue endogenous fluorescence spectra contain biochemical and physiological information, which may enable real-time, objective diagnosis. We first briefly reviewed optical biopsy modalities for GI cancer diagnosis with a focus on fluorescence-based techniques. In an <italic>ex vivo</italic> pilot clinical study, we measured fluorescence spectra and lifetime on fresh biopsy specimens obtained during routine upper GI screening procedures. Our results demonstrated the feasibility of rapid acquisition of time-resolved fluorescence (TRF) spectra from fresh GI mucosal specimens. We also identified spectroscopic signatures that can differentiate between normal mucosal samples obtained from the esophagus, stomach, and duodenum.</p>